Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,781,438 T→C V15A (GTT→GCT)  ydiS → putative oxidoreductase with FAD/NAD(P)‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,781,4380TC100.0% 168.9 / NA 47V15A (GTT→GCT) ydiSputative oxidoreductase with FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/23);  total (24/23)

AGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCAAT  >  NC_000913/1781303‑1781567
                                                                                                                                       |                                                                                                                                 
aGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCgctgct                                                                                                                               <  2:248320/140‑1 (MQ=255)
   cgtcCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGt                                                                                                                            >  1:668650/1‑140 (MQ=255)
    gtcCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTa                                                                                                                           >  2:461814/1‑140 (MQ=255)
     tcCCTGCCGTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCTCATCTCCCGCTGTAACCGCAGCGGGAAGGGAGGATAACGAATGTCGGATGACAAATTTGATGCCATTGTAGTCGGTGCGGGCGATGCTGGTAg                                                                                                                          <  2:179506/140‑1 (MQ=255)
     tcCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAg                                                                                                                          <  1:918539/140‑1 (MQ=255)
     tcCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAg                                                                                                                          >  2:124416/1‑140 (MQ=255)
               gATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCa                                                                                                                <  1:742412/140‑1 (MQ=255)
                aTTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGTCAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGCTGCCGCAc                                                                                                               >  1:581465/1‑140 (MQ=255)
                aTTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCa                                                                                                                >  1:1110/1‑139 (MQ=255)
                aTTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCa                                                                                                                <  2:1110/139‑1 (MQ=255)
                aTTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCAc                                                                                                               >  1:370533/1‑140 (MQ=255)
                     ccAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTc                                                                                                          <  2:507089/140‑1 (MQ=255)
                      cAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCa                                                                                                         <  2:12669/140‑1 (MQ=255)
                         ttGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATgg                                                                                                      >  1:244690/1‑140 (MQ=255)
                               cGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAg                                                                                                <  1:461814/140‑1 (MQ=255)
                                gCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGc                                                                                               <  2:77055/140‑1 (MQ=255)
                                     ttCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGc                                                                                          <  2:139644/140‑1 (MQ=255)
                                      tCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCt                                                                                         >  1:381583/1‑140 (MQ=255)
                                       ccccTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTg                                                                                        <  2:69010/140‑1 (MQ=255)
                                          cTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGAt                                                                                     <  2:381583/140‑1 (MQ=255)
                                          cTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGAt                                                                                     <  1:124416/140‑1 (MQ=255)
                                               aaCCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCt                                                                                <  1:849042/140‑1 (MQ=255)
                                                aCCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTg                                                                               >  1:921667/1‑140 (MQ=255)
                                                aCCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTg                                                                               <  2:668650/140‑1 (MQ=255)
                                                aCCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTg                                                                               >  2:544857/1‑140 (MQ=255)
                                                    caTCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGa                                                                           >  2:620698/1‑140 (MQ=255)
                                                            gCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGCCGGATGACAGATTTGATGCCATTGTGGGCGAGGCTGTCGCTGCTATAAGCGTTTCCGGACTCGGCATAGCACGAGCCGGGCTGGATGTCCTGGTGATAGAAcgc                                                                   >  2:252456/1‑140 (MQ=255)
                                                               gTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAActgtct                                                                >  1:843303/1‑135 (MQ=255)
                                                                tAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACgcggcg                                                               <  2:921667/140‑1 (MQ=255)
                                                                    cGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAg                                                           >  1:612770/1‑140 (MQ=255)
                                                                        gCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGcc                                                       >  2:804723/1‑140 (MQ=255)
                                                                                   gCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTaaaaa                                            >  1:79852/1‑140 (MQ=255)
                                                                                    cATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAAc                                           >  1:623919/1‑140 (MQ=255)
                                                                                     aTAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGcc                                                       >  1:374562/1‑127 (MQ=255)
                                                                                     aTAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGcc                                                       <  2:374562/127‑1 (MQ=255)
                                                                                      tAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACAt                                         >  1:853234/1‑140 (MQ=255)
                                                                                      tAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACAt                                         >  2:28290/1‑140 (MQ=255)
                                                                                         cGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGAc                                      >  2:802285/1‑140 (MQ=255)
                                                                                           cATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACcg                                    >  1:796313/1‑140 (MQ=255)
                                                                                              gTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACcggcg                                 >  1:577771/1‑140 (MQ=255)
                                                                                                 ggATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCggcgggc                              <  1:620698/140‑1 (MQ=255)
                                                                                                        aaaTTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTa                       <  2:623919/140‑1 (MQ=255)
                                                                                                        aaaTTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTa                       <  1:858867/140‑1 (MQ=255)
                                                                                                          aTTTGATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATg                     <  1:28290/140‑1 (MQ=255)
                                                                                                              gATGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCca                 <  2:501614/140‑1 (MQ=255)
                                                                                                               aTGCCATTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCcac                <  2:612770/140‑1 (MQ=255)
                                                                                                                    aTTGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACt           >  1:76204/1‑140 (MQ=255)
                                                                                                                     ttGTGGTCGGTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACtt          <  2:796313/140‑1 (MQ=255)
                                                                                                                            cGGTGCGGGCGCTGCTGGTAGCGCTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCATCTTTATGCCCACACACTTGAAGCaa   <  2:581465/140‑1 (MQ=255)
                                                                                                                             ggTGCGGGCGCTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTCTGCCCACACAATTGAAGCAAt  >  2:829686/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                 
AGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACCACATCTCCCGCTGTAAACGCAGCGGGAAGGGAGCATAACGCATGTCGGATGACAAATTTGATGCCATTGTGGTCGGTGCGGGCGTTGCTGGTAGCGTTGCCGCACTGGTCATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCAAT  >  NC_000913/1781303‑1781567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑