Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,260,031	A→G	V258A (GTG→GCG)	dauA ←	aerobic C4‑dicarboxylate transporter DauA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,260,031	0	A	G	100.0%	155.2 / NA	44	V258A (GTG→GCG)	dauA	aerobic C4‑dicarboxylate transporter DauA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/23);  total (21/23)

ACCATCCAGCACCACGGCGCAGAGCAGAGATTCGATTGCGCCGAGCATTGCCATTGAGAATGCCGCAGGCAGCAGTGTGCGAATAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCACCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAAC  >  NC_000913/1259898‑1260157                                                                                                                                      |                                                                                                                               aCCATCCAGCACCACGGCGCAGAGCAGAGATTCGATTGCGCCGAGCATTGCCATTGAGAATGCCGCAGGCAGCAGTGTGCGAATAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGtt                                                                                                                          >  1:870871/1‑140 (MQ=255)      ccAGCACCACGGCGCAGAGCAGAGATTCGATTGCGCCGAGCATTGCCATTGAGAATGCCGCAGGCAGCAGTGTGCGAATAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGgc                                                                                                                     >  2:784353/1‑140 (MQ=255)          caccacGGCGCAGAGCAGAGATTCGATTGCGCCGAGCATTGCCATTGAGAATGCCGCAGGCAGCAGTGTGCGAATAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGgcagca                                                                                                                 >  2:577784/1‑140 (MQ=255)          caccacGGCGCAGAGCAGAGATTCGATTGCGCCGAGCATTGCCATTGAGAATGCCGCAGGCAGCAGTGTGCGAATAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGgcagca                                                                                                                 >  2:32044/1‑140 (MQ=255)          caccacGGCGCAGAGCAGAGATTCGATTGCGCCGAGCATTGCCATTGAGAATGCCGCAGGCAGCAGTGTGCGAATAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGgcagca                                                                                                                 >  2:120142/1‑140 (MQ=255)           accacGGCGCAGAGCAGAGATTCGATTGCGCCGAGCATTGCCATTGAGAATGCCGCAGGCAGCAGTGTGCGAATAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGcagcag                                                                                                                <  2:789852/140‑1 (MQ=255)              acGGCGCAGAGCAGAGATTCGATTGCGCCGAGCATTGCCATTGAGAATGCCGCAGGCAGCAGTGTGCGAATAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTg                                                                                                             <  2:354322/140‑1 (MQ=255)               cGGCGCAGAGCAGAGATTCGATTGCGCCGAGCATTGCCATTGAGAATGCCGCAGGCAGCAGTGTGCGAATAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGgcag                                                                                                                   <  1:237964/133‑1 (MQ=255)               cGGCGCAGAGCAGAGATTCGATTGCGCCGAGCATTGCCATTGAGAATGCCGCAGGCAGCAGTGTGCGAATAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGgcag                                                                                                                   >  2:523008/1‑133 (MQ=255)               cGGCGCAGAGCAGAGATTCGATTGCGCCGAGCATTGCCATTGAGAATGCCGCAGGCAGCAGTGTGCGAATAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGgcag                                                                                                                   <  1:523008/133‑1 (MQ=255)               cGGCGCAGAGCAGAGATTCGATTGCGCCGAGCATTGCCATTGAGAATGCCGCAGGCAGCAGTGTGCGAATAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGgcag                                                                                                                   >  2:237964/1‑133 (MQ=255)               cGGCGCAGAGCAGAGATTCGATTGCGCCGAGCATTGCCATTGAGAATGCCGCAGGCAGCAGTGTGCGAATAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGc                                                                                                            >  2:130006/1‑140 (MQ=255)                 gcgcAGAGCAGAGATTCGATTGCGCCGAGCATTGCCATTGAGAATGCCGCAGGCAGCAGTGTGCGAATAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCgg                                                                                                          <  1:130006/140‑1 (MQ=255)                   gcagagcagaGATTCGATTGCGCCGAGCATTGCCATTGAGAATGCCGCAGGCAGCAGTGTGCGAATAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGaa                                                                                                        <  1:577784/140‑1 (MQ=255)                         cagagATTCGATTGCGCCGAGCATTGCCATTGAGAATGCCGCAGGCAGCAGTGTGCGAATAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGAATACCGt                                                                                                  <  1:120142/140‑1 (MQ=255)                           gagaTTCGATTGCGCCGAGCATTGCCATTGAGAATGCCGCAGGCAGCAGTGTGCGAATAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGAATACCGTTa                                                                                                <  2:714086/140‑1 (MQ=255)                                        gcCGGGCATTGCCATTGAGAATGCCGCAGGCAGCAGTGTGCGAATAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCAt                                                                                   <  1:862116/140‑1 (MQ=255)                                         ccGAGCATTGCCATTGAGAATGCCGCAGGCAGCAGTGTGCGAATAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATc                                                                                  <  1:945190/140‑1 (MQ=255)                                         ccGAGCATTGCCATTGAGAATGCCGCAGGCAGCAGTGTGCGAATAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATc                                                                                  >  2:869110/1‑140 (MQ=255)                                              cATTGCCATTGAGAATGCCGCAGGCAGCAGTGTGCGAATAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCa                                                                             <  2:798423/140‑1 (MQ=255)                                              cATTGCCATTGAGAATGCCGCAGGCAGCAGTGTGCGAATAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCa                                                                             <  1:32044/140‑1 (MQ=255)                                                ttGCCATTGAGAATGCCGCAGGCAGCAGTGTGCGAATAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAgg                                                                           <  2:668103/140‑1 (MQ=255)                                                 tGCCATTGAGAATGCCGCAGGCAGCAGTGTGCGAATAGAATCCCAGGTTAGCGTGAATTCCGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAGGa                                                                          >  1:955179/1‑140 (MQ=255)                                                         agaATGCCGCAGGCAGCAGTGTGCGAATAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTgg                                                                  <  1:83496/140‑1 (MQ=255)                                                                   aggcagCAGTGTGCGAATAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAAc                                                        >  2:630271/1‑140 (MQ=255)                                                                              tgCGAATAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCa                                             >  2:414970/1‑140 (MQ=255)                                                                               gCGAATAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCaa                                            <  1:869110/140‑1 (MQ=255)                                                                                cGAATAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAAc                                           >  1:554797/1‑140 (MQ=255)                                                                                cGAATAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAAc                                           >  1:357053/1‑140 (MQ=255)                                                                                   aTAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGaa                                                         <  2:95821/123‑1 (MQ=255)                                                                                   aTAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGaa                                                         >  1:95821/1‑123 (MQ=255)                                                                                       aaTCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTccg                                    >  1:409934/1‑140 (MQ=255)                                                                                            cAGGTTAGCGTGAATTCCGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAg                               <  2:955179/140‑1 (MQ=255)                                                                                               gTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAg                            <  1:414970/140‑1 (MQ=255)                                                                                                 tAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGt                          >  1:103901/1‑140 (MQ=255)                                                                                                   gCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTa                        >  2:809775/1‑140 (MQ=255)                                                                                                    cGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTaa                       >  2:239882/1‑140 (MQ=255)                                                                                                    cGTGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTaa                       >  2:413225/1‑140 (MQ=255)                                                                                                      tGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACa                     <  1:809775/140‑1 (MQ=255)                                                                                                      tGAATTCTGAATTAGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACa                     <  2:870871/140‑1 (MQ=255)                                                                                                      tGAAGTCTGAATTAGGCAGATCACACGGCAGCGCCAGTTGCGGCAGGAGTTGCGGAGTACAGTTGCCCTGATAACCAACGGCCAGGACGTAGTGGAATTGAAAACCGATGGAAGCACCATGGCCGCCGAGCAGGTTAAcg                     <  2:409934/140‑2 (MQ=255)                                                                                                                   aGGCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACcg        <  2:357053/140‑1 (MQ=255)                                                                                                                    ggCAGATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACcgc       >  1:468832/1‑140 (MQ=255)                                                                                                                        gATCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCaa   <  2:468832/140‑1 (MQ=255)                                                                                                                         aTCCCACGGCAGCGCCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAAc  <  1:784353/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                               ACCATCCAGCACCACGGCGCAGAGCAGAGATTCGATTGCGCCGAGCATTGCCATTGAGAATGCCGCAGGCAGCAGTGTGCGAATAGAATCCCAGGTTAGCGTGAATTCTGAATTAGGCAGATCCCACGGCAGCACCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAAC  >  NC_000913/1259898‑1260157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑