Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,157,016	A→G	L80L (TTA→TTG)	ycfH →	putative metal‑dependent hydrolase YcfH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,157,016	0	A	G	100.0%	190.1 / NA	54	L80L (TTA→TTG)	ycfH	putative metal‑dependent hydrolase YcfH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/26);  total (28/26)

TCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAAC  >  NC_000913/1156895‑1157148                                                                                                                          |                                                                                                                                     tCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCagaa                                                                                                                    <  1:137916/140‑1 (MQ=255) tCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCagaa                                                                                                                    <  2:143915/140‑1 (MQ=255)       caacaTTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTggcgg                                                                                                                         <  2:873126/129‑1 (MQ=255)       caacaTTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTggcgg                                                                                                                         >  1:873126/1‑129 (MQ=255)       caacaTTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGgt                                                                                                              >  1:131925/1‑140 (MQ=255)               ccGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAgc                                                                                                      >  1:665682/1‑140 (MQ=255)               ccGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAgc                                                                                                      >  2:459593/1‑140 (MQ=255)                  ggTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCt                                                                                                   >  2:692670/1‑140 (MQ=255)                    ttATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGGGTTGTAGCGCTgg                                                                                                 >  1:789368/1‑140 (MQ=255)                      aTTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGt                                                                                               >  2:673998/1‑140 (MQ=255)                      aTTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGt                                                                                               >  1:926620/1‑140 (MQ=255)                      aTTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGt                                                                                               >  1:108078/1‑140 (MQ=255)                       tttACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTg                                                                                              >  2:803908/1‑140 (MQ=255)                       tttACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTg                                                                                              >  2:921998/1‑140 (MQ=255)                                gCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGc                                                                                     <  1:635476/140‑1 (MQ=255)                                 cGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAgcg                                                                                                     <  1:944920/123‑1 (MQ=255)                                 cGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAgcg                                                                                                     >  2:944920/1‑123 (MQ=255)                                 cGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCggg                                                                                      <  2:406215/138‑1 (MQ=255)                                 cGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCggg                                                                                      >  1:406215/1‑138 (MQ=255)                                   ggATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTgg                                                                                  <  2:789368/140‑1 (MQ=255)                                   ggATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTgg                                                                                  >  2:781027/1‑140 (MQ=255)                                   ggATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTgg                                                                                  <  2:131925/140‑1 (MQ=255)                                         ggTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGattatt                                                                            >  2:316975/1‑140 (MQ=255)                                             ggCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGAttattatta                                                                        <  2:665682/140‑1 (MQ=255)                                              gCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTgg                                                                                  >  1:261231/1‑129 (MQ=255)                                              gCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTgg                                                                                  <  2:261231/129‑1 (MQ=255)                                               cGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTaca                                                                      <  2:154876/140‑1 (MQ=255)                                               cGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTaca                                                                      >  1:782675/1‑140 (MQ=255)                                                   cGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGcc                                                                  <  2:782675/140‑1 (MQ=255)                                                    gTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCg                                                                 >  2:497583/1‑140 (MQ=255)                                                             tcgtATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTaaa                                                        <  1:921998/140‑1 (MQ=255)                                                              cgtATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAg                                                       <  1:316975/140‑1 (MQ=255)                                                              cgtATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAg                                                       <  2:535760/140‑1 (MQ=255)                                                               gtATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGt                                                      <  1:781027/140‑1 (MQ=255)                                                                tATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTa                                                     >  1:552914/1‑140 (MQ=255)                                                                  ttttCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACg                                                   <  2:108078/140‑1 (MQ=255)                                                                       ttGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGc                                              >  2:340308/1‑140 (MQ=255)                                                                       ttGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGc                                              <  1:497583/140‑1 (MQ=255)                                                                        tgtgGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCa                                             >  1:818954/1‑140 (MQ=255)                                                                          tgGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAag                                           >  1:301201/1‑140 (MQ=255)                                                                                gCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCt                                     <  1:692670/140‑1 (MQ=255)                                                                                    ccACTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTcat                                 <  1:340308/140‑1 (MQ=255)                                                                                    ccACTCAACCAGAATGATCCCTACGACGTAGAAGATTTGCGCCGTCCGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTcat                                 <  1:673998/140‑1 (MQ=255)                                                                                      aCTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTcc                                      >  2:125925/1‑133 (MQ=255)                                                                                      aCTCAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTcc                                      <  1:125925/133‑1 (MQ=255)                                                                                         cAACCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTcatccatc                            <  1:459593/140‑1 (MQ=255)                                                                                          aaCCAGAATGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCcatca                           >  1:361817/1‑140 (MQ=255)                                                                                                aaTGATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCa                     >  1:450699/1‑140 (MQ=255)                                                                                                   gATCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGAt                  <  2:301201/140‑1 (MQ=255)                                                                                                    aTCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAATTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGAtt                 >  1:118987/1‑140 (MQ=255)                                                                                                    aTCCCTACGATGTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGAtt                 >  1:859017/1‑140 (MQ=255)                                                                                                               gTAGAAGATTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGtgaact      <  2:267971/140‑1 (MQ=255)                                                                                                                  gaagaTTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAATTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGtgaactgaa   <  2:118987/140‑1 (MQ=255)                                                                                                                   aagaTTTGCGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGtgaactgaac  >  2:500782/1‑140 (MQ=255)                                                                                                                          |                                                                                                                                     TCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAAC  >  NC_000913/1156895‑1157148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑