Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	178,882	A→G	V91A (GTC→GCC)	mtn ←	5'‑methylthioadenosine/S‑adenosylhomocysteine nucleosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	178,882	0	A	G	100.0%	183.2 / NA	51	V91A (GTC→GCC)	mtn	5'‑methylthioadenosine/S‑adenosylhomocysteine nucleosidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/27);  total (24/27)

TTCGGCAATGCAGGCCTCAGCGGCAGCGATCAGTTTATCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAG  >  NC_000913/178749‑179018                                                                                                                                      |                                                                                                                                         ttCGGCAATGCAGGCCTCAGCGGCAGCGATCAGTTTATCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGa                                                                                                                                    >  1:361281/1‑140 (MQ=255)   cGGCAATGCAGGCCTCAGCGGCAGCGATCAGTTTATCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGata                                                                                                                                  >  2:41752/1‑140 (MQ=255)    ggCAATGCAGGCCTCAGCGGCAGCGATCAGTTTATCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGatat                                                                                                                                 <  2:845661/140‑1 (MQ=255)      cAATGCAGGCCTCAGCGGCAGCGATCAGTTTATCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCg                                                                                                                               <  2:99355/140‑1 (MQ=255)      cAATGCAGGCCTCAGCGGCAGCGATCAGTTTATCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCg                                                                                                                               <  1:38982/140‑1 (MQ=255)        aTGCAGGCCTCAGCGGCAGCGATCAGTTTATCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGcc                                                                                                                             <  1:742931/140‑1 (MQ=255)        aTGCAGGCCTCAGCGGCAGCGATCAGTTTATCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGcc                                                                                                                             <  1:774478/140‑1 (MQ=255)        aTGCAGGCCTCAGCGGCAGCGATCAGTTTATCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGcc                                                                                                                             <  1:455553/140‑1 (MQ=255)          gCAGGCCTCAGCGGCAGCGATCAGTTTATCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCa                                                                                                                           <  2:552038/140‑1 (MQ=255)           cAGGCCTCAGCGGCAGCGATCAGTTTATCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCAc                                                                                                                          >  2:269592/1‑140 (MQ=255)           cAGGCCTCAGCGGCAGCGATCAGTTTATCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCAc                                                                                                                          >  1:812609/1‑140 (MQ=255)                cTCAGCGGCAGCGATCAGTTTATCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCa                                                                                                                     >  2:210161/1‑140 (MQ=255)                     cGGCAGCGATCAGTTTATCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGtt                                                                                                                <  1:33097/140‑1 (MQ=255)                        cAGCGATCAGTTTATCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGt                                                                                                             >  2:319391/1‑140 (MQ=255)                          gCGATCAGTTTATCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGc                                                                                                           >  2:371231/1‑140 (MQ=255)                                     aTCGTCGGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCgg                                                                                                >  1:508650/1‑140 (MQ=255)                                            gCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAAcc                                                                                         <  1:77768/140‑1 (MQ=255)                                            gCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAAcc                                                                                         <  2:637002/140‑1 (MQ=255)                                            gCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAAcc                                                                                         >  1:627484/1‑140 (MQ=255)                                              tttAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCgg                                                                                       <  1:623474/140‑1 (MQ=255)                                                tAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCgg                                                                                       >  2:703978/1‑138 (MQ=255)                                                tAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCgg                                                                                       <  1:703978/138‑1 (MQ=255)                                                       tGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAAcc                                                                                         <  1:697526/129‑1 (MQ=255)                                                       tGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAAcc                                                                                         >  2:697526/1‑129 (MQ=255)                                                             aCAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACAt                                                                        <  1:319391/140‑1 (MQ=255)                                                             aCAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACAt                                                                        <  2:260380/140‑1 (MQ=255)                                                                 ccTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTaataat                                                                             >  1:561506/1‑131 (MQ=255)                                                                 ccTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTaataat                                                                             <  2:561506/131‑1 (MQ=255)                                                                      tAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGc                                                               <  1:210161/140‑1 (MQ=255)                                                                       aaCTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCa                                                              <  1:41752/140‑1 (MQ=255)                                                                           gACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGc                                                               <  2:260065/135‑1 (MQ=255)                                                                           gACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGc                                                               >  1:260065/1‑135 (MQ=255)                                                                           gACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAgtgt                                                          >  2:818010/1‑140 (MQ=255)                                                                           gACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAgtgt                                                          >  1:948533/1‑140 (MQ=255)                                                                           gACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAgtgt                                                          <  2:627484/140‑1 (MQ=255)                                                                                     aTAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCa                                                >  2:237576/1‑140 (MQ=255)                                                                                     aTAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCa                                                <  2:948533/140‑1 (MQ=255)                                                                                     aTAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCa                                                <  1:269592/140‑1 (MQ=255)                                                                                     aTAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCa                                                <  1:818010/140‑1 (MQ=255)                                                                                       aaCCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACAATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCaaa                                              >  2:138874/1‑140 (MQ=255)                                                                                          cAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTg                                           <  2:395903/140‑1 (MQ=255)                                                                                            aaTGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTg                                           >  2:96327/1‑138 (MQ=255)                                                                                            aaTGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTg                                           <  1:96327/138‑1 (MQ=255)                                                                                            aaTGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGc                                         >  1:213487/1‑140 (MQ=255)                                                                                                     aCATCCGCGTCGTGATAACGTGCTTCGTCCGAGGGAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGGTCCAACAGCAAAGTGGCACCCAgcgc                                >  2:851849/1‑140 (MQ=255)                                                                                                        tCCGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGcgccgc                             <  2:361281/140‑1 (MQ=255)                                                                                                         ccGCGTCGTGATAACGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGCGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCa                            >  1:238782/1‑140 (MQ=255)                                                                                                                      aCGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGa               <  1:851849/140‑1 (MQ=255)                                                                                                                       cGTGCTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGAt              >  1:95005/1‑140 (MQ=255)                                                                                                                           cTTCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGccc          >  1:373706/1‑140 (MQ=255)                                                                                                                            ttCGTCCGAGGCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCg         >  2:420950/1‑140 (MQ=255)                                                                                                                                   gaggCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAg  <  1:371231/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         TTCGGCAATGCAGGCCTCAGCGGCAGCGATCAGTTTATCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAG  >  NC_000913/178749‑179018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑