Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,245,222	T→C	S21S (AGT→AGC)	ycgY →	uncharacterized protein YcgY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,245,222	0	T	C	100.0%	158.3 / NA	44	S21S (AGT→AGC)	ycgY	uncharacterized protein YcgY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/21);  total (23/21)

TTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAA  >  NC_000913/1245085‑1245355                                                                                                                                          |                                                                                                                                      ttCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGa                                                                                                                                     >  1:924531/1‑140 (MQ=255)   cGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAgg                                                                                                                                   >  2:238480/1‑140 (MQ=255)   cGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAgg                                                                                                                                   >  2:108324/1‑140 (MQ=255)           gATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTg                                                                                                                           >  1:491222/1‑140 (MQ=255)             tAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGtttttgg                                                                                                                         >  2:857500/1‑137 (MQ=255)             tAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTGGc                                                                                                                         <  2:396477/140‑1 (MQ=255)             tAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTGGc                                                                                                                         <  1:304707/140‑1 (MQ=255)             tAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTGGc                                                                                                                         >  1:396477/1‑140 (MQ=255)             tAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGGTTTggg                                                                                                                         >  1:941519/1‑139 (MQ=255)                    ggTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTGGCTGCGCtt                                                                                                                  >  2:927401/1‑140 (MQ=255)                      ttCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTGGCTGCGCTTAt                                                                                                                <  1:303306/140‑1 (MQ=255)                         ccGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTGGCTGCGCTTATTGa                                                                                                             <  1:553539/140‑1 (MQ=255)                         ccGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTGGCTGCGCTTATTGa                                                                                                             <  2:924531/140‑1 (MQ=255)                           ggTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTGGCTGCGCTTATTGAAc                                                                                                           <  1:530991/140‑1 (MQ=255)                                caacaacaGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTGGTTGGGCTTTTTTAAAATTTa                                                                                                      >  2:553539/1‑140 (MQ=255)                                   caacaGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGaa                                                                                                   <  1:743888/140‑1 (MQ=255)                                       aGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGa                                                                                               >  2:275689/1‑140 (MQ=255)                                         aaTCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGaa                                                                                                   >  1:574367/1‑134 (MQ=255)                                         aaTCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGaa                                                                                                   <  2:574367/134‑1 (MQ=255)                                           tCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCaa                                                                                           >  1:348568/1‑140 (MQ=255)                                                    aaTTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTgg                                                                                  <  1:145649/140‑1 (MQ=255)                                                        attGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTGGCTGCGCTTTTTTAAAATTTAAAATTGATTAAACCTTTTGGCAGa                                                                              >  2:641226/1‑140 (MQ=255)                                                         ttGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGAt                                                                             <  1:52234/140‑1 (MQ=255)                                                          tGTTTCGTTGTTTTTGGTGGGATTCAGAAAATAAATATTTTAGACATTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTTGCTGGTGTTAATTAACAATTAAAAATTAATAATTCTTCTGGCAGAtt                                                                            >  2:833285/1‑140 (MQ=255)                                                            tttCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTaa                                                                          <  1:641226/140‑1 (MQ=255)                                                            tttCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTaa                                                                          <  2:491222/140‑1 (MQ=255)                                                              tCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTTGCTGCGCTTTTTTAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGc                                                                        >  2:527634/1‑140 (MQ=255)                                                                gttgttTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTTGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCa                                                                      >  2:393784/1‑140 (MQ=255)                                                                 ttgttTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCaa                                                                     >  1:129086/1‑140 (MQ=255)                                                                      tttGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTTGGTGCGCTTATTTAACAATTAGAAATGATCAATCCTTCTGGCAGATTAAGCCAAAATAc                                                                >  2:81976/1‑140 (MQ=255)                                                                              gATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAAt                                                                  <  1:384112/130‑1 (MQ=255)                                                                              gATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAAt                                                                  >  2:384112/1‑130 (MQ=255)                                                                               aTTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGc                                                       <  1:568863/140‑1 (MQ=255)                                                                                              atTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTAcg                                        <  1:238480/140‑1 (MQ=255)                                                                                               ttttAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTTGCTGCGCTTTTTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTAcgc                                       >  2:350080/1‑140 (MQ=255)                                                                                                  tAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGaa                                    <  2:348568/140‑1 (MQ=255)                                                                                                  tAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGaa                                    <  2:129086/140‑1 (MQ=255)                                                                                                         tAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTGGCTGCGCTTATTTAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCCTTCGGCAATGCTTCAGTTACGCGAAGAGGaaa                             >  2:820006/1‑139 (MQ=255)                                                                                                         tAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAAt                             <  1:927401/140‑1 (MQ=255)                                                                                                                   ggCGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTGGCTGCGCCTATTGAACATTTAAAATTGATTAATCCTTCTGGCAGATTAAACCAAAAAACCCGTTCGGCAATGCTTCAGTTACGCGGAGAGGAATGGGCTGAAtt                   >  2:210825/1‑140 (MQ=255)                                                                                                                     cGTTAAAAATGGGGCGGATAGCGAGGCGGTTTTTGCTGCGCTTTTTTAAAATTTTTAATTTATCAAACCTTCTGGGAGATTTAGCCAAAAAAACCCTTCGGCAAAGCTTCAGTTACCCGAAGAGGGATGGGCTGTATTCt                 >  2:714790/1‑140 (MQ=255)                                                                                                                               ggggCGGATAGCGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGtt       <  1:378148/140‑1 (MQ=255)                                                                                                                                 ggCGGATAGCGAGGCGGTTTTTGCTGCGCTTATTTAACATTTTGAATTGATCAATCCCTCTGGCAGATTAAGCCAAAAAAACCGTTCGGCAATGCTTTAGTTACGCGAAGAGGGATGGGCTGAATTTTTTTTTTTGTTGc     >  2:407281/1‑140 (MQ=255)                                                                                                                                  gCGGATAGCGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTCCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCt    <  1:18902/140‑1 (MQ=255)                                                                                                                                    ggATAGCGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTaa  <  1:479323/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      TTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAA  >  NC_000913/1245085‑1245355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑