Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,715,617	T→C	E91E (GAA→GAG)	pdxY ←	pyridoxal kinase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,715,617	0	T	C	100.0%	157.1 / NA	46	E91E (GAA→GAG)	pdxY	pyridoxal kinase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/23);  total (23/23)

TGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATGACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGTTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGT  >  NC_000913/1715486‑1715750                                                                                                                                    |                                                                                                                                      tGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATGACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCa                                                                                                                                    <  2:65061/135‑1 (MQ=255) tGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATGACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCa                                                                                                                                    >  1:65061/1‑135 (MQ=255)   ccGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATGACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACCCTGCt                                                                                                                             >  2:781464/1‑140 (MQ=255)   ccGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTATTTCCGGATGACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACCCTGCt                                                                                                                             >  1:507037/1‑140 (MQ=255)        caTGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATGACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACCCTGCTccgcc                                                                                                                        >  1:149287/1‑140 (MQ=255)        caTGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATGACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACCCTGCTccgcc                                                                                                                        >  2:772550/1‑140 (MQ=255)           gAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATGACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACCCTGCTCCGCCGAt                                                                                                                     <  1:561703/140‑1 (MQ=255)                  tGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATGACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGAt                                                                                                              >  1:79626/1‑140 (MQ=255)                  tGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATGACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGAt                                                                                                              <  1:278662/140‑1 (MQ=255)                   gCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATGACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATa                                                                                                             >  2:920569/1‑140 (MQ=255)                   gCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATGACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATa                                                                                                             >  1:584985/1‑140 (MQ=255)                    cGACACCCGTTGCAACGATACAGCCTTTTTCCGGATGACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACTCTGCTCCGCCGATCCCAGATAg                                                                                                            <  1:952613/140‑1 (MQ=255)                    cGACACCCGGTGCAACGATACAGTCTTTTTCCGGATGACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATAg                                                                                                            >  2:841143/1‑140 (MQ=255)                    cGACACCCGGTGCAACGATACAGCCTTTTTCCGGATGACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCCCCCTGCTCCGCCGATCCCAGATAg                                                                                                            <  1:884455/140‑1 (MQ=255)                    cGACACCCGGTGCAACGATACAGCCTTTTTCCGGATGACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATAg                                                                                                            <  2:755971/140‑1 (MQ=255)                    cGACACCCGGTGCAACGATACAGCCTATTTCCGGATGACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATAg                                                                                                            <  2:507037/140‑1 (MQ=255)                          ccGGTGCAACGATACAGCCTTTTTCCGGATGACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATAGCCACtt                                                                                                      >  1:776287/1‑140 (MQ=255)                               gCAACGATACAGCCTTTTTCCGGATGACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATAc                                                                                                 <  2:149287/140‑1 (MQ=255)                                    gATACAGTCTTTTTCCGGATGACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCAt                                                                                            <  1:841143/140‑1 (MQ=255)                                    gATACAGCCTTTTTCCGGATGACCCATTCCCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACCCTCCTCCGCCGATCCCAGATAGCCACTTAATACGGCAt                                                                                            <  2:91304/140‑1 (MQ=255)                                    gATACAGCCTTTTTCCGGATGACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGTACGATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCAt                                                                                            <  2:801065/140‑1 (MQ=255)                                    gATACAGCCTTTTTCCGGATGACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCAt                                                                                            <  1:277492/140‑1 (MQ=255)                                      tACAGCCTTTTTCCGGATGACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATca                                                                                          <  1:772550/140‑1 (MQ=255)                                      tACAGCATTTTTCCGGATGACCCATTCCCGTATCGCAAAAATATTTCGCCGGCGGATCCGCGGCTTTCCCCTGACGGACGATACCGAGGATATGCTCCCCCTGTTCCGCCGATCCCAGATAGCCACTTAATACGGCATca                                                                                          <  2:826686/140‑1 (MQ=255)                                           ccTTTTTCCGGATGACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGgt                                                                                     >  2:818776/1‑140 (MQ=255)                                                     gATGACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTa                                                                           >  2:718708/1‑140 (MQ=255)                                                          cccATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAAt                                                                      <  1:920569/140‑1 (MQ=255)                                                               tACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATggcgg                                                                 >  1:168045/1‑140 (MQ=255)                                                                     aTCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGc                                                           >  1:271831/1‑140 (MQ=255)                                                                         cAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTg                                                       >  1:720478/1‑140 (MQ=255)                                                                         cAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTg                                                       >  2:576046/1‑140 (MQ=255)                                                                                  ttCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTc                                              <  2:168045/140‑1 (MQ=255)                                                                                       cTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTa                                         >  1:154370/1‑140 (MQ=255)                                                                                        tGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTaa                                        <  2:778736/140‑1 (MQ=255)                                                                                         gCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTaaa                                       >  1:830942/1‑140 (MQ=255)                                                                                            gATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATgg                                    <  2:830942/140‑1 (MQ=255)                                                                                                          cccagacggacgAGACCAAGGATATGCCCCCCCGGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATccc                      <  2:720478/136‑3 (MQ=255)                                                                                                          cccTGACGGACGATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCAc                      <  1:134044/139‑1 (MQ=255)                                                                                                           ccTGACGGACGATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACg                     <  2:271831/140‑1 (MQ=255)                                                                                                               acggacgATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTggg                               >  2:621083/1‑126 (MQ=255)                                                                                                               acggacgATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTggg                               <  1:621083/126‑1 (MQ=255)                                                                                                                cggacgATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGcc                <  1:576046/140‑1 (MQ=255)                                                                                                                   acgATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGc                    <  1:610452/133‑1 (MQ=255)                                                                                                                   acgATACCGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGc                    >  2:610452/1‑133 (MQ=255)                                                                                                                         ccGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCAttt       >  2:277194/1‑140 (MQ=255)                                                                                                                          cGAGGATATGCTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTg      <  1:495468/140‑1 (MQ=255)                                                                                                                            aGGATATGCTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGcc    >  1:902356/1‑140 (MQ=255)                                                                                                                            aGGATATGCTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGcc    <  1:718708/140‑1 (MQ=255)                                                                                                                              gATATGCTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGt  >  2:44221/1‑140 (MQ=255)                                                                                                                                 atGCTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGcc    <  1:722161/135‑1 (MQ=255)                                                                                                                                    |                                                                                                                                      TGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATGACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGTTCACCCTGCTCCGCCGATCCCAGATAGCCACTTAATACGGCATCACAGGTGTGTAATTTATCAATGGCGGCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGT  >  NC_000913/1715486‑1715750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑