Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	184,069	A→G	G9G (GGT→GGC)	yaeH ←	DUF3461 domain‑containing protein YaeH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	184,069	0	A	G	100.0%	160.9 / NA	45	G9G (GGT→GGC)	yaeH	DUF3461 domain‑containing protein YaeH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/26);  total (19/26)

CGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATACCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTT  >  NC_000913/183935‑184197                                                                                                                                       |                                                                                                                                 cGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATGCCCAg                                                                                                                             <  2:129468/140‑1 (MQ=255) cGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATGCCCAg                                                                                                                             >  1:875079/1‑140 (MQ=255)    tGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGATTATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATGCCCAGACt                                                                                                                          <  2:875079/140‑1 (MQ=255)           gATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATGCCCAGACTTTTCAGa                                                                                                                   <  1:27278/140‑1 (MQ=255)           gATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATGCCCAGACTTTTCAGa                                                                                                                   <  2:344690/140‑1 (MQ=255)           gATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATGCCCAGACTTTTCAGa                                                                                                                   <  2:24011/140‑1 (MQ=255)               tttAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATGCCCAGACTTTTCAGATTGt                                                                                                               <  2:798702/140‑1 (MQ=255)                ttAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATGCCCAGACTTTTCAGATTGTc                                                                                                              >  2:179066/1‑140 (MQ=255)                        aaCGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATGCCCAGACTTTTCAGATTGt                                                                                                               >  2:185718/1‑131 (MQ=255)                        aaCGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATGCCCAGACTTTTCAGATTGt                                                                                                               <  1:185718/131‑1 (MQ=255)                        aaCGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATGCCCAGACTTTTCAGATTGTCGTACATAg                                                                                                      >  1:602682/1‑140 (MQ=255)                        aaCGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATGCCCAGACTTTTCAGATTGTCGTACATAg                                                                                                      >  1:759895/1‑140 (MQ=255)                           gCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATGCCCAGACTTTTCAGATTGTCGTACATAGCGt                                                                                                   <  1:769103/140‑1 (MQ=255)                                     aaaaaCTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCaaaa                                                                                         <  1:659598/140‑1 (MQ=255)                                                tttGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTaa                                                                              >  2:54007/1‑140 (MQ=255)                                                 ttGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTaaa                                                                             >  2:453299/1‑140 (MQ=255)                                                              aaTAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTAtttt                                                                <  1:453299/140‑1 (MQ=255)                                                                   aTTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCAt                                                           <  2:759895/140‑1 (MQ=255)                                                                   aTTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCAt                                                           <  2:93601/140‑1 (MQ=255)                                                                      ttCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTtg                                                        >  2:558442/1‑140 (MQ=255)                                                                       tCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGATAATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTtgg                                                       >  2:666863/1‑140 (MQ=255)                                                                        cAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTtggc                                                      <  2:268410/140‑1 (MQ=255)                                                                            atatCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTtggcctgg                                                  <  1:558442/140‑1 (MQ=255)                                                                                cGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGgcccg                                              >  2:561665/1‑140 (MQ=255)                                                                                  ttgttgGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGgcccgcc                                            >  1:847302/1‑140 (MQ=255)                                                                                     ttgGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCgg                                         <  1:179066/140‑1 (MQ=255)                                                                                     ttgGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGCTAATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCgg                                         >  2:661627/1‑140 (MQ=255)                                                                                       ggCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCt                                       >  2:854121/1‑140 (MQ=255)                                                                                       ggCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATGCCCAGACTTTTCAGATTGTCGTACATAGCATTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCt                                       <  2:602682/140‑1 (MQ=255)                                                                                            ccTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCg                                     >  2:395206/1‑137 (MQ=255)                                                                                            ccTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCg                                     <  1:395206/137‑1 (MQ=255)                                                                                                          tAACGATCAATTTCTTCAGGATTGGTAATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGa                              <  1:724533/130‑1 (MQ=255)                                                                                                          tAACGATCAATTTCTTCAGGATTGGTAATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGa                              >  2:724533/1‑130 (MQ=255)                                                                                                          tAACGATCAATTTCTTCAGGATTGGTAATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAg                    <  2:847302/140‑1 (MQ=255)                                                                                                               aTCAATTTCTTCAGGATTGGTAATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCtgt               >  2:891299/1‑138 (MQ=255)                                                                                                                     ttcttcAGGATTGGTAATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTttcttc         <  1:935314/140‑1 (MQ=255)                                                                                                                      tcttcAGGATTGGTAATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTcc        <  2:866073/140‑1 (MQ=255)                                                                                                                       cttcAGGATTGGTAATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCa       <  1:788711/140‑1 (MQ=255)                                                                                                                       cttcAGGATTGGTAATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCa       <  1:891299/140‑1 (MQ=255)                                                                                                                          cAGGATTGGTCATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGAc    <  1:661627/140‑1 (MQ=255)                                                                                                                          cAGGATTGGTAATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCa       <  1:172956/137‑1 (MQ=255)                                                                                                                          cAGGATTGGTAATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCa       >  2:172956/1‑137 (MQ=255)                                                                                                                            ggATTGGTAATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACtt  >  2:548889/1‑140 (MQ=255)                                                                                                                                  gTAATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCa       >  1:392680/1‑129 (MQ=255)                                                                                                                                  gTAATGCCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCa       <  2:392680/129‑1 (MQ=255)                                                                                                                                       |                                                                                                                                 CGCTGACGCGGATATTTAAACTTAACGCTCTTGGCGAAAAACTCGCCTTTGTCTTTCTGGAAATAGATTTTCAGAATATCGTTGTTGGCTTCCTGCCGGAGGCTGTAACGATCAATTTCTTCAGGATTGGTAATACCCAGACTTTTCAGATTGTCGTACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTT  >  NC_000913/183935‑184197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑