Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,033,499	A→G	V82V (GTA→GTG)	hyaB →	hydrogenase 1 large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,033,499	0	A	G	100.0%	286.5 / NA	79	V82V (GTA→GTG)	hyaB	hydrogenase 1 large subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/39);  total (40/39)

CGATCAGAATGTGATCACCAATGCCGTCTCCTGCGGCACCATGTTTCGCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTACACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTATGCTGGCAACGCTCTGGTGCCACGATCATCTGGTGCACTTCTATCAGCTTGC  >  NC_000913/1033367‑1033633                                                                                                                                     |                                                                                                                                       cGATCAGAATGTGATCACCAATGCCGTCTCCTGCGGCACCATGTTTCGCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGccc                                                                                                                                 <  2:866682/140‑1 (MQ=255)   aTCAGAATGTGATCACCAATGCCGTCTCCTGCGGCACCATGTTTCGCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTg                                                                                                                               >  1:170815/1‑140 (MQ=255)   aTCAGAATGTGATCACCAATGCCGTCTCCTGCGGCACCATGTTTCGCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTg                                                                                                                               >  1:201268/1‑140 (MQ=255)       gAATGTGATCACCAATGCCGTCTCCTGCGGCACCATGTTTCGCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCtt                                                                                                                           >  1:165684/1‑140 (MQ=255)        aaTGTGATCACCAATGCCGTCTCCTGCGGCACCATGTTTCGCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTc                                                                                                                          >  2:456557/1‑140 (MQ=255)          tgtgATCACCAATGCCGTCTCCTGCGGCACCATGTTTCGCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCgg                                                                                                                        >  1:827247/1‑140 (MQ=255)              aTCACCAATGCCGTCTCCTGCGGCACCATGTTTCGCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTa                                                                                                                    >  2:162155/1‑140 (MQ=255)                  ccAATGCCGTCTCCTGCGGCACCATGTTTCGCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGcc                                                                                                                >  2:865467/1‑140 (MQ=255)                  ccAATGCCGTCTCCTGCGGCACCATGTTTCGCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGcc                                                                                                                >  1:872308/1‑140 (MQ=255)                  ccAATGCCGTCTCCTGCGGCACCATGTTTCGCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGcc                                                                                                                >  2:677665/1‑140 (MQ=255)                  ccAATGCCGTCTCCTGCGGCACCATGTTTCGCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGcc                                                                                                                >  1:334549/1‑140 (MQ=255)                  ccAATGCCGTCTCCTGCGGCACCATGTTTCGCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGcc                                                                                                                >  2:248301/1‑140 (MQ=255)                      tGCCGTCTCCTGCGGCACCATGTTTCGCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACACCATCg                                                                                                            >  2:146713/1‑140 (MQ=255)                      tGCCGGCTCCTGCGGCACCATGTTTCGCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCg                                                                                                            >  2:401668/1‑140 (MQ=255)                       gCCGTCTCCTGCGGCACCATGTTTCGCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGa                                                                                                           <  2:170815/140‑1 (MQ=255)                             tcCTGCGGCACCATGTTTCGCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGc                                                                                                     >  2:196466/1‑140 (MQ=255)                             tcCTGCGGCACCATGTTTCGCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGc                                                                                                     >  1:696474/1‑140 (MQ=255)                              ccTGCGGCACCATGTTTCGCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCt                                                                                                    <  1:575546/140‑1 (MQ=255)                                tGCGGCACCATGTTTCGCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTAt                                                                                                  <  1:394243/140‑1 (MQ=255)                                tGCGGCACCATGTTTCGCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTAt                                                                                                  <  1:456557/140‑1 (MQ=255)                                   ggCACCATGTTTCGCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCgg                                                                                               <  2:696474/140‑1 (MQ=255)                                     cACCATGTTTCGCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTa                                                                                             >  2:314422/1‑140 (MQ=255)                                       ccATGTTTCGCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTAt                                                                                                  <  1:436350/133‑1 (MQ=255)                                       ccATGTTTCGCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTAt                                                                                                  >  2:436350/1‑133 (MQ=255)                                       ccATGTTTCGCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTAtt                                                                                           >  1:455214/1‑140 (MQ=255)                                         aTGTTTCGCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTaa                                                                                         >  2:43948/1‑140 (MQ=255)                                         aTGTTTCGCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTaa                                                                                         >  2:921502/1‑140 (MQ=255)                                                 cgGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCgg                                                                                 >  1:834227/1‑140 (MQ=255)                                                   ggCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGAc                                                                               <  1:921502/140‑1 (MQ=255)                                                   ggCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGAc                                                                               <  1:43948/140‑1 (MQ=255)                                                   ggCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACACCATCGAAGATGCTATCGGTATTAAAGTGCCGGAc                                                                               <  1:146713/140‑1 (MQ=255)                                                    gCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACa                                                                              >  2:136647/1‑140 (MQ=255)                                                              atcCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGAc                                                                               >  1:88002/1‑129 (MQ=255)                                                              atcCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGAc                                                                               <  2:88002/129‑1 (MQ=255)                                                              atcCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAatat                                                                    >  1:430131/1‑140 (MQ=255)                                                                    cAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCg                                                              <  2:165684/140‑1 (MQ=255)                                                                      aGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCa                                                            <  2:834227/140‑1 (MQ=255)                                                                        ggCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAAc                                                          >  2:271860/1‑140 (MQ=255)                                                                        ggCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAAc                                                          <  1:248301/140‑1 (MQ=255)                                                                         gcgcGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACa                                                         <  2:430131/140‑1 (MQ=255)                                                                          cgcgACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACa                                                         <  1:688583/139‑1 (MQ=255)                                                                          cgcgACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACa                                                         >  1:599885/1‑139 (MQ=255)                                                                          cgcgACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACa                                                         >  2:688583/1‑139 (MQ=255)                                                                          cgcgACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACa                                                         <  2:599885/139‑1 (MQ=255)                                                                          cgcgACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACAt                                                        >  1:790804/1‑140 (MQ=255)                                                                          cgcgACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACAt                                                        >  2:917237/1‑140 (MQ=255)                                                                          cgcgACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACAt                                                        >  1:76986/1‑140 (MQ=255)                                                                          cgcgACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACAt                                                        >  2:184700/1‑140 (MQ=255)                                                                          cgcgACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACAt                                                        >  1:328242/1‑140 (MQ=255)                                                                           gcgACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACAtt                                                       <  1:184700/140‑1 (MQ=255)                                                                           gcgACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACAtt                                                       >  2:535109/1‑140 (MQ=255)                                                                            cgACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTa                                                      >  2:851746/1‑140 (MQ=255)                                                                              aCCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTATg                                                    <  1:851746/140‑1 (MQ=255)                                                                               cccGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTATGc                                                   <  2:246503/140‑1 (MQ=255)                                                                               cccGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTATGc                                                   <  2:827247/140‑1 (MQ=255)                                                                               cccGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTATGc                                                   <  1:136647/140‑1 (MQ=255)                                                                                       aTGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCTAATATCATCCGCAACATTATGCTGGCAACg                                           <  1:863665/140‑1 (MQ=255)                                                                                       aTGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTATGCTGGCAACg                                           <  2:872308/140‑1 (MQ=255)                                                                                               gCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTATGCTGGCAACGCTCTGGTg                                   <  1:401668/140‑1 (MQ=255)                                                                                               gCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTATGCTGGCAACGCTCTGGTg                                   <  2:201268/140‑1 (MQ=255)                                                                                                cgttcgttGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTATGCTGGCAACGCTCTGGTGc                                  <  1:271860/140‑1 (MQ=255)                                                                                                cgttcgttGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTATGCTGGCAACGCTCTGGTGc                                  <  1:386907/140‑1 (MQ=255)                                                                                                  ttcgttGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTATGCTGGCAACGCTCTGGTGCCa                                >  1:794288/1‑140 (MQ=255)                                                                                                  ttcgttGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTATGCTGGCAACGCTCTGGTGCCa                                >  1:888453/1‑140 (MQ=255)                                                                                                  ttcgttGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTATGCTGGCAACGCTCTGGTGCCa                                <  1:162155/140‑1 (MQ=255)                                                                                                  ttcgttGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTATGCTGGCAACGCTCTGGTGCCa                                <  1:917237/140‑1 (MQ=255)                                                                                                  ttcgttGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTATGCTGGCAACGCTCTGGTGCCa                                <  2:334549/140‑1 (MQ=255)                                                                                                  ttcgttGAACGTATCTGCGGCGTCTGTACTGGCGTGCACGCCATGGCTTCGGTTTACGACATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTATGCTGGCAACGCTCTGGTGCCa                                <  2:888453/140‑1 (MQ=255)                                                                                                              aTCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTATGCTGGCAACGCTCTGGTGCCACGATCATCTGGt                    >  2:686684/1‑140 (MQ=255)                                                                                                              aTCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTATGCTGGCAACGCTCTGGTGCCACGATCATCTGGt                    <  1:686684/140‑1 (MQ=255)                                                                                                              aTCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTATGCTGGCAACGCTCTGGTGCCACGATCATCTGGt                    <  2:455214/140‑1 (MQ=255)                                                                                                              aTCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCAAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTATGCTGGCAACGCTCTGGTGCCACGATCATCTGGt                    <  1:661762/140‑1 (MQ=255)                                                                                                               tCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTATGCTGGCAACGCTCTGGTGCCACGATCATCTGGTg                   <  2:790804/140‑1 (MQ=255)                                                                                                               tCTGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTATGCTGGCAACGCTCTGGTGCCACGATCATCTGGTg                   >  1:508591/1‑140 (MQ=255)                                                                                                                 tGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTATGCTGGCAACGCTCTGGTGCCACGATCATCTGGTGCa                 <  1:196466/140‑1 (MQ=255)                                                                                                                 tGCGGCGTCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTATGCTGGCAACGCTCTGGTGCCACGATCATCTGGTGCa                 <  2:76986/140‑1 (MQ=255)                                                                                                                        tCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTATGCTGGCAACGCTCTGGTGCCACGATCATCTGGTGc                  <  1:546749/132‑1 (MQ=255)                                                                                                                        tCTGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTATGCTGGCAACGCTCTGGTGCCACGATCATCTGGTGc                  >  2:546749/1‑132 (MQ=255)                                                                                                                          tGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGTCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTATGCTGGCAACGCTCTGGTGCCACGATCATCTGGTGCACTTCTa           >  2:831577/1‑137 (MQ=255)                                                                                                                          tGTACTGGCGTGCACGCCCTGGCTTCGGTTTACGTCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTATGCTGGCAACGCTCTGGTGCCACGATCATCTGGTGCACTTCTa           <  1:831577/137‑1 (MQ=255)                                                                                                                                ggCGTGCACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTATGCTGGCAACGCTCTGGTGCCACGATCATCTGGTGCACTTCTATCAGCTTGc  >  2:246458/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                       CGATCAGAATGTGATCACCAATGCCGTCTCCTGCGGCACCATGTTTCGCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACTGGCGTACACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCGGACAACGCCAATATCATCCGCAACATTATGCTGGCAACGCTCTGGTGCCACGATCATCTGGTGCACTTCTATCAGCTTGC  >  NC_000913/1033367‑1033633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑