Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,220,971	G→A	pseudogene (1371/2648 nt)	ycgH →	putative transporter component, N‑terminal fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,220,971	0	G	A	100.0%	180.8 / NA	51	pseudogene (1371/2648 nt)	ycgH	putative transporter component, N‑terminal fragment
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/26);  total (25/26)

AGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCGGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAATCTC  >  NC_000913/1220839‑1221101                                                                                                                                     |                                                                                                                                   aGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACgg                                                                                                                             <  2:255643/140‑1 (MQ=255)  gACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGa                                                                                                                            <  2:142330/140‑1 (MQ=255)   aCAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGAc                                                                                                                           >  2:388686/1‑140 (MQ=255)   aCAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGAc                                                                                                                           >  1:550198/1‑140 (MQ=255)   aCAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGAc                                                                                                                           <  1:919239/140‑1 (MQ=255)     aGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAg                                                                                                                         <  2:863779/140‑1 (MQ=255)     aGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAg                                                                                                                         <  2:45548/140‑1 (MQ=255)             cAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTAttg                                                                                                                 >  2:723348/1‑140 (MQ=255)               aTCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTAttgtt                                                                                                               <  2:462611/140‑1 (MQ=255)               aTCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTAttgtt                                                                                                               <  1:388686/140‑1 (MQ=255)               aTCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTAttgtt                                                                                                               <  1:451825/140‑1 (MQ=255)               aTCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTAttgtt                                                                                                               <  2:285014/140‑1 (MQ=255)               aTCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTAttgtt                                                                                                               >  1:922236/1‑140 (MQ=255)                tCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTAttgttg                                                                                                              <  1:339347/140‑1 (MQ=255)                 cTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGa                                                                                                             <  2:922236/140‑1 (MQ=255)                 cTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGa                                                                                                             >  1:84145/1‑140 (MQ=255)                 cTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGa                                                                                                             >  2:148553/1‑140 (MQ=255)                                ggTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCggg                                                                                              <  1:698286/140‑1 (MQ=255)                                  tCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGtt                                                                                            <  2:22583/140‑1 (MQ=255)                                  tCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGtt                                                                                            <  2:215191/140‑1 (MQ=255)                                     aaCATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCaa                                                                                         >  2:527671/1‑140 (MQ=255)                                           tACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCg                                                                                   >  1:524547/1‑140 (MQ=255)                                            aCCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGt                                                                                  <  1:723348/140‑1 (MQ=255)                                                       aaCATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGaaaa                                                                       <  2:569808/140‑1 (MQ=255)                                                            tAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGaa                                                                         >  1:636243/1‑133 (MQ=255)                                                            tAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGaa                                                                         <  2:636243/133‑1 (MQ=255)                                                                    aaaGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGTTGGAGGAAg                                                          <  1:527671/140‑1 (MQ=255)                                                                             tATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAgcggcgcggc                                                 <  1:540300/140‑1 (MQ=255)                                                                             tATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAgcggcgcggc                                                 <  2:831063/140‑1 (MQ=255)                                                                              aTGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGcc                                                <  2:84145/140‑1 (MQ=255)                                                                                 tCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACg                                             >  1:583200/1‑140 (MQ=255)                                                                                   ggTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCt                                           >  2:394184/1‑140 (MQ=255)                                                                                             gTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATc                                 <  2:583200/140‑1 (MQ=255)                                                                                             gTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATc                                 <  2:524547/140‑1 (MQ=255)                                                                                             gTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATc                                 >  2:576377/1‑140 (MQ=255)                                                                                             gTCAGCTTAATATCCGCACCGTATTAGGTGATGACAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATc                                 <  1:362202/140‑1 (MQ=255)                                                                                             gTCAGCTTAATATCCGCACCGTATTAGGAGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATc                                 >  2:513582/1‑140 (MQ=255)                                                                                              tCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCg                                >  1:850231/1‑140 (MQ=255)                                                                                                aGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGaa                              >  1:366813/1‑140 (MQ=255)                                                                                                  cTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACt                            >  2:100793/1‑140 (MQ=255)                                                                                                  cTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACt                            >  1:628602/1‑140 (MQ=255)                                                                                                  cTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACt                            <  1:394184/140‑1 (MQ=255)                                                                                                            gCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACtgtg                  >  1:642017/1‑140 (MQ=255)                                                                                                                     tAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGAt         <  2:547793/140‑1 (MQ=255)                                                                                                                          gatgatAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAAtc    >  1:91597/1‑140 (MQ=255)                                                                                                                          gatgatAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAAtc    >  2:380853/1‑140 (MQ=255)                                                                                                                          gatgatAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAAtc    >  1:602702/1‑140 (MQ=255)                                                                                                                          gatgatAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAAtc    >  2:926725/1‑140 (MQ=255)                                                                                                                            tgatAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAAtctc  >  2:401595/1‑140 (MQ=255)                                                                                                                            tgatAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAAtctc  >  1:617426/1‑140 (MQ=255)                                                                                                                            tgatAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAAtctc  >  1:263600/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                   AGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCGGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAATCTC  >  NC_000913/1220839‑1221101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑