Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,844,210	T→C	D221G (GAC→GGC)	ynjI ←	DUF1266 domain‑containing protein YnjI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,844,210	0	T	C	100.0%	175.2 / NA	49	D221G (GAC→GGC)	ynjI	DUF1266 domain‑containing protein YnjI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/21);  total (28/21)

CAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCTAT  >  NC_000913/1844075‑1844345                                                                                                                                          |                                                                                                                                        caggccagTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGa                                                                                                                                     >  1:535135/1‑140 (MQ=255) caggccagTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGa                                                                                                                                     >  1:391823/1‑140 (MQ=255)   ggccagTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAgg                                                                                                                                   >  1:630924/1‑140 (MQ=255)   ggccagTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAgg                                                                                                                                   >  2:786745/1‑140 (MQ=255)                   aTTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAAttt                                                                                                                   >  2:272777/1‑140 (MQ=255)                   aTTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAAt                                                                                                                     <  1:196959/138‑1 (MQ=255)                   aTTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAAt                                                                                                                     >  2:196959/1‑138 (MQ=255)                     tGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTAt                                                                                                                 <  1:302466/140‑1 (MQ=255)                      gTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTAtt                                                                                                                >  1:365783/1‑140 (MQ=255)                       tCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTc                                                                                                               >  1:656324/1‑140 (MQ=255)                           gTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTcaaca                                                                                                           <  2:630924/140‑1 (MQ=255)                                     gCTGTGCTTGATATCGAGATCATCCATGATAATTCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAAc                                                                                                 <  1:70479/140‑1 (MQ=255)                                     gCTGTGCTTGATATCGAGATCATCCATGATAATTCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAAc                                                                                                 <  2:391823/140‑1 (MQ=255)                                      cTGTGCTTGATATCGAGATCATCCATGATAATTCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAAcc                                                                                                <  1:786745/140‑1 (MQ=255)                                         tgCTTGATATCGAGATCATCCATGATAATTCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGc                                                                                             >  1:435177/1‑140 (MQ=255)                                         tgCTTGATATCGAGATCATCCATGATAATTCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGc                                                                                             >  1:527334/1‑140 (MQ=255)                                          gCTTGATATCGAGATCATCCATGATAATTCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTg                                                                                              <  1:400814/140‑1 (MQ=255)                                                  tCGAGATCATCCATGATAATTCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCt                                                                                    <  2:128886/140‑1 (MQ=255)                                                   cGAGATCATCCATGATAATTCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCtt                                                                                   >  1:934569/1‑140 (MQ=255)                                                     agaTCATCCATGATAATTCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTtcc                                                                                 >  2:400910/1‑140 (MQ=255)                                                           tcCATGATAATTCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATg                                                                           >  2:579957/1‑140 (MQ=255)                                                            ccATGATAATTCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGt                                                                          <  1:260016/140‑1 (MQ=255)                                                             cATGATAATTCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGtt                                                                         <  2:435177/140‑1 (MQ=255)                                                             cATGATAATTCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGtt                                                                         <  2:656324/140‑1 (MQ=255)                                                                     ttCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTaa                                                                 >  2:305456/1‑140 (MQ=255)                                                                       ccgccgCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAg                                                               >  2:511284/1‑140 (MQ=255)                                                                         gccgccCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGt                                                             >  2:401478/1‑140 (MQ=255)                                                                          ccgccCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTa                                                            >  2:760319/1‑140 (MQ=255)                                                                          ccgccCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTa                                                            >  1:871388/1‑140 (MQ=255)                                                                          ccgccCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTa                                                            >  2:458016/1‑140 (MQ=255)                                                                                       cTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGt                                               >  1:365632/1‑140 (MQ=255)                                                                                          cGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGc                                            >  2:193772/1‑140 (MQ=255)                                                                                           gCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGcc                                           <  1:579957/140‑1 (MQ=255)                                                                                           gCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGcc                                           >  1:119124/1‑140 (MQ=255)                                                                                                       ccGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATg                               <  2:670286/140‑1 (MQ=255)                                                                                                        cGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGc                              <  1:152275/140‑1 (MQ=255)                                                                                                        cGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGc                              <  1:272777/140‑1 (MQ=255)                                                                                                           aGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTcc                           >  2:194985/1‑140 (MQ=255)                                                                                                           aGCTATGATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTcc                           >  1:579606/1‑140 (MQ=255)                                                                                                                 gATTAGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAg                     <  1:305456/140‑1 (MQ=255)                                                                                                                     aGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTaa                      >  1:655519/1‑135 (MQ=255)                                                                                                                     aGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTaa                      <  2:655519/135‑1 (MQ=255)                                                                                                                     aGCGCA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGcac                 >  1:798070/1‑140 (MQ=255)                                                                                                                       cgcA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACaa               >  1:329537/1‑140 (MQ=255)                                                                                                                        gcA‑‑ATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAAt              <  2:798070/140‑1 (MQ=255)                                                                                                                                  agaTATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCg      <  1:760319/140‑1 (MQ=255)                                                                                                                                   gaTATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGc     >  1:33931/1‑140 (MQ=255)                                                                                                                                    atatAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCt    <  2:365632/140‑1 (MQ=255)                                                                                                                                      ataGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCTAt  <  1:400910/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        CAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCA‑‑ATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCTAT  >  NC_000913/1844075‑1844345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑