Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,517,056	A→G	intergenic (+54/+43)	patD → / ← yncL	gamma‑aminobutyraldehyde dehydrogenase/uncharacterized protein YncL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,517,056	0	A	G	100.0%	204.7 / NA	58	intergenic (+54/+43)	patD/yncL	gamma‑aminobutyraldehyde dehydrogenase/uncharacterized protein YncL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/23);  total (35/23)

AACTTTCTGGTTACGGCAAGGATATGTCACTTTATGGGCTGGAGGATTACACCGTCGTCCGCCACGTCATGGTTAAACATTAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCACTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAGAGATAAGAGTAAACAAACCAACAGCAGCAAAGAAATTTATCAGTACTACAGTTCTACTGGAAACAT  >  NC_000913/1516920‑1517190                                                                                                                                         |                                                                                                                                       aaCTTTCTGGTTACGGCAAGGATATGTCACTTTATGGGCTGGAGGATTACACCGTCGTCCGCCACGTCATGGTTAAACATTAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTg                                                                                                                                     <  1:193899/140‑1 (MQ=255) aaCTTTCTGGTTACGGCAAGGATATGTCACTTTATGGGCTGGAGGATTACACCGTCGTCCGCCACGTCATGGTTAAACATTAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTg                                                                                                                                     <  1:495115/140‑1 (MQ=255) aaCTTTCTGGTTACGGCAAGGATATGTCACTTTATGGGCTGGAGGATTACACCGTCGTCCGCCACGTCATGGTTAAACATTAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTg                                                                                                                                     <  1:388333/140‑1 (MQ=255)     ttCTGGTTACGGCAAGGATATGTCACTTTATGGGCTGGAGGATTACACCGTCGTCCGCCACGTCATGGTTAAACATTAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGAttt                                                                                                                                 <  2:873774/140‑1 (MQ=255)                               tttATGGGCTGGAGGATTACACCGTCGTCCGCCACGTCATGGTTAAACATTAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCaaa                                                                                                       >  2:19100/1‑140 (MQ=255)                               tttATGGGCTGGAGGATTACACCGTCGTCCGCCACGTCATGGTTAAACATTAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCaaa                                                                                                       >  1:232271/1‑140 (MQ=255)                               tttATGGGCTGGAGGATTACACCGTCGTCCGCCACGTCATGGTTAAACATTAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCaaa                                                                                                       >  1:242991/1‑140 (MQ=255)                               tttATGGGCTGGAGGATTACACCGTCGCCCGCCACGTCATGGTTAAACATTCAGCATAATATTGCAGACCGTAAGAGTATGAGATGATCTTGCGTACTGCCAGAACGCTGATTTCTCTGCCAGTACGCACTGCGTGCaaa                                                                                                       >  2:862769/1‑140 (MQ=255)                                     ggCTGGAGGATTACACCGTCGTCCGCCACGTCATGGTTAAACATTAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACt                                                                                                 >  2:457322/1‑140 (MQ=255)                                     ggCTGGAGGATTACACCGTCGTCCGCCACGTCATGGTTAAACATTAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACt                                                                                                 >  1:598829/1‑140 (MQ=255)                                       cTGGAGGATTACACCGTCGTCCGCCACGTCATGGTTAAACATTAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTAc                                                                                               <  2:232271/140‑1 (MQ=255)                                        tGGAGGATTACACCGTCGTCCGCCACGTCATGGTTAAACATTAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACa                                                                                              <  1:212152/140‑1 (MQ=255)                                        tGGAGGATTACACCGTCGTCCGCCACGTCATGGTTAAACATTAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACa                                                                                              <  1:805739/140‑1 (MQ=255)                                          gaggaTTACACCGTCGTCCGCCACGTCATGGTTAAACATTAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATc                                                                                            >  2:846716/1‑140 (MQ=255)                                           aggaTTACACCGTCGTCCGCCACGTCATGGTTAAACATTAAGGATAATATTGCAGATCGTGAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACa                                                                                              >  2:673166/1‑137 (MQ=255)                                           aggaTTACACCGTCGTCCGCCACGTCATGGTTAAACATTAAGGATAATATTGCAGATCGTGAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACa                                                                                              <  1:673166/137‑1 (MQ=255)                                           aggaTTACACCGTCGTCCGCCACGTCATGGTTAAACATTAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCa                                                                                           >  1:711280/1‑140 (MQ=255)                                                tACACCGTCGTCCGCCACGTCATGGTTAAACATTAAGGATAATATTGCAGATCGTAGGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATaa                                                                                      >  1:923157/1‑140 (MQ=255)                                                 acacCGTCGTCCGCCACGTCATGGTTAAACATTAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATaaa                                                                                     <  1:862769/140‑1 (MQ=255)                                                  cacCGTCGTCCGCCACGTCATGGTTAAACATTAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAAc                                                                                    >  1:386785/1‑140 (MQ=255)                                                      gtcgtcCGCCACGTCATGGTTATACATTAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGc                                                                                >  1:45887/1‑140 (MQ=255)                                                       tcgtcCGCCATGTCATGGTTAAACATTAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGcc                                                                               <  2:711280/140‑1 (MQ=255)                                                       tcgtcCGCCACGTCATGGTTAAACATTAAGGATAATATTGCAGATCGTAGGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGcc                                                                               <  2:923157/140‑1 (MQ=255)                                                       tcgtcCGCCACGTCATGGTTAAACATTAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGcc                                                                               >  2:601075/1‑140 (MQ=255)                                                            cGCCACGTCATGGTTATACATTAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAAtc                                                                          <  2:45887/140‑1 (MQ=255)                                                                 cGTCATGGTTAAACATTAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCaa                                                                             >  2:551227/1‑132 (MQ=255)                                                                 cGTCATGGTTAAACATTAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCaa                                                                             <  1:551227/132‑1 (MQ=255)                                                                     aTGGTTAAACATTAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAgaga                                                                 <  1:690876/140‑1 (MQ=255)                                                                     aTGGTTAAACATTAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAgaga                                                                 <  2:685493/140‑1 (MQ=255)                                                                     aTGGTTAAACATTAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAgaga                                                                 <  2:211080/140‑1 (MQ=255)                                                                            aaCATTAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAGAGATAAGAGt                                                          >  1:954645/1‑140 (MQ=255)                                                                               aTTAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAGAGATAAGAGTaaa                                                       <  2:920565/140‑1 (MQ=255)                                                                                 tAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAgaga                                                                 >  1:645032/1‑128 (MQ=255)                                                                                 tAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAgaga                                                                 <  2:645032/128‑1 (MQ=255)                                                                                 tAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAGAGATAAGAGTaaaca                                                     >  2:60817/1‑140 (MQ=255)                                                                                 tAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAGAGATAAGAGTaaaca                                                     >  1:655833/1‑140 (MQ=255)                                                                                       taataTTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAGAGATAAGAGTAAACAAACCaa                                               <  2:954645/140‑1 (MQ=255)                                                                                               cAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAGAGATAAGAGTAAACAAACCAACAgcagca                                       >  1:213790/1‑140 (MQ=255)                                                                                                aGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAGAGATAAGAGTAAACAAACCAACAGCAGCaa                                      <  2:242991/140‑1 (MQ=255)                                                                                                    cGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAGAGATAAGAGTAAACAAACCAACAGCAGCAAAGaa                                  >  1:953333/1‑140 (MQ=255)                                                                                                            tATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAGAGATAAGAGTAAACAAACCAACAGCAGCAAAGAAAttt                              <  2:869696/136‑1 (MQ=255)                                                                                                            tATGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAGAGATAAGAGTAAACAAACCAACAGCAGCAAAGAAAttt                              >  1:869696/1‑136 (MQ=255)                                                                                                              tGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAGAGATAAGAGTAAACAAACCAACAGCAGCAAAGAAATTTATCAGt                        >  2:550226/1‑140 (MQ=255)                                                                                                              tGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAGAGATAAGAGTAAACAAACCAACAGCAGCAAAGAAATTTATCAGt                        >  1:301836/1‑140 (MQ=255)                                                                                                              tGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAGAGATAAGAGTAAACAAACCAACAGCAGCAAAGAAATTTATCAGt                        >  1:571072/1‑140 (MQ=255)                                                                                                              tGAGATGATCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAGAGATAAGAGTAAACAAACCAACAGCAGCAAAGAAATTTATCAGt                        >  1:953394/1‑140 (MQ=255)                                                                                                                 gatgatCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAGAGATAAGAGTAAACAAACCAACAGCAGCAAAGAAATTTATCAGtact                     >  1:360002/1‑140 (MQ=255)                                                                                                                    gatCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAGAGATAAGAGTAAACAAACCAACAGCAGCAAAGAAATTTATCAGTACTACa                  >  1:44643/1‑140 (MQ=255)                                                                                                                    gatCTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAGAGATAAGAGTAAACAAACCAACAGCAGCAAAGAAATTTATCAGTACTACa                  >  1:242616/1‑140 (MQ=255)                                                                                                                       cTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAGAGATAAGAGTAAACAAACCAACAGCAGCAAAGAAATTTATCAGTACTACAGtt               <  2:571072/140‑1 (MQ=255)                                                                                                                       cTTGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAGAGATAAGAGTAAACAAACCAACAGCAGCAAAGAAATTTATCAGTACTACAGtt               <  1:19100/140‑1 (MQ=255)                                                                                                                         tGCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCATATAAACCAGCCAAATCTCATAGAGATAAGAGTAAACAAACCAACAGCAGCAAAGAAATTTATCAGT‑CTACAGTTCTa            >  2:260909/1‑140 (MQ=255)                                                                                                                          gCGTACTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAGAGATAAGAGTAAACAAACCAACAGCAGCAAAGAAATTTATCAGTACTACAGTTCTa            >  2:899277/1‑140 (MQ=255)                                                                                                                               cTGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAGAGATAAGAGTAAACAAACCAACAGCAGCAAAGAAATTTATCAGTACTACAGTTCTACTGGa       >  2:294185/1‑140 (MQ=255)                                                                                                                                tGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAGAGATAAGAGTAAACAAACCAACAGCAGCAAAGAAATTTATCAGTACTACAGTTCTACTGGaa      >  1:149513/1‑140 (MQ=255)                                                                                                                                tGTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAGAGATAAGAGTAAACAAACCAACAGCAGCAAAGAAATTTATCAGTACTACAGTTCTACTGGaa      >  2:553457/1‑140 (MQ=255)                                                                                                                                 gTCAGAGCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAGAGATAAGAGTAAACAAACCAACAGCAGCAAAGAAATTTATCAGTACTACAGTTCTACTGGaaa     >  2:392489/1‑140 (MQ=255)                                                                                                                                    agagCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAGAGATAAGAGTAAACAAACCAACAGCAGCAAAGAAATTTATCAGTACTACAGTTCTACTGGAAAcat  >  1:54137/1‑140 (MQ=255)                                                                                                                                    agagCGCTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAGAGATAAGAGTAAACAAACCAACAGCAGCAAAGAAATTTATCAGTACTACAGTTCTACTGGAAAcat  >  1:436844/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       AACTTTCTGGTTACGGCAAGGATATGTCACTTTATGGGCTGGAGGATTACACCGTCGTCCGCCACGTCATGGTTAAACATTAAGGATAATATTGCAGATCGTAAGAGTATGAGATGATCTTGCGTACTGTCAGAGCACTGATTTCTCTGACAGTACGTGCTGCGTGCAAAATCACTACATCAAATAAACCAGCCAAATCTCATAGAGATAAGAGTAAACAAACCAACAGCAGCAAAGAAATTTATCAGTACTACAGTTCTACTGGAAACAT  >  NC_000913/1516920‑1517190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑