Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,604,757 T→C R237R (CGT→CGC)  lsrD → Autoinducer‑2 ABC transporter membrane subunit LsrD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,604,7570TC100.0% 112.2 / NA 34R237R (CGT→CGC) lsrDAutoinducer‑2 ABC transporter membrane subunit LsrD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/11);  total (23/11)

GCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAA  >  NC_000913/1604628‑1604886
                                                                                                                                 |                                                                                                                                 
gCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCg                                                                                                                         <  2:836536/140‑1 (MQ=255)
        cgcgcgTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGcgtcg                                                                                                                 <  2:844316/140‑1 (MQ=255)
          cgcgTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGAtc                                                                                                                            >  2:454832/1‑127 (MQ=255)
          cgcgTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGAtc                                                                                                                            <  1:454832/127‑1 (MQ=255)
                           cgcgATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCa                                                                                              >  1:289465/1‑140 (MQ=255)
                            gcgATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCAc                                                                                             <  2:289465/140‑1 (MQ=255)
                              gATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGCATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCAccg                                                                                           >  2:785766/1‑140 (MQ=255)
                                ttCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCAccgcc                                                                                         <  2:582107/140‑1 (MQ=255)
                                 tCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCAccgccg                                                                                        <  1:108669/140‑1 (MQ=255)
                                 tCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCAccgccg                                                                                        <  2:527450/140‑1 (MQ=255)
                                 tCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCAccgccg                                                                                        <  1:19531/140‑1 (MQ=255)
                                 tCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGCATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCAccgccg                                                                                        <  1:785766/140‑1 (MQ=255)
                                 tCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCGGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCAccgccg                                                                                        <  1:649483/140‑1 (MQ=255)
                                          ccGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTg                                                                               >  1:405355/1‑140 (MQ=255)
                                          ccGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTg                                                                               >  1:323426/1‑140 (MQ=255)
                                                   aTGTGCGCTCTATGCAATGACGGGGCTGGCGTCTGCGGTGGCCGTTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTAATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCa                                                                      <  2:135795/140‑1 (MQ=255)
                                                    tgtgCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCaa                                                                     >  1:345123/1‑140 (MQ=255)
                                                    tgtgCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCaa                                                                     >  1:609867/1‑140 (MQ=255)
                                                     gtgCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAat                                                                    >  1:135795/1‑140 (MQ=255)
                                                     gtgCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAat                                                                    >  1:435570/1‑140 (MQ=255)
                                                      tgCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAata                                                                   >  1:925112/1‑140 (MQ=255)
                                                        cgcTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATAtt                                                                 >  1:175484/1‑140 (MQ=255)
                                                              aTGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTAtggt                                                           <  2:405355/140‑1 (MQ=255)
                                                                   aTGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTggttc                                                      <  1:931371/140‑1 (MQ=255)
                                                                     gACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTggttccg                                                    >  2:358094/1‑140 (MQ=255)
                                                                     gACGGGGCTGGCGTCTGCGGTCGCCACTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTggttccg                                                    >  1:500245/1‑140 (MQ=255)
                                                                       cGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTggttccggt                                                  >  1:267895/1‑140 (MQ=255)
                                                                        ggggCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTggttccggtt                                                 <  2:323426/140‑1 (MQ=255)
                                                                         gggCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTggttccggttc                                                >  2:501839/1‑140 (MQ=255)
                                                                         gggCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTggttccggttc                                                >  2:235261/1‑140 (MQ=255)
                                                                          ggCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTggttc                                                      >  1:640019/1‑133 (MQ=255)
                                                                          ggCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTggttc                                                      <  2:640019/133‑1 (MQ=255)
                                                                           gCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTggttccggttccg                                              >  1:499866/1‑139 (MQ=255)
                                                                           gCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCa                                              >  1:555803/1‑140 (MQ=255)
                                                                           gCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCa                                              >  1:101508/1‑140 (MQ=255)
                                                                                    tGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCa                                     >  1:531874/1‑140 (MQ=255)
                                                                                         tCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGcc                                >  2:340148/1‑140 (MQ=255)
                                                                                                             tCGTTTTTTGTTTCAGCACGCTCGGTTCTGGGGGCGTCTTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTggg            <  2:609867/140‑1 (MQ=255)
                                                                                                                       gTTCAGCACGCTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGcaa  >  1:962006/1‑140 (MQ=255)
                                                                                                                                 |                                                                                                                                 
GCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAA  >  NC_000913/1604628‑1604886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑