BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,589,698	(T)_7→6	coding (1582/2151 nt)	btsT ←	pyruvate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,589,692	0	T	.	99.3%	560.8 / ‑2.2	136	coding (1588/2151 nt)	btsT	pyruvate:H(+) symporter
Reads supporting (aligned to +/- strand): ref base T (0/1); new base . (72/63); total (72/64)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.45e-01

Only 100 of 139 total aligned reads displayed.

TCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGC  >  NC_000913/4589557‑4589820

tCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGGttttt                                                                                                                              >  1:269881/1‑140 (MQ=255)

       ggAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGa                                                                                                                       <  1:850651/140‑1 (MQ=255)

         agaTACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACg                                                                                                                    >  2:820178/1‑140 (MQ=255)

            tACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGa                                                                                                                 >  1:1039427/1‑140 (MQ=255)

            tACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGa                                                                                                                 <  1:689184/140‑1 (MQ=255)

            tACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGa                                                                                                                 >  1:1492478/1‑140 (MQ=255)

            tACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGa                                                                                                                 >  1:1119199/1‑140 (MQ=255)

             aCCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGAt                                                                                                                >  2:113567/1‑140 (MQ=255)

                   cAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGtt                                                                                                          <  2:383514/140‑1 (MQ=255)

                   cAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGtt                                                                                                          >  1:1369391/1‑140 (MQ=255)

                       ggCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTAccc                                                                                                      >  1:859373/1‑140 (MQ=255)

                          cacaGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCcagc                                                                                                   <  2:65522/140‑1 (MQ=255)

                          cacaGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCcagc                                                                                                   <  2:1492478/140‑1 (MQ=255)

                          cacaGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCcagc                                                                                                   <  2:1119199/140‑1 (MQ=255)

                          cacaGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCcagc                                                                                                   <  2:828304/140‑1 (MQ=255)

                           acaGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCcagca                                                                                                  >  1:1070591/1‑140 (MQ=255)

                                ctctTAACGCCGCCCAGCGGATCAACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCt                                                                                             >  1:1036484/1‑140 (MQ=255)

                                   ttAACGCCGCCCAGCGGATCAACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTgc                                                                                          >  2:1083343/1‑140 (MQ=255)

                                         ccgccCAGCGGATCAACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATa                                                                                    >  2:709775/1‑140 (MQ=255)

                                            cccAGCGGATCAACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAg                                                                                 >  2:418870/1‑140 (MQ=255)

                                             ccAGCGGATCAACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATTAAGTTACCCAGCAGGTCTTGCAGCATaaa                                                                                  <  2:864501/138‑1 (MQ=255)

                                             ccAGCGGATCAACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGc                                                                                >  1:741779/1‑140 (MQ=255)

                                               aGCGGATCAACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCgg                                                                              <  2:1070591/140‑1 (MQ=255)

                                                         ccACGCCCTGATACAGCAGGTAGCCCCACAGACCCACTCATCCCGCAGTTCCGATGATGCCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACggg                                                                    <  2:1090724/140‑1 (MQ=255)

                                                         ccACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACggg                                                                    <  1:624831/140‑1 (MQ=255)

                                                         ccACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACggg                                                                    >  1:888434/1‑140 (MQ=255)

                                                         ccACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACggg                                                                    <  2:888434/140‑1 (MQ=255)

                                                           aCGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTa                                                                  >  1:129121/1‑140 (MQ=255)

                                                           aCGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTa                                                                  >  2:16588/1‑140 (MQ=255)

                                                             gCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAAc                                                                       >  2:649353/1‑133 (MQ=255)

                                                             gCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTAcc                                                                >  2:150454/1‑140 (MQ=255)

                                                              cccTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTAccc                                                               >  2:204600/1‑140 (MQ=255)

                                                              cccTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTAccc                                                               >  1:935033/1‑140 (MQ=255)

                                                              cccTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTAccc                                                               >  1:23801/1‑140 (MQ=255)

                                                              cccTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAAAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTAccc                                                               >  2:1426667/1‑140 (MQ=255)

                                                                 tGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTAcc                                                                <  2:1284918/136‑1 (MQ=255)

                                                                 tGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTAcc                                                                >  1:1284918/1‑136 (MQ=255)

                                                                   aTACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATc                                                          >  1:1217802/1‑140 (MQ=255)

                                                                   aTACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATc                                                          >  1:1394148/1‑140 (MQ=255)

                                                                       agcagGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAgc                                                      >  1:777437/1‑140 (MQ=255)

                                                                        gcagGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAgcg                                                     <  1:150454/140‑1 (MQ=255)

                                                                         cagGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGTAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAgcgc                                                    >  1:483681/1‑140 (MQ=255)

                                                                         cagGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAgcgc                                                    >  1:708542/1‑140 (MQ=255)

                                                                         cagGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAgcgc                                                    >  2:427367/1‑140 (MQ=255)

                                                                         cagGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAgcg                                                     <  2:117221/139‑1 (MQ=255)

                                                                         cagGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAgcg                                                     >  1:117221/1‑139 (MQ=255)

                                                                           ggTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAATGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCgg                                                  <  2:1394148/140‑1 (MQ=255)

                                                                           ggTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACggg                                                                    >  2:434066/1‑122 (MQ=255)

                                                                           ggTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCgg                                                  >  2:691216/1‑140 (MQ=255)

                                                                           ggTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCgg                                                  <  2:1249933/140‑1 (MQ=255)

                                                                           ggTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCgg                                                  <  2:1160624/140‑1 (MQ=255)

                                                                           ggTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCgg                                                  <  1:820178/140‑1 (MQ=255)

                                                                            gTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGt                                                 >  2:282731/1‑140 (MQ=255)

                                                                              aGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCgg                                                  <  1:582202/137‑1 (MQ=255)

                                                                              aGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCgg                                                  >  2:582202/1‑137 (MQ=255)

                                                                              aGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCa                                               >  2:111008/1‑140 (MQ=255)

                                                                              aGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCa                                               >  1:285833/1‑140 (MQ=255)

                                                                              aGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGAACCCGCATCCAGCGCGGTCa                                               >  1:247428/1‑140 (MQ=255)

                                                                                ccccACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAgg                                             >  1:1130545/1‑140 (MQ=255)

                                                                                ccccACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAgg                                             <  2:1039427/140‑1 (MQ=255)

                                                                                ccccACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTAACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAgg                                             <  2:1369391/140‑1 (MQ=255)

                                                                                     caGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGaa                                        >  2:516145/1‑140 (MQ=255)

                                                                                      aGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAAc                                       >  1:257038/1‑140 (MQ=255)

                                                                                       gACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACa                                      <  2:1339583/140‑1 (MQ=255)

                                                                                          ccACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAggg                                   >  1:6289/1‑140 (MQ=255)

                                                                                            acacaGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCt                                 <  1:282731/140‑1 (MQ=255)

                                                                                            acacaGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCt                                 <  2:388264/140‑1 (MQ=255)

                                                                                              acaGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTc                               <  2:1217802/140‑1 (MQ=255)

                                                                                                 gCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGaa                            <  2:935033/140‑1 (MQ=255)

                                                                                                 gCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGaa                            <  1:16588/140‑1 (MQ=255)

                                                                                                 gCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGaa                            <  2:285833/140‑1 (MQ=255)

                                                                                                  cccGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGa                                         <  2:614426/126‑1 (MQ=255)

                                                                                                  cccGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGaa                            >  2:934948/1‑139 (MQ=255)

                                                                                                  cccGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGaa                            <  1:430435/139‑1 (MQ=255)

                                                                                                  cccGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGaa                            >  2:430435/1‑139 (MQ=255)

                                                                                                  cccGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAAc                           >  2:501149/1‑140 (MQ=255)

                                                                                                  cccGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAAc                           >  1:897235/1‑140 (MQ=255)

                                                                                                   ccGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACa                          <  1:521891/140‑1 (MQ=255)

                                                                                                   ccGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACa                          <  1:709775/140‑1 (MQ=255)

                                                                                                   ccGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACa                          <  1:1426667/140‑1 (MQ=255)

                                                                                                    cGCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGaa                            <  1:382682/137‑1 (MQ=255)

                                                                                                     gCAGTACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGa                        <  2:741779/140‑1 (MQ=255)

                                                                                                         tACCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATAc                    <  2:897235/140‑1 (MQ=255)

                                                                                                          aCCGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATAcc                   <  2:262024/140‑1 (MQ=255)

                                                                                                            cGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGaa                            >  1:1179677/1‑129 (MQ=255)

                                                                                                            cGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGaa                            <  2:1179677/129‑1 (MQ=255)

                                                                                                            cGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGaa                            >  2:182886/1‑129 (MQ=255)

                                                                                                            cGATGATACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGa                 >  2:263251/1‑140 (MQ=255)

                                                                                                               tgatACCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGt              <  1:516145/140‑1 (MQ=255)

                                                                                                                   aCCGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATa          >  1:380809/1‑140 (MQ=255)

                                                                                                                     cGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATAcc        >  2:113965/1‑140 (MQ=255)

                                                                                                                     cGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATAcc        >  1:404460/1‑140 (MQ=255)

                                                                                                                     cGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATAcc        >  1:495419/1‑140 (MQ=255)

                                                                                                                     cGGCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATAcc        <  2:129121/140‑1 (MQ=255)

                                                                                                                      ggCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCa       <  1:427367/140‑1 (MQ=255)

                                                                                                                      ggCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCa       <  1:163378/140‑1 (MQ=255)

                                                                                                                      ggCAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCa       <  1:932273/140‑1 (MQ=255)

                                                                                                                        cAACCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGa     >  2:1260360/1‑140 (MQ=255)

                                                                                                                          aCCAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAg   >  2:542174/1‑140 (MQ=255)

                                                                                                                           ccAGAGAATCGG‑TTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGc  <  1:113567/140‑1 (MQ=255)

TCTGGTTGGAGATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCAACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGC  >  NC_000913/4589557‑4589820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`