Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,650,599	(A)5→4	coding (363/426 nt)	arsC →	arsenate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,650,595	0	A	.	98.6%	294.8 / ‑2.4	73	coding (359/426 nt)	arsC	arsenate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base . (37/35);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATAAATTTACTGACGATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGGTTGTTAG‑AAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCG  >  NC_000913/3650457‑3650730                                                                                                                                           |                                                                                                                                         aTAAATTTACTGACGATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCaa                                                                                                                                         <  2:476184/140‑1 (MQ=255)       ttACTGACGATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCaaaaggcg                                                                                                                                   <  2:743082/140‑5 (MQ=255)        tACTGACGATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGgcgc                                                                                                                                 >  2:1271057/1‑140 (MQ=255)         aCTGACGATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCa                                                                                                                                <  1:623642/140‑1 (MQ=255)         aCTGACGATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCa                                                                                                                                <  1:325456/140‑1 (MQ=255)         aCTGACGATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCa                                                                                                                                <  1:279584/140‑1 (MQ=255)         aCTGACGATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCa                                                                                                                                <  1:367678/140‑1 (MQ=255)         aCTGACGATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCa                                                                                                                                <  2:8850/140‑1 (MQ=255)         aCTGACGATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCa                                                                                                                                <  1:1447558/140‑1 (MQ=255)         aCTGACGATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCa                                                                                                                                <  1:1271057/140‑1 (MQ=255)         aCTGACGATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCa                                                                                                                                <  1:1125207/140‑1 (MQ=255)         aCTGACGATCGATTAATAGAGTTTACGCTTGAGCAGCCGATTCTGATTAATCGCGTGATTGTGGTGACGTGGTTGGGAACTCGGCTGTGCCGCCCTTCAGAAGTGGTGCTCGAAATTCTGCCAGATGCG‑AAAAAGGCGCa                                                                                                                                <  2:939453/140‑1 (MQ=255)          cTGACGATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCAt                                                                                                                               >  2:445901/1‑140 (MQ=255)          cTGACGATCGGTTAATCGACTTTATGCTTCAGCACCCAATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCAt                                                                                                                               >  1:196593/1‑140 (MQ=255)           tGACGATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCAtt                                                                                                                              <  1:1273669/140‑1 (MQ=255)               gATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTcc                                                                                                                          >  2:903540/1‑140 (MQ=255)               gATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTcc                                                                                                                          >  2:115713/1‑140 (MQ=255)                aTCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCa                                                                                                                         >  2:597670/1‑140 (MQ=255)                aTCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCa                                                                                                                         >  2:589468/1‑140 (MQ=255)                aTCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCa                                                                                                                         >  1:1298273/1‑140 (MQ=255)                   ggTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAgg                                                                                                                      <  2:409133/140‑1 (MQ=255)                     ttAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGaa                                                                                                                    >  1:385815/1‑140 (MQ=255)                       aaTCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGa                                                                                                                  <  1:907696/140‑1 (MQ=255)                         tCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATg                                                                                                                >  1:1314454/1‑140 (MQ=255)                           gACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGc                                                                                                              >  1:911705/1‑140 (MQ=255)                             cTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCga                                                                                                            >  2:33800/1‑140 (MQ=255)                              tttATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCgag                                                                                                           >  2:456722/1‑140 (MQ=255)                               ttATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCgaga                                                                                                          >  2:328453/1‑140 (MQ=255)                               ttATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCgaga                                                                                                          >  2:1022879/1‑140 (MQ=255)                                 aTGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGaaa                                                                                                        >  2:864529/1‑140 (MQ=255)                                 aTGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGaaa                                                                                                        <  1:328453/140‑1 (MQ=255)                                     ttCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGaa                                                                                                                    <  1:718924/124‑1 (MQ=255)                                     ttCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGaa                                                                                                                    >  2:718924/1‑124 (MQ=255)                                      tCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGt                                                                                                   <  2:187357/140‑1 (MQ=255)                                       cAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGtt                                                                                                  >  1:706492/1‑140 (MQ=255)                                         gCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTtga                                                                                                <  1:153842/140‑1 (MQ=255)                                          cACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTtgat                                                                                               >  1:644571/1‑140 (MQ=255)                                             ccGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGaa                                                                                            <  2:911705/140‑1 (MQ=255)                                               gATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGc                                                                                          >  2:405249/1‑140 (MQ=255)                                               gATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGc                                                                                          >  1:1189957/1‑140 (MQ=255)                                                     gATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTaa                                                                                    <  2:505021/140‑1 (MQ=255)                                                      aTTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAg                                                                                   <  1:903540/140‑1 (MQ=255)                                                      aTTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAg                                                                                   <  1:115713/140‑1 (MQ=255)                                                          aTCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGcc                                                                               >  1:923411/1‑140 (MQ=255)                                                           tCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCt                                                                              >  1:1046434/1‑140 (MQ=255)                                                           tCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCt                                                                              >  2:1467090/1‑140 (MQ=255)                                                              cccGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGaa                                                                           >  1:758280/1‑140 (MQ=255)                                                                      tggtgACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAgcg                                                                   >  2:987016/1‑140 (MQ=255)                                                                       ggtgACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAgcgg                                                                  <  1:589468/140‑1 (MQ=255)                                                                         tgACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAgcggcg                                                                <  1:405249/140‑1 (MQ=255)                                                                         tgACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAgcggcg                                                                <  2:385815/140‑1 (MQ=255)                                                                           aCGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGat                                                              <  1:33800/140‑1 (MQ=255)                                                                            cgccgcTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATa                                                                        <  2:1479152/129‑1 (MQ=255)                                                                            cgccgcTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATa                                                                        >  1:1479152/1‑129 (MQ=255)                                                                             gccgcTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGatat                                                            >  2:1405539/1‑140 (MQ=255)                                                                             gccgcTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGat                                                              <  1:629092/138‑1 (MQ=255)                                                                             gccgcTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGat                                                              >  2:629092/1‑138 (MQ=255)                                                                              ccgcTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATc                                                           <  2:276384/140‑1 (MQ=255)                                                                                gcTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATccc                                                         <  2:1298273/140‑1 (MQ=255)                                                                                     gAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCca                                                    <  1:1351488/140‑1 (MQ=255)                                                                                         tCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAgg                                                >  1:716008/1‑140 (MQ=255)                                                                                          cGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGgt                                               <  1:1022879/140‑1 (MQ=255)                                                                                          cGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGgt                                               <  2:1227968/140‑1 (MQ=255)                                                                                          cGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGgt                                               <  2:706492/140‑1 (MQ=255)                                                                                              tgtgCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTATCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGgttgtt                                           >  2:294734/1‑140 (MQ=255)                                                                                                tgCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGGTTGTTAg                                         >  1:757824/1‑140 (MQ=255)                                                                                                tgCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGGTTGTTAg                                         >  2:401278/1‑140 (MQ=255)                                                                                                     cccTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGGTTGTTAG‑AAAAg                                   >  2:848031/1‑140 (MQ=255)                                                                                                      ccTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGGTTGTTAG‑AAAAGt                                  <  2:644571/140‑1 (MQ=255)                                                                                                          cAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGGTTGTTAG‑AAAAGTgcgc                              <  2:1046434/140‑1 (MQ=255)                                                                                                           aGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGGTTGTTAG‑AAAAGTGCGCt                             <  1:445901/140‑1 (MQ=255)                                                                                                                  gtgCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGGTTGTTAGAAAAAGTGCGCtttattt                       >  1:1507157/1‑140 (MQ=255)                                                                                                                     cTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGGTTGTTAG‑AAAAGTGCGCTTTATTTATGt                   >  2:1366564/1‑140 (MQ=255)                                                                                                                                    gATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAG‑AAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGgc   <  1:118244/140‑1 (MQ=255)                                                                                                                                      tGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGGTTGTTAG‑AAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGgcg  >  2:1213957/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                         ATAAATTTACTGACGATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGGTTGTTAG‑AAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCG  >  NC_000913/3650457‑3650730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑