Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	124,133	G→A	A373T (GCA→ACA)	aceE →	pyruvate dehydrogenase E1 component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	124,133	0	G	A	100.0%	154.4 / NA	44	A373T (GCA→ACA)	aceE	pyruvate dehydrogenase E1 component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/20);  total (24/20)

GGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGA  >  NC_000913/124007‑124263                                                                                                                               |                                                                                                                                   ggTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAgc                                                                                                                       >  2:181419/1‑140 (MQ=255)   tGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAgcgc                                                                                                                     >  1:104368/1‑140 (MQ=255)           ttCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAAcc                                                                                                             <  1:601800/140‑1 (MQ=255)             cGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCaa                                                                                                           >  2:258915/1‑140 (MQ=255)                      ttcttcGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGc                                                                                                  <  1:574076/140‑1 (MQ=255)                      ttcttcGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGc                                                                                                  <  1:783679/140‑1 (MQ=255)                      ttcttcGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGc                                                                                                  >  1:723900/1‑140 (MQ=255)                      ttcttcGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGc                                                                                                  <  2:723900/140‑1 (MQ=255)                      ttcttcGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGc                                                                                                  >  2:574076/1‑140 (MQ=255)                      ttcttcGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGc                                                                                                  >  2:787973/1‑140 (MQ=255)                      ttcttcGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGc                                                                                                  >  2:882740/1‑140 (MQ=255)                       tcttcGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCg                                                                                                 <  1:548221/140‑1 (MQ=255)                        cttcGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGa                                                                                                >  1:130210/1‑140 (MQ=255)                            ggTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGt                                                                                            <  1:837222/140‑1 (MQ=255)                                aaTATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGCCAGTAATc                                                                                        >  1:801664/1‑140 (MQ=255)                                aaTATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATc                                                                                        <  1:808158/140‑1 (MQ=255)                                 atatCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATcc                                                                                       <  1:258915/140‑1 (MQ=255)                                        gAAACCGCAGCACTGGTTGCAGACTGTACTGACGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCa                                                                                >  2:42946/1‑140 (MQ=255)                                           aCCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAGAGGCAAAGCGACAGTCATCCTTGCTCAGAc                                                                             >  2:590341/1‑140 (MQ=255)                                              gcagcaCTGGTTGCAGACTGGACTGACGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTg                                                                                    <  1:598567/130‑1 (MQ=255)                                              gcagcaCTGGTTGCAGACTGGACTGACGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTg                                                                                    >  2:598567/1‑130 (MQ=255)                                                  caCTGGTTGCAGACTGGACTGACGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTaaa                                                                      >  1:636840/1‑140 (MQ=255)                                                      ggTTGCAGACTGGACTGACGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGtt                                                                  <  1:732740/140‑1 (MQ=255)                                                      ggTTGCAGACTGGACTGACGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGtt                                                                  >  1:290626/1‑140 (MQ=255)                                                       gTTGCAGACTGGACTGACGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTa                                                                 >  2:346246/1‑140 (MQ=255)                                                                tgtactgACGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATggg                                                        <  1:42946/137‑1 (MQ=255)                                                                                         tGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGaa                                           >  1:359585/1‑128 (MQ=255)                                                                                         tGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGaa                                           <  2:359585/128‑1 (MQ=255)                                                                                         tGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATc                               >  2:7098/1‑140 (MQ=255)                                                                                          gAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATcg                              <  2:636840/140‑1 (MQ=255)                                                                                          gAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATcg                              <  2:937310/140‑1 (MQ=255)                                                                                           aaCCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATcgc                             >  1:668622/1‑140 (MQ=255)                                                                                           aaCCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATcgc                             >  1:462140/1‑140 (MQ=255)                                                                                           aaCCGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATcgc                             >  1:109780/1‑140 (MQ=255)                                                                                             ccGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCgcgc                           <  1:590341/140‑1 (MQ=255)                                                                                              cGTGGTGGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCa                          <  2:130210/140‑1 (MQ=255)                                                                                               gtggtgGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGCCGCGGCTGAAGGTAAAACCATCGCGCAc                         <  2:246215/140‑1 (MQ=255)                                                                                               gtggtgGTCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCAc                         <  1:181419/140‑1 (MQ=255)                                                                                                      tCACGATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTa                  <  2:104368/140‑1 (MQ=255)                                                                                                          gATCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGaa              >  2:537236/1‑140 (MQ=255)                                                                                                            tCCGAAGAAAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGaaaa            >  2:508328/1‑140 (MQ=255)                                                                                                               gaagaaAATCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGa         >  2:740314/1‑140 (MQ=255)                                                                                                                      aTCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGCCAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGATAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGa  <  2:801664/140‑1 (MQ=255)                                                                                                                      aTCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAg                >  2:193496/1‑126 (MQ=255)                                                                                                                      aTCTACGCTACATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAg                <  1:193496/126‑1 (MQ=255)                                                                                                                               |                                                                                                                                   GGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGA  >  NC_000913/124007‑124263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑