Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,656,072	G→A	A89T (GCC→ACC)	ynfB →	DUF1283 domain‑containing protein YnfB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,656,072	0	G	A	100.0%	177.3 / NA	52	A89T (GCC→ACC)	ynfB	DUF1283 domain‑containing protein YnfB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/27);  total (25/27)

GCCGCCAGCACGCCGCTATGGAAAAAGAGCAATGGAATGACACGCGCAATCTGCGCCAGAAAGTGAATAAACGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCGCCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGTTATTACCCCCTAACCTGTTATTGATTTAAGGAATGTAAGGACACGTTATGCCAAGCGCCCA  >  NC_000913/1655935‑1656197                                                                                                                                          |                                                                                                                              gccgccAGCACGCCGCTATGGAAAAAGAGCAATGGAATGACACGCGCAATCTGCGCCAGAAAGTGAATAAACGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCAcc                                                                                                                             <  2:330580/140‑1 (MQ=255)   cgccAGCACGCCGCTATGGAAAAAGAGCAATGGAATGACACGCGCAATCTGCGCCAGAAAGTGAATAAACGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCaa                                                                                                                           >  2:378052/1‑140 (MQ=255)   cgccAGCACGCCGCTATGGAAAAAGAGCAATGGAATGACACGCGCAATCTGCGCCAGAAAGTGAATAAACGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCaa                                                                                                                           <  1:716092/140‑1 (MQ=255)   cgccAGCACGCCGCTATGGAAAAAGAGCAATGGAATGACACGCGCAATCTGCGCCAGAAAGTGAATAAACGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCaa                                                                                                                           >  1:251234/1‑140 (MQ=255)        gCACGCCGCTATGGAAAAAGAGCAATGGAATGACACGCGCAATCTGCGCCAGAAAGTGAATAAACGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCa                                                                                                                      >  2:2969/1‑140 (MQ=255)         cACGCCGCTATGGAAAAAGAGCAATGGAATGACACACGCAATCTGCGCCAGAAAGTGAATAAACGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCaa                                                                                                                     <  2:664821/140‑1 (MQ=255)                cTATGGAAAAAGAGCAATGGAATGACACGCGCAATCTGCGCCAGAAAGTGAATAAACGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTAc                                                                                                              >  1:658032/1‑140 (MQ=255)                  aTGGAAAAAGAGCAATGGAATGACACGCGCAATCTGCGCCAGAAAGTGAATAAACGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTg                                                                                                            >  2:793850/1‑140 (MQ=255)                   tGGAAAAAGAGCAATGGAATGACACGCGCAATCTGCGCCAGAAAGTGAATAAACGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTgg                                                                                                           >  2:565525/1‑140 (MQ=255)                   tGGAAAAAGAGCAATGGAATGACACGCGCAATCTGCGCCAGAAAGTGAATAAACGCACTGAAAAAGAGTGAGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTgg                                                                                                           >  1:321770/1‑140 (MQ=255)                          agagCAATGGAATGACACGCGCAATCTGCGCCAGAAAGTGAATAAACGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCa                                                                                                    >  2:792967/1‑140 (MQ=255)                                    aaTGACACGCGCAATCTGCGCCAGAAAGTGAATAAACGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTgcg                                                                                          <  1:101394/140‑1 (MQ=255)                                    aaTGACACGCGCAATCTGCGCCAGAAAGTGAATAAACGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTgcg                                                                                          <  1:793850/140‑1 (MQ=255)                                           cgcgCAATCTGCGCCAGAAAGTGAATAAACGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTgcg                                                                                          >  1:1871/1‑133 (MQ=255)                                           cgcgCAATCTGCGCCAGAAAGTGAATAAACGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTgcg                                                                                          <  2:1871/133‑1 (MQ=255)                                           cgcgCAATCTGCGCCAGAAAGTGAATAAACGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTg                                                                                   <  1:439324/140‑1 (MQ=255)                                             cgcAATCTGCGCCAGAAAGTGAATAAATGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGa                                                                                 >  1:943144/1‑140 (MQ=255)                                             cgcAATCTGCGCCAGAAAGTGAATAAACGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGa                                                                                 >  2:341778/1‑140 (MQ=255)                                              gcAATCTGCGCCAGAAAGTGAATAAACGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGAc                                                                                >  2:409116/1‑140 (MQ=255)                                                  tCTGCGCCAGAAAGTGAATAAACGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTgc                                                                                           >  1:541860/1‑125 (MQ=255)                                                  tCTGCGCCAGAAAGTGAATAAACGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTgc                                                                                           <  2:541860/125‑1 (MQ=255)                                                    tGCGCCAGAAAGTGAATAAACGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGa                                                                          >  2:387906/1‑140 (MQ=255)                                                          aGAAAGTGAATAAACGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGc                                                                    <  1:792967/140‑1 (MQ=255)                                                            aaaGTGAATAAACGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCcg                                                                  <  2:567768/140‑1 (MQ=255)                                                                   aTAAACGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATTCTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGttatt                                                           <  2:658032/140‑1 (MQ=255)                                                                   aTAAACGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGttatt                                                           <  1:2969/140‑1 (MQ=255)                                                                   aTAAACGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGttatt                                                           <  1:565525/140‑1 (MQ=255)                                                                   aTAAACGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGttatt                                                           <  2:639992/140‑1 (MQ=255)                                                                    tAAACGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGttatta                                                          <  2:738303/140‑1 (MQ=255)                                                                        cGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCcgc                                                                 <  1:217939/129‑1 (MQ=255)                                                                        cGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCcgc                                                                 >  2:217939/1‑129 (MQ=255)                                                                         gCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATCCTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGTTATTAccccc                                                     <  2:596715/140‑1 (MQ=255)                                                                          cACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGTTATTACCCCCt                                                    >  1:273230/1‑140 (MQ=255)                                                                           aCTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGTTATTACCCCCTa                                                   <  1:341778/140‑1 (MQ=255)                                                                            cTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGttatt                                                           >  1:37512/1‑131 (MQ=255)                                                                            cTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGttatt                                                           <  2:37512/131‑1 (MQ=255)                                                                               aaaaaGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGTTATTACCCCCTAACCt                                               >  2:422526/1‑140 (MQ=255)                                                                                     agTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGTTATTACCCCCTAACCTGTTa                                           <  2:881501/138‑1 (MQ=255)                                                                                     agTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGTTATTACCCCCTAACCTGTTa                                           >  1:881501/1‑138 (MQ=255)                                                                                             aaaGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGTTATTACCCCCTAACCTGTTATTGATTTAAg                                 <  1:409116/140‑1 (MQ=255)                                                                                             aaaGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGTTATTACCCCCTAACCTGTTATTGATTTAAg                                 <  1:120210/140‑1 (MQ=255)                                                                                              aaGCCGACGCCGCTTTCGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGTTATTACCCCCTAACCTGTTATTGATTTAAgg                                <  2:862251/140‑1 (MQ=255)                                                                                                    aCGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCTAACTGGCCGCGTTATTACCCCCTAACCTGTTATTGATTTAAGGAATGTa                          >  2:326605/1‑140 (MQ=255)                                                                                                    aCGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGTTATTACCCCCTAACCTGTTATTGATTTAAg                                 <  1:826847/133‑1 (MQ=255)                                                                                                    aCGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGTTATTACCCCCTAACCTGTTATTGATTTAAg                                 >  2:826847/1‑133 (MQ=255)                                                                                                            tttGATAACCGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGTTATTACCCCCTAACCTGTTATTGATTTAAGGAATGTAAGGACACg                  <  1:134103/140‑1 (MQ=255)                                                                                                                    ccGCGATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGTTATTACCCCCTAACCTGTTATTGATTTAAGGAATGTAAGGACACGTTATGCCa          >  2:148243/1‑140 (MQ=255)                                                                                                                     cgcgATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGTTATTACCCCCTAACCTGTTATTGATTTAAGGAATGTAAGGACACGTTATGCCaa         >  1:350097/1‑140 (MQ=255)                                                                                                                      gcgATAAATGTGAGCGAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGTTATTACCCCCTAACCTGTTATTGATTTAAGGAATGTa                          <  1:68616/122‑1 (MQ=255)                                                                                                                      gcgATAAATGTGAGCGAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGTTATTACCCCCTAACCTGTTATTGATTTAAGGAATGTa                          >  2:68616/1‑122 (MQ=255)                                                                                                                       cgATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGTTATTACCCCCTAACCTGTTATTGATTTAAGGAATGTAAGGa                      >  2:414283/1‑125 (MQ=255)                                                                                                                       cgATAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGTTATTACCCCCTAACCTGTTATTGATTTAAGGAATGTAAGGa                      <  1:414283/125‑1 (MQ=255)                                                                                                                          tAAATGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGTTATTACCCCCTAACCTGTTATTGATTTAAGGAATGTAAGGACACGTTATGCCAAGCGcc    >  2:230363/1‑140 (MQ=255)                                                                                                                            aaTGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGTTATTACCCCCTAACCTGTTATTGATTTAAGGAATGTAAGGACACGTTATGCCAAGCGCCca  <  2:350097/140‑1 (MQ=255)                                                                                                                            aaTGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGTTATTACCCCCTAACCTGTTATTGATTTAAGGAATGTAAGGACACGTTATGCCAAGCGCCca  <  1:387906/140‑1 (MQ=255)                                                                                                                            aaTGTGAGCAAAGCACCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGTTATTACCCCCTAACCTGTTATTGATTTAAGGAATGTAAGGACACGTTATGCCAAGCGCCca  <  1:326605/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                              GCCGCCAGCACGCCGCTATGGAAAAAGAGCAATGGAATGACACGCGCAATCTGCGCCAGAAAGTGAATAAACGCACTGAAAAAGAGTGGGATAAAGCCGACGCCGCTTTTGATAACCGCGATAAATGTGAGCAAAGCGCCAACATCAATGCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGTTATTACCCCCTAACCTGTTATTGATTTAAGGAATGTAAGGACACGTTATGCCAAGCGCCCA  >  NC_000913/1655935‑1656197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑