Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,794,760	T→C	I131M (ATA→ATG)	btuC ←	vitamin B12 ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,794,760	0	T	C	100.0%	192.7 / NA	54	I131M (ATA→ATG)	btuC	vitamin B12 ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/21);  total (33/21)

TACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCCCACGCCTGCGCCGTTAGAGACGCCAAGTAGTCCAGGTTCTG  >  NC_000913/1794623‑1794896                                                                                                                                          |                                                                                                                                         tACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCAt                                                                                                                                        >  1:414428/1‑140 (MQ=255)           gACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGtgatg                                                                                                                              >  2:50582/1‑140 (MQ=255)                aaaTCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAg                                                                                                                         <  2:339506/140‑1 (MQ=255)                        tGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAg                                                                                                                 <  2:137702/140‑1 (MQ=255)                            gTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAAt                                                                                                             >  2:374468/1‑140 (MQ=255)                                    gTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAg                                                                                                                 >  2:606457/1‑128 (MQ=255)                                    gTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAg                                                                                                                 <  1:606457/128‑1 (MQ=255)                                    gTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGcacaca                                                                                                     <  2:414428/140‑1 (MQ=255)                                    gTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGcacaca                                                                                                     >  1:745081/1‑140 (MQ=255)                                      aGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGc                                                                                                   >  1:146379/1‑140 (MQ=255)                                       gATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGca                                                                                                         <  2:602582/133‑1 (MQ=255)                                       gATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGca                                                                                                         >  1:602582/1‑133 (MQ=255)                                               aCGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGcc                                                                                          <  2:745081/140‑1 (MQ=255)                                                     ttAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTg                                                                                    >  1:59108/1‑140 (MQ=255)                                                     ttAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTg                                                                                    <  1:374468/140‑1 (MQ=255)                                                           cgcTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGt                                                                              >  1:412862/1‑140 (MQ=255)                                                           cgcTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGt                                                                              >  2:309784/1‑140 (MQ=255)                                                           cgcTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGt                                                                              >  2:719103/1‑140 (MQ=255)                                                           cgcTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGt                                                                              >  2:311836/1‑140 (MQ=255)                                                                  gATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGttgccctt                                                                       <  2:412862/140‑1 (MQ=255)                                                                   aTAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGttgcccttg                                                                      >  2:142903/1‑140 (MQ=255)                                                                    tAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGttgcccttgc                                                                     <  2:450912/140‑1 (MQ=255)                                                                     aaTCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGttgcccttgcc                                                                    <  1:311836/140‑1 (MQ=255)                                                                       tCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCa                                                                  <  1:719103/140‑1 (MQ=255)                                                                        cccTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCaa                                                                 >  2:218736/1‑140 (MQ=255)                                                                          cTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGc                                                               >  1:894953/1‑140 (MQ=255)                                                                          cTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGc                                                               >  1:542798/1‑140 (MQ=255)                                                                            aaTGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGTACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCaa                                                             >  1:932162/1‑140 (MQ=255)                                                                             aTGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGttgcccttgc                                                                     >  2:662193/1‑131 (MQ=255)                                                                             aTGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGttgcccttgc                                                                     <  1:662193/131‑1 (MQ=255)                                                                              tGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGTACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATa                                                           <  2:932162/140‑1 (MQ=255)                                                                                cAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATAcc                                                         >  1:209561/1‑140 (MQ=255)                                                                                   cGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACcgcg                                                      <  2:209561/140‑1 (MQ=255)                                                                                   cGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACcgcg                                                      >  1:8338/1‑140 (MQ=255)                                                                                   cGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACcgcg                                                      >  1:853783/1‑140 (MQ=255)                                                                                   cGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACcgcg                                                      >  2:753422/1‑140 (MQ=255)                                                                                   cGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACcgcg                                                      >  1:267455/1‑140 (MQ=255)                                                                                     ccagccagCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCACACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCgcggc                                                    >  1:695662/1‑140 (MQ=255)                                                                                        gccagcAATAACCGACTGGTCGAAAGATGATGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGAt                                                 >  2:617376/1‑140 (MQ=255)                                                                                        gccagcAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGAt                                                 >  2:218601/1‑140 (MQ=255)                                                                                         ccagcAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATa                                                <  1:142903/140‑1 (MQ=255)                                                                                             cAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCTCTTGCCCAAGCAATACCGCGGCGATAAGcc                                            >  1:151761/1‑140 (MQ=255)                                                                                               aTAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGcccc                                          >  1:668544/1‑140 (MQ=255)                                                                                                  aCCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCCCACg                                       >  2:629411/1‑140 (MQ=255)                                                                                                  aCCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCCCACg                                       <  2:668544/140‑1 (MQ=255)                                                                                                      aCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCCCACGCCTg                                   >  1:391991/1‑140 (MQ=255)                                                                                                               aaGATGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCCCACGCCTGCGCCGTTag                          <  2:894953/140‑1 (MQ=255)                                                                                                                  aTGACGACGGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCCCACGCCTGCGCCGTTAgaga                       >  1:905844/1‑140 (MQ=255)                                                                                                                       gacgGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCCCACGCCTGCGCCGTTAGAGACGCCa                  <  1:50582/140‑1 (MQ=255)                                                                                                                       gacgGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCCCACGCCTGCGCCGTTAGAGACGCCa                  <  2:146379/140‑1 (MQ=255)                                                                                                                         cgGGCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCCCACGCCTGCGCCGTTAGAGACGCCAag                <  2:391991/140‑1 (MQ=255)                                                                                                                            gCGAAACGTAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCCCACGCCTGCGCCGTTAGAGACGCCAagtag             >  1:52828/1‑140 (MQ=255)                                                                                                                                    tAAGAGCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCCCACGCCTGCGCCGTTAGAGACGCCAAGTAGTCCAGGtt     <  2:52828/140‑1 (MQ=255)                                                                                                                                       gagCATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCCCACGCCTGCGCCGTTAGAGACGCCAAGTAGTCCAGGTTCTg  >  1:889657/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         TACATCAGCTGACGCAAATCAACTGAGGTGGAAAAGTAGATAGCCCACGTCATTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTGGGGAGTTGCCCTTGCCCAAGCAATACCGCGGCGATAAGCCCCACGCCTGCGCCGTTAGAGACGCCAAGTAGTCCAGGTTCTG  >  NC_000913/1794623‑1794896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑