Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,728,437	G→A	A31T (GCC→ACC)	rnt →	RNase T

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,728,437	0	G	A	100.0%	167.0 / NA	49	A31T (GCC→ACC)	rnt	RNase T
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/20);  total (29/20)

TTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGA  >  NC_000913/1728302‑1728564                                                                                                                                        |                                                                                                                                tttGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCaa                                                                                                                             <  2:420058/140‑1 (MQ=255) tttGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCaa                                                                                                                             <  1:309515/140‑1 (MQ=255)     tCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAAcc                                                                                                                         >  2:719106/1‑140 (MQ=255)     tCATAATGCGCGCTGCAATTGATCCGTATTAAGAGAATCAGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAAcc                                                                                                                         >  2:48237/1‑140 (MQ=255)           tGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATgcg                                                                                                                   <  1:797632/140‑1 (MQ=255)               cgcTGCAATTTATCCGTATTAAGAGAATCAGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATTGATGTTGAAACAGCCGGATTTAACACCaaaa                                                                                                                           <  2:153572/128‑1 (MQ=255)               cgcTGCAATTTATCCGTATTAAGAGAATCAGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATTGATGTTGAAACAGCCGGATTTAACACCaaaa                                                                                                                           >  1:153572/1‑128 (MQ=255)                gcTGCAATTTATCCGTATTAAGAGAATCAGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCgctgct                                                                                                              <  2:232707/140‑1 (MQ=255)                gcTGCAATTTATCCGTATTAAGAGAATCAGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCgctgct                                                                                                              <  1:387359/140‑1 (MQ=255)                              gTATTAAGAGAATCAGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCa                                                                                                >  1:65123/1‑140 (MQ=255)                               tATTAAGAGAATCAGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCAt                                                                                               >  2:700539/1‑140 (MQ=255)                                         aTCAGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCAt                                                                                               >  2:673391/1‑130 (MQ=255)                                         aTCAGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCAt                                                                                               <  1:673391/130‑1 (MQ=255)                                          tCAGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCAt                                                                                               >  2:910633/1‑129 (MQ=255)                                          tCAGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCAt                                                                                               <  1:910633/129‑1 (MQ=255)                                          tCAGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGaaaa                                                                                    >  1:577787/1‑140 (MQ=255)                                          tCAGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGaaaa                                                                                    >  1:535691/1‑140 (MQ=255)                                          tCAGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGaaaa                                                                                    >  1:52397/1‑140 (MQ=255)                                            aGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATg                                                                                  <  2:805055/140‑1 (MQ=255)                                                  ccGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCa                                                                                             <  1:542037/123‑1 (MQ=255)                                                  ccGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCa                                                                                             >  2:542037/1‑123 (MQ=255)                                                   cGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAAc                                                                           >  1:664295/1‑140 (MQ=255)                                                           cTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAggctgg                                                                   >  1:25953/1‑140 (MQ=255)                                                           cTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAggctgg                                                                   >  2:719773/1‑140 (MQ=255)                                                                 ttACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATg                                                             <  1:720157/140‑1 (MQ=255)                                                                    ccGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCg                                                          <  2:535691/140‑1 (MQ=255)                                                                    ccGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCg                                                          <  2:577787/140‑1 (MQ=255)                                                                          tgtgCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGAcacc                                                    >  1:759592/1‑140 (MQ=255)                                                                           gtgCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGAcacca                                                   >  2:693766/1‑140 (MQ=255)                                                                            tgCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGAcaccac                                                  >  1:316443/1‑140 (MQ=255)                                                                            tgCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGAcaccac                                                  >  1:797733/1‑140 (MQ=255)                                                                            tgCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGAcaccac                                                  >  1:479562/1‑140 (MQ=255)                                                                             gCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACcaca                                                 <  1:700539/140‑1 (MQ=255)                                                                              cGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCacat                                                <  1:719106/140‑1 (MQ=255)                                                                                 ccGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCacattac                                             <  1:693766/140‑1 (MQ=255)                                                                                          tGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACg                                    <  1:43596/140‑1 (MQ=255)                                                                                                     cTGTTGTGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCAttt                         >  2:131724/1‑140 (MQ=255)                                                                                                       gttgtGATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTATATTTCCACGTCGAACCATTTGt                       >  1:747058/1‑140 (MQ=255)                                                                                                         tgtgATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGt                                   <  2:491829/126‑1 (MQ=255)                                                                                                         tgtgATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGt                                   >  1:491829/1‑126 (MQ=255)                                                                                                           tgATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGGTGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGgc                   >  2:175383/1‑140 (MQ=255)                                                                                                           tgATCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGgc                   >  1:633599/1‑140 (MQ=255)                                                                                                             aTCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTc                                  <  2:401020/123‑1 (MQ=255)                                                                                                             aTCGATGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTc                                  >  1:401020/1‑123 (MQ=255)                                                                                                                  tGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAAtt            >  1:215987/1‑140 (MQ=255)                                                                                                                  tGTTGAAACAGCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCAAACCATTTGTCGGCGCAAAtt            >  1:289924/1‑140 (MQ=255)                                                                                                                      gAAACAGCCGGATTTAACACCAAAACCGGTGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCa        <  1:175383/140‑1 (MQ=255)                                                                                                                            gCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGa  >  2:308067/1‑140 (MQ=255)                                                                                                                            gCCGGATTTAACACCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGa  >  2:825245/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                TTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGA  >  NC_000913/1728302‑1728564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑