Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,147,047	T→C	D165G (GAC→GGC)	alkA ←	DNA‑3‑methyladenine glycosylase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,147,047	0	T	C	100.0%	171.1 / NA	48	D165G (GAC→GGC)	alkA	DNA‑3‑methyladenine glycosylase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/21);  total (27/21)

TTGCAGCGTTTTCATCGCCTGCTCCACATCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTGGCGGTCAATTTTGCCGCCATCGCCACGCTCACCAGTTGGCCTAAAATCG  >  NC_000913/2146911‑2147182                                                                                                                                         |                                                                                                                                        ttGCAGCGTTTTCATCGCCTGCTCCACATCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCctg                                                                                                                                      >  2:909381/1‑140 (MQ=255)         ttttCATCGCCTGCTCCACATCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATTCCTGCGGGCCTGCTGCTGcc                                                                                                                              <  1:219032/140‑1 (MQ=255)                   cTGCTCCACATCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAg                                                                                                                    >  2:883627/1‑140 (MQ=255)                      cTCCACATCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCg                                                                                                                 >  1:534460/1‑140 (MQ=255)                            aTCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGa                                                                                                           <  1:527985/140‑1 (MQ=255)                            aTCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGa                                                                                                           >  2:261739/1‑140 (MQ=255)                                  cGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGa                                                                                                     <  2:534460/140‑1 (MQ=255)                                     tATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCgg                                                                                                             <  2:828528/129‑1 (MQ=255)                                     tATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCgg                                                                                                             >  1:828528/1‑129 (MQ=255)                                               ggTAAGGTGTCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGaaa                                                                                        >  1:364741/1‑140 (MQ=255)                                               ggTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGaaa                                                                                        >  1:196589/1‑140 (MQ=255)                                               ggTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGaaa                                                                                        >  2:730511/1‑140 (MQ=255)                                               ggTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGaaa                                                                                        >  1:620152/1‑140 (MQ=255)                                                    ggTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAatcat                                                                                   >  1:847065/1‑140 (MQ=255)                                                      tGCCCTCCAGCGCCGCATTTGCCAGATGAATCCGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTACTGCCTGCGGGCCTGCTGCTGCCAGCCGTTGAGGCGTCGGGAAGTAGATATACTCCGGGAAATCATcc                                                                                 >  2:840623/1‑140 (MQ=255)                                                      tGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATcc                                                                                 >  2:451387/1‑140 (MQ=255)                                                          cTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGcc                                                                             >  2:582027/1‑140 (MQ=255)                                                             cAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGtt                                                                          <  2:386540/140‑1 (MQ=255)                                                             cAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGtt                                                                          <  2:364741/140‑1 (MQ=255)                                                             cAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGtt                                                                          <  1:404708/140‑1 (MQ=255)                                                             cAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGtt                                                                          <  1:883627/140‑1 (MQ=255)                                                              aGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTc                                                                         <  1:582027/140‑1 (MQ=255)                                                                  ccgcATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCa                                                                     >  1:262339/1‑140 (MQ=255)                                                                   cgcATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCAt                                                                    <  1:730511/140‑1 (MQ=255)                                                                     cATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATaa                                                                  >  1:658591/1‑140 (MQ=255)                                                                     cATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATaa                                                                  <  2:620152/140‑1 (MQ=255)                                                                          gCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCtg                                                             <  2:658591/140‑1 (MQ=255)                                                                           ccAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCtgt                                                            <  1:909381/140‑1 (MQ=255)                                                                                    tCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTg                                                   >  1:598146/1‑140 (MQ=255)                                                                                     cAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTgg                                                  <  2:262339/140‑1 (MQ=255)                                                                                     cAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTgg                                                  >  2:33634/1‑140 (MQ=255)                                                                                             ctGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTGGCGGTCAAt                                          >  1:931680/1‑140 (MQ=255)                                                                                                    tttCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTGGCGGTCAATTTTgccg                                   >  2:17497/1‑140 (MQ=255)                                                                                                     ttCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTGGCg                                                >  1:394756/1‑126 (MQ=255)                                                                                                     ttCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTGGCg                                                <  2:394756/126‑1 (MQ=255)                                                                                                     ttCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTGGCGGTCAATTTTgccgc                                  <  1:17497/140‑1 (MQ=255)                                                                                                     ttCAACGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTGGCGGTCAATTTTgccgc                                  >  1:770730/1‑140 (MQ=255)                                                                                                        aaCGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTGGCGGTCAATTTTGCCGCCAt                               >  2:613332/1‑140 (MQ=255)                                                                                                          cGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTGGCGGTCAATTTTGCCGCCATCg                             <  2:598146/140‑1 (MQ=255)                                                                                                          cGGCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTGGCGGTCAATTTTGCCGCCATCg                             <  2:213953/140‑1 (MQ=255)                                                                                                            gCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTGGCGGTCAATTTTGCCGCCATCGcc                           <  1:460770/140‑1 (MQ=255)                                                                                                            gCATACCTAACGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTGGCGGTCAATTTTGCCGCCATCGcc                           <  2:847065/140‑1 (MQ=255)                                                                                                                     aCGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCTGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTGGCGGTCAATTTTgccgc                                  <  2:666540/124‑1 (MQ=255)                                                                                                                     aCGCTTTTAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTGGCGGTCAATTTTgccgc                                  >  1:666540/1‑124 (MQ=255)                                                                                                                         ttttAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTGGCGGTCAATTTTGCCGCCATCGCCACGCTCACCAGtt              >  2:739890/1‑140 (MQ=255)                                                                                                                           ttAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTGGCGGTCAATTTTGCCGCCATCGCCACGCTCACCAGTTgg            >  1:619855/1‑140 (MQ=255)                                                                                                                            tAATGCCTGCGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTGGCGGTCAATTTTGCCGCCATCGCCACGCTCACCAGTTGGc           >  1:612710/1‑140 (MQ=255)                                                                                                                                     cGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTGGCGGTCAATTTTGCCGCCATCGCCACGCTCACCAGTTGGCCTAAAATCg  >  2:680126/1‑140 (MQ=255)                                                                                                                                     cGGGCCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTGGCGGTCAATTTTGCCGCCATCGCCACGCTCACCAGTTGGCCTAAAATCg  >  1:632146/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TTGCAGCGTTTTCATCGCCTGCTCCACATCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTGGCGGTCAATTTTGCCGCCATCGCCACGCTCACCAGTTGGCCTAAAATCG  >  NC_000913/2146911‑2147182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑