Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,375,151	C→T	F63F (TTC→TTT)	ycjQ →	putative zinc‑binding dehydrogenase YcjQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,375,151	0	C	T	100.0%	210.0 / NA	60	F63F (TTC→TTT)	ycjQ	putative zinc‑binding dehydrogenase YcjQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/29);  total (31/29)

AAGATCGGGCGATTTTAGCTAATGAAGTGAAGATCCGCGTGCGTTTCGGCGCACCGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTCACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGTTGGCGACATTATCGAGTGCGGCAGCGACGTTACCGACTACGCGGTGGGCGACAGCGTATGCGGCTACG  >  NC_000913/1375015‑1375287                                                                                                                                         |                                                                                                                                         aaGATCGGGCGATTTTAGCTAATGAAGTGAAGATCCGCGTGCGTTTCGGCGCACCGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACt                                                                                                                                       >  1:90369/1‑140 (MQ=255) aaGATCGGGCGATTTTAGCTAATGAAGTGAAGATCCGCGTGCGTTTCGGCGCACCGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACt                                                                                                                                       >  1:331471/1‑140 (MQ=255) aaGATCGGGCGATTTTAGCTAATGAAGTGAAGATCCGCGTGCGTTTCGGCGCACCGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACt                                                                                                                                       >  1:783202/1‑140 (MQ=255)      cGGGCGATTTTAGCTAATGAAGTGAAGATCCGCGTGCGTTTCGGCGCACCGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCcgcg                                                                                                                                  >  1:407839/1‑140 (MQ=255)       gggCGATTTTAGCTAATGAAGTGAAGATCCGCGTGCGTTTCGGCGCACCGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGt                                                                                                                                 >  1:104619/1‑140 (MQ=255)       gggCGATTTTAGCTAATGAAGTGAAGATCCGCGTGCGTTTCGGCGCACCGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGt                                                                                                                                 >  2:212270/1‑140 (MQ=255)       gggCGATTTTAGCTAATGAAGTGAAGATCCGCGTGCGTTTCGGCGCACCGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGt                                                                                                                                 >  2:371881/1‑140 (MQ=255)                     aaTGAAGTGAAGATCCGCGTGCGTTTCGGCGCACCGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGcgcc                                                                                                                   <  1:212270/140‑1 (MQ=255)                        gaagtgaagATCCGCGTGCGTTTCGGCGCACCGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCcgcg                                                                                                                <  2:246691/140‑1 (MQ=255)                        gaagtgaagATCCGCGTGCGTTTCGGCGCACCGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCcgcg                                                                                                                <  1:371881/140‑1 (MQ=255)                        gaagtgaagATCCGCGTGCGTTTCGGCGCACCGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCcgcg                                                                                                                <  2:820947/140‑1 (MQ=255)                          agtgaagATCCGCGTGCGTTTCGGCGCACCGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCcgcgcg                                                                                                              >  2:958414/1‑140 (MQ=255)                             gaagATCCGCGTGCGTTTCGGCGCACCGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCcgcg                                                                                                                <  1:489136/135‑1 (MQ=255)                             gaagATCCGCGTGCGTTTCGGCGCACCGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCcgcg                                                                                                                >  2:489136/1‑135 (MQ=255)                             gaagATCCGCGTGCGTTTCGGCGCACCGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCa                                                                                                           <  1:656183/140‑1 (MQ=255)                                 aTCCGCGTGCGTTTCGGCGCACCGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGa                                                                                                       >  1:179563/1‑140 (MQ=255)                                   ccGCGTGCGTTTCGGCGCACCGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGt                                                                                                     <  2:104619/140‑1 (MQ=255)                                    cgcgTGCGTTTCGGCGCACCGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCa                                                                                                           <  1:726527/133‑1 (MQ=255)                                    cgcgTGCGTTTCGGCGCACCGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCa                                                                                                           >  2:726527/1‑133 (MQ=255)                                     gcgtgcgtTTCGGCGCACCGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGttt                                                                                                   >  2:362706/1‑140 (MQ=255)                                      cgtgcgtTTCGGCGCACCGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTg                                                                                                  >  2:287740/1‑140 (MQ=255)                                            tttCGGCGCACCGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAAt                                                                                            <  2:179563/140‑1 (MQ=255)                                                 gcgcACCGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAg                                                                                       <  2:878671/140‑1 (MQ=255)                                                  cgcACCGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGc                                                                                      <  2:90369/140‑1 (MQ=255)                                                   gcACCGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCt                                                                                     <  2:374235/140‑1 (MQ=255)                                                      ccGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTgg                                                                                  >  1:152041/1‑140 (MQ=255)                                                      ccGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTgg                                                                                  <  1:287740/140‑1 (MQ=255)                                                      ccGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTgg                                                                                  <  1:362706/140‑1 (MQ=255)                                                       cGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGc                                                                                 <  2:774164/140‑1 (MQ=255)                                                        gAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCa                                                                                <  1:958414/140‑1 (MQ=255)                                                                 aacggaaGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACAtggtgg                                                                       >  1:654549/1‑140 (MQ=255)                                                                   cggaaGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGtt                                                                     >  2:386929/1‑140 (MQ=255)                                                                     gaaGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGTTgg                                                                   <  1:386929/140‑1 (MQ=255)                                                                           gTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGtt                                                                     >  1:445170/1‑132 (MQ=255)                                                                           gTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGtt                                                                     <  2:445170/132‑1 (MQ=255)                                                                           gTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGTTGGCg                                                                 <  2:868067/136‑1 (MQ=255)                                                                           gTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGTTGGCg                                                                 >  1:868067/1‑136 (MQ=255)                                                                           gTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGTTGGCGACAt                                                             >  2:381028/1‑140 (MQ=255)                                                                           gTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGTTGGCGACAt                                                             >  2:650045/1‑140 (MQ=255)                                                                           gTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGTTGGCGACAt                                                             >  2:926988/1‑140 (MQ=255)                                                                                  tCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGTTGGCGACATTATCGAg                                                      >  2:926819/1‑140 (MQ=255)                                                                                     gcgcCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGTTGGCGACATTATCGAGTGc                                                   >  2:47451/1‑140 (MQ=255)                                                                                        ccgccAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGTTGGCGACATTATCGAGTGCGGc                                                <  2:325297/140‑1 (MQ=255)                                                                                         cgccAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGTTGGCGACATTATCGAGTGCGGCa                                               >  2:330734/1‑140 (MQ=255)                                                                                          gccAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGTTGGCGACATTATCGAGTGCGGCAg                                              <  2:165737/140‑1 (MQ=255)                                                                                                 cGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGTTGGCGACATTATCGAGTGCGGCAGCGACGtt                                       <  2:783202/140‑1 (MQ=255)                                                                                                    ttATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGTTGGCGACATTATCGAGTGCGGc                                                <  2:816848/128‑1 (MQ=255)                                                                                                    ttATTGATGAAGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGTTGGCGACATTATCGAGTGCGGc                                                >  1:816848/1‑128 (MQ=255)                                                                                                             aaGACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGTTGGCGACATTATCGAGTGCGGCAGCGACGTTACCGACTAcgcg                           >  2:930314/1‑140 (MQ=255)                                                                                                               gACTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGTTGGCGACATTATCGAGTGCGGCAGCGACGTTACCGACTACGCGGt                         <  1:330734/140‑1 (MQ=255)                                                                                                                 cTTTAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGTTGGCGACATTATCGAGTGCGGCAGCGACGTTACCGACTACGCGGTgg                       >  1:873818/1‑140 (MQ=255)                                                                                                                    tAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGTTGGCGACATTATCGAGTGCGGCAGCGACGTTACCGACTACGCGGTg                        <  2:605404/136‑1 (MQ=255)                                                                                                                    tAACGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGTTGGCGACATTATCGAGTGCGGCAGCGACGTTACCGACTACGCGGTg                        >  1:605404/1‑136 (MQ=255)                                                                                                                     aaCGGCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGTTGGCGACATTATCGAGTGCGGCAGCGACGTTACCGACTACGCGGTGGGCGa                   <  1:650045/140‑1 (MQ=255)                                                                                                                         gCGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGTTGGCGACATTATCGAGTGCGGCAGCGACGTTACCGACTACGCGGTGGGCGACAGc               >  2:674411/1‑140 (MQ=255)                                                                                                                          cGAATGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGTTGGCGACATTATCGAGTGCGGCAGCGACGTTACCGACTACGCGGTGGGCGACAGCg              >  1:418195/1‑140 (MQ=255)                                                                                                                            aaTGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGTTGGCGACATTATCGAGTGCGGCAGCGACGTTACCGACTACGCGGTGGGCGACAGCGTa            >  2:96668/1‑140 (MQ=255)                                                                                                                              tGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGTTGGCGACATTATCGAGTGCGGCAGCGACGTTACCGACTACGCGGTGGGCGACAGCGTATg          <  2:654549/140‑1 (MQ=255)                                                                                                                              tGGCAGATGTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGTTGGCGACATTATCGAGTGCGGCAGCGACGTTACCGACTACGCGGTGGGCGACAGCGTATg          <  2:873818/140‑1 (MQ=255)                                                                                                                                      gTTTACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGTTGGCGACATTATCGAGTGCGGCAGCGACGTTACCGACTACGCGGTGGGCGACAGCGTATGCGGCTACg  <  2:152041/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         AAGATCGGGCGATTTTAGCTAATGAAGTGAAGATCCGCGTGCGTTTCGGCGCACCGAAACACGGAACGGAAGTGGTCGACTTCCGCGCCGCCAGCCCGTTTATTGATGAAGACTTTAACGGCGAATGGCAGATGTTCACTCCGCGTCCGGCAGATGCGCCGCGCGGCATTGAGTTTGGCAAATTCCAGCTTGGCAACATGGTGGTTGGCGACATTATCGAGTGCGGCAGCGACGTTACCGACTACGCGGTGGGCGACAGCGTATGCGGCTACG  >  NC_000913/1375015‑1375287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑