Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,695,427	T→C	E215E (GAA→GAG)	uidA ←	beta‑glucuronidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,695,427	0	T	C	100.0%	178.7 / NA	51	E215E (GAA→GAG)	uidA	beta‑glucuronidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/20);  total (31/20)

TGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGC  >  NC_000913/1695292‑1695557                                                                                                                                        |                                                                                                                                   tgtctgGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCaa                                                                                                                                >  1:285091/1‑140 (MQ=255)   tctgGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACg                                                                                                                              >  2:701040/1‑140 (MQ=255)   tctgGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACg                                                                                                                              >  1:834318/1‑140 (MQ=255)   tctgGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGAGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACg                                                                                                                              >  2:665565/1‑140 (MQ=255)       gCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGa                                                                                                                          >  2:120413/1‑140 (MQ=255)         ttttGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACa                                                                                                                        >  1:125745/1‑140 (MQ=255)             ggCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCAc                                                                                                                    >  2:708194/1‑140 (MQ=255)                tgtgACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCAt                                                                                                                 <  1:120413/140‑1 (MQ=255)                 gtgACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCAtt                                                                                                                >  1:867325/1‑140 (MQ=255)                        acaGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGccacc                                                                                                         <  2:890534/140‑1 (MQ=255)                              cATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGc                                                                                                   <  2:867325/140‑1 (MQ=255)                              cATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGc                                                                                                   <  1:701040/140‑1 (MQ=255)                                    gaTAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCa                                                                                             >  2:811827/1‑140 (MQ=255)                                    gaTAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCa                                                                                             >  1:129477/1‑140 (MQ=255)                                    gaTAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCa                                                                                             >  2:264610/1‑140 (MQ=255)                                     aTAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCaa                                                                                            <  2:129477/140‑1 (MQ=255)                                      tAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCaccacc                                                                                                      >  2:110223/1‑129 (MQ=255)                                      tAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCaccacc                                                                                                      <  1:110223/129‑1 (MQ=255)                                      tAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAAc                                                                                           >  2:707638/1‑140 (MQ=255)                                            tCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGAcgc                                                                                     >  1:18617/1‑140 (MQ=255)                                            tCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGAcgc                                                                                     <  2:285091/140‑1 (MQ=255)                                             cACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGAcgcg                                                                                    <  1:665565/140‑1 (MQ=255)                                             cACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGAcgcg                                                                                    <  1:317010/140‑1 (MQ=255)                                               cccGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGa                                                                                        <  1:712801/134‑1 (MQ=255)                                               cccGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGa                                                                                        >  2:712801/1‑134 (MQ=255)                                               cccGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTg                                                                                  >  1:232504/1‑140 (MQ=255)                                                ccGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTgg                                                                                 >  2:778958/1‑140 (MQ=255)                                                   gTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTa                                                                              <  1:811827/140‑1 (MQ=255)                                                    ttGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCTTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTAc                                                                             >  1:724229/1‑140 (MQ=255)                                                             gTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTgcg                                                                    <  1:707638/140‑1 (MQ=255)                                                              tGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTgcgc                                                                   >  1:384106/1‑140 (MQ=255)                                                              tGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTgcgc                                                                   >  1:606450/1‑140 (MQ=255)                                                              tGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTgcgc                                                                   >  1:738754/1‑140 (MQ=255)                                                                cGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGa                                                                 >  1:630756/1‑140 (MQ=255)                                                                 ggATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGAc                                                                >  1:847205/1‑140 (MQ=255)                                                                       accacTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGt                                                          <  2:834318/140‑1 (MQ=255)                                                                       accacTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGt                                                          <  1:459209/140‑1 (MQ=255)                                                                       accacTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGt                                                          >  2:370815/1‑140 (MQ=255)                                                                            ttGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTcacca                                                     <  2:232504/140‑1 (MQ=255)                                                                             tGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTcaccac                                                    >  2:527367/1‑140 (MQ=255)                                                                                      ccGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCCACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATc                                           <  2:837266/140‑1 (MQ=255)                                                                                             tGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCAcc                                    <  2:606450/140‑1 (MQ=255)                                                                                                   gTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGgtg                              >  1:799295/1‑140 (MQ=255)                                                                                                     ccAGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGtt                            <  2:630756/140‑1 (MQ=255)                                                                                                       aGTTGCAACCACCTGTTGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCg                          <  1:778958/140‑1 (MQ=255)                                                                                                                      ttGATCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCa           <  2:125745/140‑1 (MQ=255)                                                                                                                         aTCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTa        >  1:841261/1‑140 (MQ=255)                                                                                                                          tCCGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTAc       >  1:240519/1‑140 (MQ=255)                                                                                                                           ccGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACg      <  2:847205/140‑1 (MQ=255)                                                                                                                            cGCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGc     >  2:487210/1‑140 (MQ=255)                                                                                                                             gCATCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCt    >  2:848943/1‑140 (MQ=255)                                                                                                                               aTCACGCAGCTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGc  >  1:8051/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                   TGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGC  >  NC_000913/1695292‑1695557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑