Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	34,120	(T)9→8	intergenic (+82/‑180)	carB → / → caiF	carbamoyl phosphate synthetase subunit beta/DNA‑binding transcriptional activator CaiF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	34,112	0	T	.	100.0%	363.1 / NA	87	intergenic (+74/‑188)	carB/caiF	carbamoyl phosphate synthetase subunit beta/DNA‑binding transcriptional activator CaiF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (45/42);  total (45/42)

CGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGCCTTGTTTAAT  >  NC_000913/33974‑34247                                                                                                                                           |                                                                                                                                        cgcTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGtt                                                                                                                                        >  2:415543/1‑140 (MQ=255)   cTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGtttt                                                                                                                                      >  1:1444503/1‑140 (MQ=255)            gATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCAGAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCa                                                                                                                            <  1:1057248/140‑1 (MQ=255)            gATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCTTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCa                                                                                                                            <  2:1341379/140‑1 (MQ=255)            gATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCa                                                                                                                            <  2:608562/140‑1 (MQ=255)             aTGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCAt                                                                                                                           >  1:371781/1‑140 (MQ=255)                cGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTaa                                                                                                                        >  1:2829/1‑140 (MQ=255)                                ttCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGa                                                                                                        >  2:12086/1‑140 (MQ=255)                                ttCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGa                                                                                                        <  2:2829/140‑1 (MQ=255)                                       cAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGata                                                                                                 >  1:694052/1‑140 (MQ=255)                                               gcacgcacAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTtgat                                                                                         >  1:1293512/1‑140 (MQ=255)                                               gcacgcacAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTtgat                                                                                         >  1:1108081/1‑140 (MQ=255)                                                  cgcacAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGAc                                                                                      <  2:626294/140‑1 (MQ=255)                                                    cacaGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACAt                                                                                    >  2:212793/1‑140 (MQ=255)                                                    cacaGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACAt                                                                                    >  2:1149/1‑140 (MQ=255)                                                     acaGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATa                                                                                   <  2:104605/140‑1 (MQ=255)                                                       aGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAg                                                                                 <  2:1108081/140‑1 (MQ=255)                                                       aGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAg                                                                                 <  2:1293512/140‑1 (MQ=255)                                                        gATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGc                                                                                >  1:1013584/1‑140 (MQ=255)                                                        gATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGc                                                                                >  1:347529/1‑140 (MQ=255)                                                         aTCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCa                                                                               >  1:294870/1‑140 (MQ=255)                                                             aataatAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGAt                                                                           <  1:16373/140‑1 (MQ=255)                                                             aataatAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGAt                                                                           <  1:1040117/140‑1 (MQ=255)                                                              ataataGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGAtt                                                                          <  2:347529/140‑1 (MQ=255)                                                                   aGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGAtt                                                                          <  2:673307/135‑1 (MQ=255)                                                                   aGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGAtt                                                                          >  1:673307/1‑135 (MQ=255)                                                                   aGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCt                                                                     >  2:461109/1‑140 (MQ=255)                                                                   aGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCt                                                                     >  2:474421/1‑140 (MQ=255)                                                                   aGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCt                                                                     >  2:1302852/1‑140 (MQ=255)                                                                   aGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCt                                                                     >  1:116934/1‑140 (MQ=255)                                                                     cGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTca                                                                   <  2:1385970/140‑1 (MQ=255)                                                                     cGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTca                                                                   >  1:770584/1‑140 (MQ=255)                                                                     cGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTca                                                                   >  1:1021392/1‑140 (MQ=255)                                                                     cGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTca                                                                   >  1:1053220/1‑140 (MQ=255)                                                                     cGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTca                                                                   <  1:415543/140‑1 (MQ=255)                                                                     cGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTca                                                                   >  1:1379773/1‑140 (MQ=255)                                                                       tgtCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTcaca                                                                 >  2:425468/1‑140 (MQ=255)                                                                       tgtCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTcaca                                                                 >  1:1478993/1‑140 (MQ=255)                                                                         tCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTc                                                                    <  2:1525012/135‑1 (MQ=255)                                                                         tCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTc                                                                    >  1:1525012/1‑135 (MQ=255)                                                                         tCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACt                                                               >  1:112766/1‑140 (MQ=255)                                                                          cATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACtt                                                              >  2:79475/1‑140 (MQ=255)                                                                            tGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTca                                                                   <  2:1528949/133‑1 (MQ=255)                                                                            tGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTca                                                                   >  1:1528949/1‑133 (MQ=255)                                                                            tGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTAt                                                            <  1:425468/140‑1 (MQ=255)                                                                             ggCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATc                                                           <  1:79475/140‑1 (MQ=255)                                                                              gCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCg                                                          >  1:51671/1‑140 (MQ=255)                                                                               cAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGa                                                         <  2:51671/140‑1 (MQ=255)                                                                                aGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGAc                                                        <  2:1053220/140‑1 (MQ=255)                                                                                  atatTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACgg                                                      >  2:902237/1‑140 (MQ=255)                                                                                  atatTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACgg                                                      >  1:1457068/1‑140 (MQ=255)                                                                                  atatTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACgg                                                      >  1:85873/1‑140 (MQ=255)                                                                                  atatTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACgg                                                      >  1:432625/1‑140 (MQ=255)                                                                                  atatTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACgg                                                      <  2:1494729/140‑1 (MQ=255)                                                                                    atTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTg                                                    <  2:116934/140‑1 (MQ=255)                                                                                    atTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTg                                                    >  1:908813/1‑140 (MQ=255)                                                                                    atTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTg                                                    >  2:393167/1‑140 (MQ=255)                                                                                    atTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTg                                                    <  2:303688/140‑1 (MQ=255)                                                                                    atTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTg                                                    <  2:294870/140‑1 (MQ=255)                                                                                       tttCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAg                                                 <  2:998435/140‑1 (MQ=255)                                                                                       tttCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAg                                                 >  2:574888/1‑140 (MQ=255)                                                                                       tttCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAg                                                 <  1:393167/140‑1 (MQ=255)                                                                                       tttCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAg                                                 <  2:1457068/140‑1 (MQ=255)                                                                                        ttCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGt                                                >  2:97745/1‑140 (MQ=255)                                                                                            tCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCa                                            <  1:212793/140‑1 (MQ=255)                                                                                            tCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCa                                            <  2:1188217/140‑1 (MQ=255)                                                                                            tCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCa                                            <  2:908813/140‑1 (MQ=255)                                                                                            tCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCa                                            <  1:902237/140‑1 (MQ=255)                                                                                            tCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCa                                            <  1:844237/140‑1 (MQ=255)                                                                                            tCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCa                                            <  1:491176/140‑1 (MQ=255)                                                                                              cGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATa                                          <  2:1478993/140‑1 (MQ=255)                                                                                                cTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGc                                             <  1:714373/135‑1 (MQ=255)                                                                                                cTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGc                                             >  2:714373/1‑135 (MQ=255)                                                                                                   aTTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATc                                     <  1:461109/140‑1 (MQ=255)                                                                                                   aTTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATc                                     <  1:1149/140‑1 (MQ=255)                                                                                                      tGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGat                                  <  1:1302852/140‑1 (MQ=255)                                                                                                       gATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGata                                 >  2:126222/1‑140 (MQ=255)                                                                                                             aTAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCca                           <  2:1379773/140‑1 (MQ=255)                                                                                                                        ggTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAata                <  2:432625/140‑1 (MQ=255)                                                                                                                           tctcTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATgg             >  2:1530391/1‑140 (MQ=255)                                                                                                                            ctctGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGc            <  1:126222/140‑1 (MQ=255)                                                                                                                             tctGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGcc           <  2:879540/140‑1 (MQ=255)                                                                                                                              ctGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGCCt          >  1:280198/1‑140 (MQ=255)                                                                                                                               tgatgaGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGCCtt         >  2:825433/1‑140 (MQ=255)                                                                                                                                gatgaGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGCCTTg        >  1:574976/1‑140 (MQ=255)                                                                                                                                  tgaGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGCCTTGtt      >  1:74020/1‑140 (MQ=255)                                                                                                                                    aGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGCCTTGTTTa    >  2:268285/1‑140 (MQ=255)                                                                                                                                      gACCG‑TTTTTTTTGCCCATTGAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGCCTTGTTTAAt  >  2:741392/1‑140 (MQ=255)                                                                                                                                      gACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGCCTTGTTTAAt  >  2:150744/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                        CGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGCCTTGTTTAAT  >  NC_000913/33974‑34247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑