Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,081,543	A→G	G335G (GGT→GGC)	dacD ←	D‑alanyl‑D‑alanine carboxypeptidase DacD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,081,543	0	A	G	100.0%	153.5 / NA	44	G335G (GGT→GGC)	dacD	D‑alanyl‑D‑alanine carboxypeptidase DacD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/25);  total (19/25)

CAGGCGAGAAAACATGCTGCCTTCCCCGACAGATTCCAGGGTAACCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCA  >  NC_000913/2081408‑2081678                                                                                                                                        |                                                                                                                                        cAGGCGAGAAAACATGCTGCCTTCCCCGACAGATTCCAGGGTAACCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCAt                                                                                                                                     <  1:214620/140‑1 (MQ=255) cAGGCGAGAAAACATGCTGCCTTCCCCGACAGATTCCAGGGTAACCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCAt                                                                                                                                     <  2:829337/140‑1 (MQ=255)  aGGCGAGAAAACATGCTGCCTTCCCCGACAGATTCCAGGGTAACCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTANTTGGCGCGGTGAGTTCTTTGCCATc                                                                                                                                    <  2:473568/140‑1 (MQ=255)  aGGCGAGAAAACATGCTGCCTTCCCCGACAGATTCCAGGGTAACCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATc                                                                                                                                    >  2:434116/1‑140 (MQ=255)  aGGCGAGAAAACATGCTGCCTTCCCCGACAGATTCCAGGGTAACCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATc                                                                                                                                    <  1:832557/140‑1 (MQ=255)    gCGAGAAAACATGCTGCCTTCCCCGACAGATTCCAGGGTAACCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCaa                                                                                                                                  >  1:856055/1‑140 (MQ=255)     cGAGAAAACATGCTGCCTTCCCCGACAGATTCCAGGGTAACCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAg                                                                                                                                 <  1:323734/140‑1 (MQ=255)       agaAAACATGCTGCCTTCCCCGACAGATTCCAGGGTAACCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAggg                                                                                                                               >  1:242358/1‑140 (MQ=255)             cATGCTGCCTTCCCCGACAGATTCCAGGGTAACCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATAtt                                                                                                                         <  2:874549/140‑1 (MQ=255)              aTGCTGCCTTCCCCGACAGATTCCAGGGTAACCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATAttt                                                                                                                        >  1:308197/1‑140 (MQ=255)                                 aTTCCAGGGTAACCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATA‑TTGGCTTTGATATGTGGAAttt                                                                                                    >  1:933264/1‑140 (MQ=255)                                    ccAGGGTAACCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATA‑TTGGCTTTGATATGTGGAATTTCgg                                                                                                 <  2:933264/140‑1 (MQ=255)                                       gggTAACCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCt                                                                                               <  2:955857/140‑1 (MQ=255)                                       gggTAACCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCt                                                                                               <  2:838795/140‑1 (MQ=255)                                       gggTAACCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCt                                                                                               <  1:335620/140‑1 (MQ=255)                                            aCCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCt                                                                                               >  2:176921/1‑135 (MQ=255)                                            aCCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCt                                                                                               <  1:176921/135‑1 (MQ=255)                                            aCCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCgg                                                                                          >  1:241908/1‑140 (MQ=255)                                                      aGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATc                                                                                >  1:791515/1‑140 (MQ=255)                                                         gcgcCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAg                                                                             <  2:856055/140‑1 (MQ=255)                                                         gcgcCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAg                                                                             <  2:465874/140‑1 (MQ=255)                                                                    ttATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGCTATGTGGAATTTCGGATGTCGGTAGCCCCATCCAGAACTCTTgttc                                                                  <  2:281384/140‑1 (MQ=255)                                                                          cGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTgttccgttcc                                                            >  2:601809/1‑140 (MQ=255)                                                                            gTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCa                                                          >  1:702425/1‑140 (MQ=255)                                                                             tCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAg                                                         >  1:413956/1‑140 (MQ=255)                                                                             tCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAg                                                         >  2:25699/1‑140 (MQ=255)                                                                              ggTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAgg                                                        <  2:702425/139‑1 (MQ=255)                                                                              cGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGCATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAgg                                                        <  1:434116/140‑1 (MQ=255)                                                                                tAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTc                                                      >  2:59370/1‑140 (MQ=255)                                                                                       cAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGt                                                       <  1:141187/132‑1 (MQ=255)                                                                                       cAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGt                                                       >  2:141187/1‑132 (MQ=255)                                                                                        aaTTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATAtttt                                              <  2:791515/140‑1 (MQ=255)                                                                                            tCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCttt                                          <  1:25699/140‑1 (MQ=255)                                                                                              cccTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTAt                                        <  2:413956/140‑1 (MQ=255)                                                                                                 tACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGc                                     <  2:242358/140‑1 (MQ=255)                                                                                                 tACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGc                                     <  1:568347/140‑1 (MQ=255)                                                                                                   cccGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATAttt                                               >  1:911037/1‑128 (MQ=255)                                                                                                   cccGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATAttt                                               <  2:911037/128‑1 (MQ=255)                                                                                                      gCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATAc                                >  2:446397/1‑140 (MQ=255)                                                                                                        tGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCttt                                          <  2:100896/128‑1 (MQ=255)                                                                                                        tGATGGGCGCTAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCttt                                          >  1:100896/1‑128 (MQ=255)                                                                                                                  tAATTGGCGCGGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGcgttcc                    >  1:453375/1‑140 (MQ=255)                                                                                                                          gcgGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGAc            <  2:453375/140‑1 (MQ=255)                                                                                                                           cgGTGAGTTCTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGAcc           >  1:845045/1‑140 (MQ=255)                                                                                                                                    cTTTGCCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCa  <  1:446397/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        CAGGCGAGAAAACATGCTGCCTTCCCCGACAGATTCCAGGGTAACCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCA  >  NC_000913/2081408‑2081678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑