Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,289,178	A→G	intergenic (‑46/‑67)	ychJ ← / → rssA	NTF2‑like domain‑containing protein YchJ/putative patatin‑like phospholipase RssA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,289,178	0	A	G	100.0%	172.7 / NA	49	intergenic (‑46/‑67)	ychJ/rssA	NTF2‑like domain‑containing protein YchJ/putative patatin‑like phospholipase RssA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/22);  total (27/22)

TGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGG  >  NC_000913/1289043‑1289309                                                                                                                                        |                                                                                                                                    tGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCAc                                                                                                                                 <  2:730489/140‑1 (MQ=255) tGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCAGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCAc                                                                                                                                 <  1:117243/140‑1 (MQ=255)   gATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGC‑ACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCa                                                                                                                                  >  2:749100/1‑136 (MQ=255)   gATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGC‑ACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCa                                                                                                                                  <  1:749100/136‑1 (MQ=255)      cAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGt                                                                                                                            >  2:349372/1‑140 (MQ=255)         gTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTaa                                                                                                                         >  1:284999/1‑140 (MQ=255)                   ttCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTa                                                                                                               >  1:543391/1‑140 (MQ=255)                                   gggTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGAt                                                                                               <  2:220310/140‑1 (MQ=255)                                     gTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTg                                                                                             <  1:231811/140‑1 (MQ=255)                                        gCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCAt                                                                                          >  1:302048/1‑140 (MQ=255)                                         cAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCAtt                                                                                         >  2:911021/1‑140 (MQ=255)                                         cAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCAtt                                                                                         >  1:410739/1‑140 (MQ=255)                                               aGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAggg                                                                                   <  2:396750/140‑1 (MQ=255)                                                 gCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGa                                                                                 >  1:638158/1‑140 (MQ=255)                                                      aCTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATc                                                                              <  1:35921/138‑1 (MQ=255)                                                      aCTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATc                                                                              >  2:35921/1‑138 (MQ=255)                                                      aCTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCtt                                                                            >  2:204/1‑140 (MQ=255)                                                       cTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTg                                                                           <  1:349372/140‑1 (MQ=255)                                                         cGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCg                                                                         <  2:543391/140‑1 (MQ=255)                                                          gACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCgg                                                                        >  2:170708/1‑140 (MQ=255)                                                           aCAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTg                                                                           <  2:898823/136‑1 (MQ=255)                                                           aCAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTg                                                                           >  1:898823/1‑136 (MQ=255)                                                            cAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGa                                                                      <  1:204/140‑1 (MQ=255)                                                               cACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGaata                                                                   >  1:686502/1‑140 (MQ=255)                                                                     cacaGGGGCAAAGCTGAGACACAAATAAGCTCCCTGGGAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCCTTCCAGGGGAATCTTGCGGGAATAATgaga                                                             <  2:284999/140‑1 (MQ=255)                                                                     cacaGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATgaga                                                             <  2:302048/140‑1 (MQ=255)                                                                     cacaGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATgaga                                                             <  2:884094/140‑1 (MQ=255)                                                                         gggACAAAGCTGAGACACAAATAATCTCCCTGGGAACAATAACGGCGTATTAACGGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGGGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGa                                                         >  2:436213/1‑140 (MQ=255)                                                                         gggACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCggg                                                                       >  1:852077/1‑126 (MQ=255)                                                                         gggACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCggg                                                                       <  2:852077/126‑1 (MQ=255)                                                                          ggACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGAt                                                        >  1:1164/1‑140 (MQ=255)                                                                            aCAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATaa                                                      >  1:265376/1‑140 (MQ=255)                                                                            aCAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATaa                                                      >  2:765405/1‑140 (MQ=255)                                                                              aaaGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATaaaa                                                    >  1:177996/1‑140 (MQ=255)                                                                                aGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAAt                                                  >  2:767285/1‑140 (MQ=255)                                                                                                   tcCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCt                               >  2:286556/1‑140 (MQ=255)                                                                                                     ccTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTgg                             >  1:702641/1‑140 (MQ=255)                                                                                                      cTGGAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTACATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGgc                            <  1:79452/140‑1 (MQ=255)                                                                                                         gAAACAATAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGcgcg                         <  2:638158/140‑1 (MQ=255)                                                                                                               aTAACGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCgaga                   >  2:285027/1‑140 (MQ=255)                                                                                                                  aCGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGt                <  1:286556/140‑1 (MQ=255)                                                                                                                   cGGCGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGtt               >  2:563552/1‑140 (MQ=255)                                                                                                                      cGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGt            <  2:686502/140‑1 (MQ=255)                                                                                                                      cGTATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGt            <  1:767285/140‑1 (MQ=255)                                                                                                                        tATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCgcggc                       >  1:234210/1‑127 (MQ=255)                                                                                                                        tATTAACCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCgcggc                       <  2:234210/127‑1 (MQ=255)                                                                                                                            aaCCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCata      <  1:170708/140‑1 (MQ=255)                                                                                                                             aCCGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCatat     >  2:330119/1‑140 (MQ=255)                                                                                                                               cGCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTg   >  2:125756/1‑140 (MQ=255)                                                                                                                                gCCTGAGTGGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTgg  <  1:563552/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    TGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGG  >  NC_000913/1289043‑1289309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑