Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,749,625	T→C	T242A (ACC→GCC)	ydhU ←	putative cytochrome YdhU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,749,625	0	T	C	100.0%	133.5 / NA	38	T242A (ACC→GCC)	ydhU	putative cytochrome YdhU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/18);  total (20/18)

TGGCGCAGGAACCAGCGATACATCACCGCTCCGATACGCCATTCTCGCAGGTCTGCTTGTGTAATGATCATTCGTTAGTGCCGGTGATAGCCATCGACCATGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGTGCAAAGATAAAGATGACCGAAGATAAAAAAGAGGCTTATAAATGCCAGAGCAAAATGTGTCTGCAATAACCAGTATCTTACGCCAGGAAACACATCTCCCACGGCTTGCGGATAGAGACACAGCAGC  >  NC_000913/1749489‑1749752                                                                                                                                         |                                                                                                                                tGGCGCAGGAACCAGCGATACATCACCGCTCCGATACGCCATTCTCGCAGGTCTGCTTGTGTAATGATCATTCGTTAGTGCCGGTGATAGCCATCGACCATGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCa                                                                                                                              <  2:152493/140‑1 (MQ=255)  ggCGCAGGAACCAGCGATACATCACCGCTCCGATACGCCATTCTCGCAGGTCTGCTTGTGTAATGATCATTCGTTAGTGCCGGTGATAGCCATCGACCATGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCaa                                                                                                                             <  1:476742/140‑1 (MQ=255)              aGCGATACATCACCGCTCCGATACGCCATTCTCGCAGGTCTGCTTGTGTAATGATCATTCGTTAGTGCCGGTGATAGCCATCGACCATGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGa                                                                                                                 >  1:25726/1‑140 (MQ=255)                 gATACATCACCGCTCCGATACGCCATTCTCGCAGGTCTGCTTGTGTAATGATCATTCGTTAGTGCCGGTGATAGCCATCGACCATGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCg                                                                                                              <  1:134715/140‑1 (MQ=255)                     cATCACCGCTCCGATACGCCATTCTCGCAGGTCTGCTTGTGTAATGATCATTCGTTAGTGCCGGTGATAGCCATCGACCATGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGa                                                                                                          >  2:596071/1‑140 (MQ=255)                      aTCACCGCTCCGATACGCCATTCTCGCAGGTCTGCTTGTGTAATGATCATTCGTTAGTGCCGGTGATAGCCATCGACCATGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGAt                                                                                                         <  2:920103/140‑1 (MQ=255)                       tCACCGCTCCGATACGCCATTCTCGCAGGTCTGCTTGTGTAATGATCATTCGTTAGTGCCGGTGATAGCCATCGACCATGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATa                                                                                                        >  2:845545/1‑140 (MQ=255)                            gCTCCGATACGCCATTCTCGCAGGTCTGCTTGTGTAATGATCATTCGTTAGTGCCGGTGATAGCCATCGACCATGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATaaaaaa                                                                                                   <  2:25726/140‑1 (MQ=255)                            gCTCCGATACGCCATTCTCGCAGGTCTGCTTGTGTAATGATCATTCGTTAGTGCCGGTGATAGCCATCGACCATGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATaaaaaa                                                                                                   <  2:13840/140‑1 (MQ=255)                             cTCCGATACGCCATTCTCGCAGGTCTGCTTGTGTAATGATCATTCGTTAGTGCCGGTGATAGCCATCGACCATGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATAAAAAag                                                                                                  >  2:824028/1‑140 (MQ=255)                             cTCCGATACGCCATTCTCGCAGGTCTGCTTGTGTAATGATCATTCGTTAGTGCCGGTGATAGCCATCGACCATGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATAAAAAag                                                                                                  >  2:576714/1‑140 (MQ=255)                                 gATACGCCATTCTCGCAGGTCTGCTTGTGTAATGATCATTCGTTAGTGCCGGTGATAGCCATCGACCATGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATAAAAAAGAGGc                                                                                              <  2:772108/140‑1 (MQ=255)                                            ctcGCAGGTCTGCTTGTGTAATGATCATTCGTTAGTGCCGGTGATAGCCATCGACCATGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATAAAAAAGAGGCTTATAAATGcc                                                                                   <  1:596071/140‑1 (MQ=255)                                                cAGGTCTGCTTGTGTAATGATCATTCGTTAGTGCCGGTGATAGCCATCGACCATGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATAAAAAAGAGGCTTATAAATGCCagag                                                                               >  1:590447/1‑140 (MQ=255)                                                cAGGTCTGCTTGTGTAATGATCATTCGTTAGTGCCGGTGATAGCCATCGACCATGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATAAAAAAGAGGCTTATAAATGCCagag                                                                               >  2:803340/1‑140 (MQ=255)                                                     cTGCTTGTGTAATGATCATTCGTTAGTGCCGGTGATAGCCATCGACCATGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATAAAAAAGAGGCTTATAAATGCCAGAGCaaaa                                                                          <  1:520056/140‑1 (MQ=255)                                                      tGCTTGTGTAATGATCATTCGTTAGTGCCGGTGATAGCCATCGACCATGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATAAAAAAGAGGCTTATAAATGCCAGAGCAAAAt                                                                         >  2:892507/1‑140 (MQ=255)                                                      tGCTTGTGTAATGATCATTCGTTAGTGCCGGTGATAGCCATCGACCATGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATAAAAAAGAGGCTTATAAATGCCAGAGCAAAAt                                                                         >  2:596382/1‑140 (MQ=255)                                                      tGCTTGTGTAATGATCATTCGTTAGTGCCGGTGATAGCCATCGACCATGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATAAAAAAGAGGCTTATAAATGCCAGAGCAAAAt                                                                         >  2:102623/1‑140 (MQ=255)                                                             gtAATGATCATTCGTTAGTGCCGGTGATAGCCATCGACCATGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATAAAAAAGAGGCTTATAAATGCCAGAGCAAAATGTGTCTg                                                                  >  1:885529/1‑140 (MQ=255)                                                               aaTGATCATTCGTTAGTGCCGGTGATAGCCATCGACCATGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATAAAAAAGAGGCTTATAAATGCCAGAGCAAAATGTGTCTGCa                                                                <  1:824028/140‑1 (MQ=255)                                                               aaTGATCATTCGTTAGTGCCGGTGATAGCCATCGACCATGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATAAAAAAGAGGCTTATAAATGCCAGAGCAAAATGTGTCTGCa                                                                <  1:102623/140‑1 (MQ=255)                                                                      aTTCGTTAGTGCCGGTGATAGCCATCGACCATGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATAAAAAAGAGGCTTATAAATGCCAGAGCAAAATGTGTCTGCAATAACCa                                                         >  2:326684/1‑140 (MQ=255)                                                                         cGTTAGTGCCGGTGATAGCCATCGACCATGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATAAAAAAGAGGCTTATAAATGCCAGAGCAAAATGTGTCTGCAATAACCAGTa                                                      >  2:214560/1‑140 (MQ=255)                                                                                        tAGCCATCGACCATGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATAAAAAAGAGGCTTATAAATGCCAGAGCAAAATGTGTCTGCAATAACCAGTATCTTACGCCAGGaaa                                       >  2:872597/1‑140 (MQ=255)                                                                                            cATCGACCATGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATAAAAAAGAGGCTTATAAATGCCAGAGCAAAATGTGTCTGCAATAACCAGTATCTTACGCCAGGAAAcaca                                   <  2:590447/140‑1 (MQ=255)                                                                                            cATCGACCATGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATAAAAAAGAGGCTTATAAATGCCAGAGCAAAATGTGTCTGCAATAACCAGTATCTTACGCCAGGAAAcaca                                   <  1:326684/140‑1 (MQ=255)                                                                                               cGACCATGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATAAAAAAGAGGCTTATAAATGCCAGAGCAAAATGTGTCTGCAATAACCAGTATCTTACGCCAGGAAACACAtct                                >  2:816051/1‑140 (MQ=255)                                                                                                     tGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATAAAAAAGATGCTTATAAATGCCAGAGCAAAATGTGTCTGCAATAACCAGTATCTTACGCCAGGAAACACATCTCCCACg                          >  2:727056/1‑140 (MQ=255)                                                                                                          ttAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATAAAAAAGAGGCTTATAAATGCCAGAGCAAAATGTGTCTGCAATAACCAGTATCTTACGCCAGGAAACACATCTCCCACGGCTTg                     <  2:404961/140‑1 (MQ=255)                                                                                                          ttAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATAAAAAAGAGGCTTATAAATGCCAGAGCAAAATGTGTCTGCAATAACCAGTATCTTACGCCAGGAAACACATCTCCCACGGCTTg                     <  1:214560/140‑1 (MQ=255)                                                                                                           tAAAGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATAAAAAAGAGGCTTATAAATGCCAGAGCAAAATGTGTCTGCAATAACCAGTATCTTACGCCAGGAAACACATCTCCCACGGCTTGc                    >  1:779619/1‑140 (MQ=255)                                                                                                            aaaGGTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATAAAAAAGAGGCTTATAAATGCCAGAGCAAAATGTGTCTGCAATAACCAGTATCTTACGCCAGGAAACACATCTCCCACGGCTTGCg                   <  1:256123/140‑1 (MQ=255)                                                                                                                gTTTCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATAAAAAAGATGCTTATAAATGCCAGAGCAAAATGTGTCTGCAATAACCAGTATCTTACGCCAGGAAACACATCTCCCACGGCTTGCGGATa               <  1:727056/140‑1 (MQ=255)                                                                                                                 tttCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATAAAAAAGAGGCTTATAAATGCCAGAGCAAAATGTGTCTGCAATAACCAGTATCTTACGCCAGGAAACACATCTCCCACGGCTTGCGGATag              >  1:727044/1‑140 (MQ=255)                                                                                                                  ttCGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATAAAAAAGAGGCTTATAAATGCCAGAGCAAAATGTGTCTGCAATAACCAGTATCTTACGCCAGGAAACACATCTCCCACGGCTTGCGGATaga             >  1:675105/1‑140 (MQ=255)                                                                                                                    cGTGTGGCGTACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATAAAAAAGAGGCTTATAAATGCCAGAGCAAAATGTGTCTGCAATAACCAGTATCTTACGCCAGGAAACACATCTCCCACGGCTTGCGGATAgaga           <  1:892507/140‑1 (MQ=255)                                                                                                                             tACGCCCCGTGGCGCAAAGATAAAGATGACCGAAGATAAAAAAGAGGCTTATAAATGCCAGAGCAAAATGTGTCTGCAATAACCAGTATCTTACGCCAGGAAACACATCTCCCACGGCTTGCGGATAGAGACACagcagc  >  2:531572/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                TGGCGCAGGAACCAGCGATACATCACCGCTCCGATACGCCATTCTCGCAGGTCTGCTTGTGTAATGATCATTCGTTAGTGCCGGTGATAGCCATCGACCATGCTTTTAAAGGTTTCGTGTGGCGTACGCCCCGTGGTGCAAAGATAAAGATGACCGAAGATAAAAAAGAGGCTTATAAATGCCAGAGCAAAATGTGTCTGCAATAACCAGTATCTTACGCCAGGAAACACATCTCCCACGGCTTGCGGATAGAGACACAGCAGC  >  NC_000913/1749489‑1749752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑