Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,843,273	T→C	G301G (GGT→GGC)	gdhA →	glutamate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,843,273	0	T	C	100.0%	180.9 / NA	51	G301G (GGT→GGC)	gdhA	glutamate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/24);  total (27/24)

CGTGTGATCACTGCGTCAGACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGACGCCGCGCATCAGCTTATCGCTAATGGCGTTAAAGCCGTCGCCGAAGG  >  NC_000913/1843139‑1843407                                                                                                                                       |                                                                                                                                       cGTGTGATCACTGCGTCAGACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTtggcctggt                                                                                                                                   <  2:47327/140‑1 (MQ=255)     tgATCACTGCGTCAGACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTAt                                                                                                                               <  1:220482/140‑1 (MQ=255)            tGCGTCAGACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTtggcctggt                                                                                                                                   <  1:636304/129‑1 (MQ=255)            tGCGTCAGACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTtggcctggt                                                                                                                                   >  2:636304/1‑129 (MQ=255)                      tCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGa                                                                                                                          >  1:801073/1‑128 (MQ=255)                      tCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGa                                                                                                                          <  2:801073/128‑1 (MQ=255)                      tCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGcc                                                                                                              <  2:129699/140‑1 (MQ=255)                      tCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGcc                                                                                                              <  1:38879/140‑1 (MQ=255)                      tCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGcc                                                                                                              >  1:473620/1‑140 (MQ=255)                        cAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGt                                                                                                            >  2:222351/1‑140 (MQ=255)                             gCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGtct                                                                                                       >  2:909899/1‑140 (MQ=255)                              cACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGtctc                                                                                                      >  2:49184/1‑140 (MQ=255)                              cACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGtctc                                                                                                      >  2:286168/1‑140 (MQ=255)                              cACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGtctc                                                                                                      >  2:122421/1‑140 (MQ=255)                                 tGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTAc                                                                                                   <  1:652235/140‑1 (MQ=255)                                  gTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGcc                                                                                                              <  2:774219/128‑1 (MQ=255)                                  gTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGcc                                                                                                              >  1:774219/1‑128 (MQ=255)                                           gaAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGatat                                                                                         >  1:520226/1‑140 (MQ=255)                                           gaAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGatat                                                                                         >  1:945592/1‑140 (MQ=255)                                           gaAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGatat                                                                                         >  2:534001/1‑140 (MQ=255)                                           gaAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGat                                                                                           <  1:102864/138‑1 (MQ=255)                                           gaAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGat                                                                                           >  2:102864/1‑138 (MQ=255)                                             aaGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCg                                                                                       >  1:418447/1‑140 (MQ=255)                                             aaGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCg                                                                                       >  1:544923/1‑140 (MQ=255)                                             aaGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCATGGTCTCTACCGGTTGATATCg                                                                                       >  2:481892/1‑140 (MQ=255)                                              aGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGc                                                                                      <  2:678207/140‑1 (MQ=255)                                               gCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGcc                                                                                     <  1:286168/140‑1 (MQ=255)                                                   aTTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGc                                                                                 >  1:645035/1‑140 (MQ=255)                                                    ttCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGcc                                                                                <  1:122421/140‑1 (MQ=255)                                                    ttCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGcc                                                                                <  1:749740/140‑1 (MQ=255)                                                         gAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTgcg                                                                           <  1:948347/140‑1 (MQ=255)                                                                aaaCTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCa                                                                    <  1:222351/140‑1 (MQ=255)                                                                  aCTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGa                                                                  <  1:932850/140‑1 (MQ=255)                                                                  aCTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGa                                                                  <  2:473620/140‑1 (MQ=255)                                                                     ggCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATg                                                               <  1:534001/140‑1 (MQ=255)                                                                        aCGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAAc                                                            <  2:234611/140‑1 (MQ=255)                                                                            cTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGa                                                        >  1:943195/1‑140 (MQ=255)                                                                              tATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATg                                                      >  1:539203/1‑140 (MQ=255)                                                                                     aTCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGAcgc                                               >  2:178644/1‑140 (MQ=255)                                                                                      tCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGAcgcc                                              >  1:333066/1‑140 (MQ=255)                                                                                         aaGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGACGCcgcg                                           >  1:39739/1‑140 (MQ=255)                                                                                           gccagccGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGACGCCGCGCa                                         <  2:343014/140‑1 (MQ=255)                                                                                            ccagccGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGACGCCGCGCAt                                        <  1:178644/140‑1 (MQ=255)                                                                                                           gAGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGACGCCGCGCATCAGCTTATCGCTAAt                         <  1:49184/140‑1 (MQ=255)                                                                                                            aGTGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGACGCCGCGCATCAGCTTATCGCTAATg                        <  1:909899/140‑1 (MQ=255)                                                                                                              tGGCAGATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGACGCCGCGCATCAGCTTATCGCTAATGGc                      >  2:236719/1‑140 (MQ=255)                                                                                                                   gATTACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGACGCCGCGCATCAGCTTATCGCTAATGGCGTTaa                 >  1:876151/1‑140 (MQ=255)                                                                                                                     ttACGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGACGCCGCGCATCAGCTTATCGCTAATGGCGTTAAAg               <  2:945592/140‑1 (MQ=255)                                                                                                                       aCGCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCGACCCAGAATGAACTGGATGTTGACGCCGCGCATCAGCTTATCGCTAATGGCGTTAAAGcc             >  2:860340/1‑140 (MQ=255)                                                                                                                         gCCAAAGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGACGCCGCGCATCAGCTTATCGCTAATGGCGTTAAAGCCGt           <  2:418447/140‑1 (MQ=255)                                                                                                                             aaGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGACGCCGCGCATCAGCTTATCGCTAATGGCGTTAAAGCCGTCGcc       >  2:138242/1‑140 (MQ=255)                                                                                                                              aGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGACGCCGCGCATCAGCTTATCGCTAATGGCGTTAAAGCCGTCGCCg      >  1:582762/1‑140 (MQ=255)                                                                                                                              aGAATTTGGCCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGACGCCGCGCATCAGCTTATCGCTAATGGCGTTAAAGCCGTCGCCg      >  1:294756/1‑140 (MQ=255)                                                                                                                                  tttGGCCTGGTCTGTCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGACGCCGCGCATCAGCTTATCGCTAATGGCGTTAAAGCCGTCGCCGAAgg  <  2:645035/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       CGTGTGATCACTGCGTCAGACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGACGCCGCGCATCAGCTTATCGCTAATGGCGTTAAAGCCGTCGCCGAAGG  >  NC_000913/1843139‑1843407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑