Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,162,230	C→T	H116Y (CAT→TAT)	hinT →	purine nucleoside phosphoramidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,162,230	0	C	T	100.0%	131.2 / NA	38	H116Y (CAT→TAT)	hinT	purine nucleoside phosphoramidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/18);  total (20/18)

CAAAAATTGCTGAGCAAGAAGGTATTGCCGAAGATGGCTATCGTCTGATCATGAATACCAACCGCCATGGCGGACAAGAGGTTTACCACATCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGCATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTGGTGTCTCTGGTGTTGTTACTGCTGGTGGGCTGTCGTTCACATCCGGAAATTCCGGTGAATGATGAGCAATCGCTGGTGATGGAGT  >  NC_000913/1162093‑1162355                                                                                                                                          |                                                                                                                              cAAAAATTGCTGAGCAAGAAGGTATTGCCGAAGATGGCTATCGTCTGATCATGAATACCAACCGCCATGGCGGACAAGAGGTTTACCACATCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTAt                                                                                                                             >  2:86920/1‑140 (MQ=255)  aaaaaTTGCTGAGCAAGAAGGTATTGCCGAAGATGGCTATCGTCTGATCATGAATACCAACCGCCATGGCGGACAAGAGGTTTACCACATCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGtata                                                                                                                            <  2:115290/140‑1 (MQ=255)  aaaaaTTGCTGAGCAAGAAGGTATTGCCGAAGATGGCTATCGTCTGATCATGAATACCAACCGCCATGGCGGACAAGAGGTTTACCACATCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGtata                                                                                                                            <  1:86920/140‑1 (MQ=255)   aaaaTTGCTGAGAAAGAAGGTATTGCCGAAGATGGCTATCGTCTGATCATGAATCCCAACCGCCATGGCGGACAAGAGGTTTACCACATCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATaa                                                                                                                           <  2:382710/140‑1 (MQ=255)     aaTTGCTGAGCAAGAAGGATTTGCCGAAGATGGCTATCGTCTGATAATGAATCCCAACCGCCATGGCGGACAAGAGGTTTACCACATCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAg                                                                                                                         <  2:100033/140‑1 (MQ=255)              gCAAGAAGGTATTGCCGAAGATGGCTATCGTCTGATCATGAATACCAACCGCCATGGCGGACAAGAGGTTTACCACATCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAAc                                                                                                                <  2:51851/140‑1 (MQ=255)                          tGCCGAAGATGGCTATCGTCTGATCATGAATACCAACCGCCATGGCGGACAAGAGGTTTACCACATCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAgg                                                                                                    <  2:852559/140‑1 (MQ=255)                                 gATGGCTATCGTCTGATCATGAATACCAACCGCCATGGCGGACAAGAGGTTTACCACATCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCttt                                                                                             >  2:307085/1‑140 (MQ=255)                                    ggCTATCGTCTGATCATGAATACCAACCGCCATGGCGGACAAGAGGTTTACCACATCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTggg                                                                                          >  2:350085/1‑140 (MQ=255)                                      cTATCGTCTGATCATGAATACCAACCGCCATGGCGGACAAGAGGTTTACCACATCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCt                                                                                        >  2:676638/1‑140 (MQ=255)                                        aTCGTCTGATCATGAATACCAACCGCCATGGCGGACAAGAGGTTTACCACATCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTgg                                                                                      <  2:758182/140‑1 (MQ=255)                                         tCGTCTGATCATGAATACCAACCGCCATGGCGGACAAGAGGTTTACCACATCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTGgt                                                                                     <  2:188598/140‑1 (MQ=255)                                         tCGTCTGATCATGAATACCAACCGCCATGGCGGACAAGAGGTTTACCACATCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTGgt                                                                                     <  1:612678/140‑1 (MQ=255)                                          cGTCTGATCATGAATACCAACCGCCATGGCGGACAAGAGGTTTACCACATCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTGgtg                                                                                    >  1:389676/1‑140 (MQ=255)                                           gTCTGATCATGAATACCAACCGCCATGGCGGACAAGAGGTTTACCACATCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTGgtgt                                                                                   <  2:615956/140‑1 (MQ=255)                                            tCTGATCATGAATACCAACCGCCATGGCGGACAAGAGGTTTACCACATCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTGGTGtc                                                                                  >  2:636843/1‑140 (MQ=255)                                               gATCATGAATACCAACCGCCATGGCGGACAAGAGGTTTACCACATCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTGGTGTctct                                                                               <  1:180643/140‑1 (MQ=255)                                                aTCATGAATACCAACCGCCATGGCGGACAAGAGGTTTACCACATCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTGGTGTCTCTg                                                                              >  1:925668/1‑140 (MQ=255)                                                    tGAATACCAACCGCCATGGCGGACAAGAGGTTTACCACATCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTGGTGTCTCTgg                                                                             >  1:308377/1‑137 (MQ=255)                                                    tGAATACCAACCGCCATGGCGGACAAGAGGTTTACCACATCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTGGTGTCTCTgg                                                                             <  2:308377/137‑1 (MQ=255)                                                     gAATACCAACCGCCATGGCGGACAAGAGGTTTACCACATCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTGGTGTCTCTGGtgtt                                                                         <  2:92585/140‑1 (MQ=255)                                                      aaTACCAACCGCCATGGCGGACAAGAGGTTTACCACATCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTGGTGTCTCTGGtgttg                                                                        >  2:388271/1‑140 (MQ=255)                                                      aaTACCAACCGCCATGGCGGACAAGAGGTTTACCACATCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTGGTGTCTCTGGtgttg                                                                        >  2:934723/1‑140 (MQ=255)                                                          ccAACCGCCATGGCGGACAAGAGGTTTACCACATCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTGGTGTCTCTGGTGTTGTTAc                                                                    >  2:390789/1‑140 (MQ=255)                                                          ccAACCGCCATGGCGGACAAGAGGTTTACCACATCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTGGTGTCTCTGGTGTTGTTAc                                                                    >  1:462274/1‑140 (MQ=255)                                                          ccAACCGCCATGGCGGACAAGAGGTTTACCACATCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTGGTGTCTCTGGTGTTGTTAc                                                                    <  1:350085/140‑1 (MQ=255)                                                             aCCGCCATGGCGGACAAGAGGTTTACCACATCCATATGCACTTGTTTGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTGGTGTCTCTGGTGTTGTTActgc                                                                 >  1:657135/1‑140 (MQ=255)                                                                  cATGGCGGACAAGAGGTTTACCACATCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTGGTGTCTCTGGTGTTGTTACTGCtggtg                                                            <  1:390789/140‑1 (MQ=255)                                                                            aaGAGGTTTACCACATCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTGGTGTCTCTGGTGTTGTTACTGCTGGTGGGCTGTCGtt                                                  <  2:363192/140‑1 (MQ=255)                                                                                           tCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTGGTGTCTCTGGTGTTGTTACTGCTGGTGGGCTGTCGTTCACATCCgg                                         <  1:792854/134‑1 (MQ=255)                                                                                           tCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTGGTGTCTCTGGTGTTGTTACTGCTGGTGGGCTGTCGTTCACATCCgg                                         >  2:792854/1‑134 (MQ=255)                                                                                                 tGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTGGTGTCTCTGGTGTTGTTACTGCTGGTGGGCTGTCGTTCACATCCGGAAATTCCGGTGa                             >  2:286505/1‑140 (MQ=255)                                                                                                 tGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGCGCTGGTGTCTCTGGTGTTGTTACTGCTGGTGGGCTGTCGTTCACATCCGGAAATTCCGGTGa                             >  1:322963/1‑140 (MQ=255)                                                                                                    aCTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTGGTG‑‑TCTGGTGTTGTTACTGCTGGTGGGCTGTCGTTCACATCCGGAAATTCCGGTGAatgat                        >  1:350548/1‑140 (MQ=255)                                                                                                      ttgttgGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTGGTGTCTCTGGTGTTGTTACTGCTGGTGGGCTGTCGTTCACATCCGGAAATTCCGGTGAatgat                        >  2:534437/1‑140 (MQ=255)                                                                                                        gttgGGTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTGGTGTCTCTGGTGTTGTTACTGCTGGTGGGCTGTCGTTCACATCCGGAAATTCCGGTGAAtgatga                      >  1:902291/1‑140 (MQ=255)                                                                                                            ggTGGCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTGGTGTCTCTGGTGTTGTTACTGCTGGTGGGCTGTCGTTCACATCCGGAAATTCCGGTGAATGATGAGCaa                  <  1:676638/140‑1 (MQ=255)                                                                                                                gCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTGGTGTCTCTGGTGTTGTTACTGCTGGTGGGCTGTCGTTCACATCCGGAAATTCCGGTGAATGATGAGCAATCGc              <  1:934723/140‑1 (MQ=255)                                                                                                                gCCGTCCGCTGGGACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTGGTGTCTCTGGTGTTGTTACTGCTGGTGGGCTGTCGTTCACATCCGGAAATTCCGGTGAATGATGAGCAATCGc              >  1:342048/1‑140 (MQ=255)                                                                                                                            gACCAATGCTGGCGTATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTGGTGTCTCTGGTGTTGTTACTGCTGGTGGGCTGTCGTTCACATCCGGAAATTCCGGTGAATGATGAGCAATCGCTGGTGATGGAGt  <  1:307085/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                              CAAAAATTGCTGAGCAAGAAGGTATTGCCGAAGATGGCTATCGTCTGATCATGAATACCAACCGCCATGGCGGACAAGAGGTTTACCACATCCATATGCACTTGTTGGGTGGCCGTCCGCTGGGACCAATGCTGGCGCATAAAGGTCTGTAACGATGAGAAAAGGATGCTTTGGGCTGGTGTCTCTGGTGTTGTTACTGCTGGTGGGCTGTCGTTCACATCCGGAAATTCCGGTGAATGATGAGCAATCGCTGGTGATGGAGT  >  NC_000913/1162093‑1162355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑