Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	213,556	T→C	V409A (GTA→GCA)	tilS →	tRNA(Ile)‑lysidine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	213,556	0	T	C	100.0%	145.5 / NA	43	V409A (GTA→GCA)	tilS	tRNA(Ile)‑lysidine synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/22);  total (21/22)

ATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCT  >  NC_000913/213422‑213689                                                                                                                                       |                                                                                                                                      aTTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTtg                                                                                                                                  <  1:200028/140‑1 (MQ=255)  ttGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTtgt                                                                                                                                 <  1:892358/140‑1 (MQ=255)         atatTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCaa                                                                                                                          <  2:936594/140‑1 (MQ=255)          tatTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCaag                                                                                                                         >  1:95875/1‑140 (MQ=255)               tCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGgt                                                                                                                    <  2:853146/140‑1 (MQ=255)               tCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGgt                                                                                                                    <  2:15103/140‑1 (MQ=255)                  ggCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGtgtg                                                                                                                 >  2:935072/1‑140 (MQ=255)                  ggCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGGTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGtgtg                                                                                                                 >  2:419511/1‑140 (MQ=255)                    cGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCaaga                                                                                                                        >  2:831895/1‑131 (MQ=255)                    cGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCaaga                                                                                                                        <  1:831895/131‑1 (MQ=255)                    cGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGc                                                                                                               <  1:140549/140‑1 (MQ=255)                     gTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGCt                                                                                                              <  1:935072/140‑1 (MQ=255)                       aaCGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGCTGa                                                                                                            >  2:877933/1‑140 (MQ=255)                        aCGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGCTGaa                                                                                                           <  2:95875/140‑1 (MQ=255)                              gACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGGTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGCTGAAGGTgag                                                                                                     <  1:419511/140‑1 (MQ=255)                                      cTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGt                                                                                             >  2:918980/1‑140 (MQ=255)                                                      aaGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAg                                                                             >  2:150552/1‑140 (MQ=255)                                                              ggCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACg                                                                       >  2:610004/1‑138 (MQ=255)                                                              ggCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACg                                                                       <  1:610004/138‑1 (MQ=255)                                                              ggCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCt                                                                     >  1:936931/1‑140 (MQ=255)                                                              ggCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCt                                                                     <  1:578683/140‑1 (MQ=255)                                                                  tGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGt                                                                 <  2:936931/140‑1 (MQ=255)                                                                         gTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGa                                                          <  1:150552/140‑1 (MQ=255)                                                                            gCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGCT‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCt                                                                     <  2:689068/125‑1 (MQ=255)                                                                            gCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGCT‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCt                                                                     >  1:689068/1‑125 (MQ=255)                                                                             cTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGGGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGc                                                      >  2:811077/1‑140 (MQ=255)                                                                                  ggACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGAt                                                 <  1:156788/139‑1 (MQ=255)                                                                                        cacGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACg                                           >  2:433464/1‑140 (MQ=255)                                                                                          cGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCTGGGGCATTTGCGACGCAAGTTGGAGTCGCTGGAGGGCGGGATGGCGTAATTTTTCTCTGGCAGAAAACGCGTAGTTCAGTGGAAGCCGGATAAGGCGCa                                         >  2:714278/1‑140 (MQ=255)                                                                                          cGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGGATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCa                                         >  1:670274/1‑140 (MQ=255)                                                                                              aCTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCaa                                     <  1:877933/140‑1 (MQ=255)                                                                                               ctgctgTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAg                                              <  2:502791/130‑1 (MQ=255)                                                                                               ctgctgTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAg                                              >  1:502791/1‑130 (MQ=255)                                                                                               ctgctgTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCaaa                                    >  2:57951/1‑140 (MQ=255)                                                                                                tgctgTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAAc                                   >  2:741608/1‑140 (MQ=255)                                                                                                  ctgTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGt                                 >  2:833735/1‑140 (MQ=255)                                                                                                      tttATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCa                             >  1:419534/1‑140 (MQ=255)                                                                                                       ttATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCAt                            >  1:480866/1‑140 (MQ=255)                                                                                                       ttATGGCGAAACGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCAt                            >  1:384336/1‑140 (MQ=255)                                                                                                               aaaCGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGaa                    >  1:696267/1‑140 (MQ=255)                                                                                                                  cGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGt                 <  2:384336/140‑1 (MQ=255)                                                                                                                  cGCTGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGt                 <  2:480866/140‑1 (MQ=255)                                                                                                                     tGATTGCGGCGGCAGGGGCATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCaa              <  1:433464/140‑1 (MQ=255)                                                                                                                               ggcAGGGGCATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCg    <  2:696267/140‑1 (MQ=255)                                                                                                                                 cAGGGGCATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCt  >  1:933247/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                      ATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCT  >  NC_000913/213422‑213689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑