Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,606,094	(G)8→7	noncoding (72/87 nt)	leuV ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,606,087	0	G	.	98.4%	258.7 / ‑1.9	65	noncoding (79/87 nt)	leuV	tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base . (31/32);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

CACAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGA  >  NC_000913/4605948‑4606221                                                                                                                                            |                                                                                                                                        cacaAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAg                                                                                                                                         <  2:1405488/140‑1 (MQ=255)    aaaCAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAgggg                                                                                                                                      <  2:188028/140‑1 (MQ=255)     aaCAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAggggg                                                                                                                                     <  2:580356/140‑1 (MQ=255)     aaCAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAggggg                                                                                                                                     <  1:1248006/140‑1 (MQ=255)     aaCAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAggggg                                                                                                                                     <  1:244545/140‑1 (MQ=255)        aGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAggggggga                                                                                                                                  >  1:330040/1‑139 (MQ=255)        aGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAggggggga                                                                                                                                  >  2:474140/1‑139 (MQ=255)          ttGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAgggggggact                                                                                                                                >  1:1453945/1‑137 (MQ=255)           tGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAgggggggactt                                                                                                                               <  1:1258681/140‑5 (MQ=255)            gCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAgggg                                                                                                                                      >  1:1016856/1‑132 (MQ=255)            gCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAgggg                                                                                                                                      <  2:1016856/132‑1 (MQ=255)            gCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTg                                                                                                                             >  1:1209753/1‑140 (MQ=255)             cGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGa                                                                                                                            <  1:484286/140‑1 (MQ=255)               gCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAAc                                                                                                                          <  2:1209753/140‑1 (MQ=255)                cATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAAcc                                                                                                                         <  2:100552/140‑1 (MQ=255)                    aCATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCa                                                                                                                     <  2:129341/140‑1 (MQ=255)                    aCATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCa                                                                                                                     <  1:176501/140‑1 (MQ=255)                          tGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCg                                                                                                               >  2:1367830/1‑140 (MQ=255)                            cTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTa                                                                                                             >  1:643112/1‑140 (MQ=255)                                  tCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGaca                                                                                                       >  1:1390983/1‑140 (MQ=255)                                    gTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑t                                                                                                    <  1:1367830/140‑1 (MQ=255)                                    cggCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑t                                                                                                    <  1:747525/138‑1 (MQ=255)                                            cACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGt                                                                                                              >  2:1366555/1‑123 (MQ=255)                                            cACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGt                                                                                                              <  1:1366555/123‑1 (MQ=255)                                            cACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTg                                                                                            >  2:1271827/1‑140 (MQ=255)                                             aCTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGa                                                                                           >  1:810267/1‑140 (MQ=255)                                                tATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGG‑‑CTTGAACCCCCACGTCCGTAAGGACCC‑TTACCCCTTAAACt                                                                                       >  2:901014/1‑140 (MQ=38)                                                 ttttcTGGCCTCATGCTGATGATAATGAATTTTAATTAGGGGTGAAAAAACAAAGAAGAAAAAAAAAAAGAAGGAAAAAAAATTGGTGCGAGGGGGGGG‑GTTGAACCCCCCCGTCCGTAAAGACAC‑TAACACCTGAAACt                                                                                       >  2:747525/5‑140 (MQ=255)                                                 aTTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCt                                                                                       >  1:1060018/1‑140 (MQ=255)                                                  ttGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTa                                                                                      <  2:1453945/140‑1 (MQ=255)                                                       ggCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGcgcg                                                                                 <  1:474140/140‑1 (MQ=255)                                                         catcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGCCTTGAACCCCCAC‑TCCGGAAAGACCCTTAAAACCT‑AAGCTAggggggct                                                                              >  1:1346691/1‑133 (MQ=255)                                                           tcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCG‑CGGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTa                                                                             <  2:1060018/140‑1 (MQ=255)                                                              tGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCa                                                                          <  2:330040/140‑1 (MQ=255)                                                                    tgatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                    >  2:336324/1‑140 (MQ=255)                                                                     gatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                   >  2:1394484/1‑140 (MQ=255)                                                                     gatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                   <  2:810267/140‑1 (MQ=255)                                                                      atAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                                                                  >  2:379378/1‑140 (MQ=255)                                                                       tAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                                 >  1:362866/1‑140 (MQ=255)                                                                        aaTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                                                                >  2:873252/1‑140 (MQ=255)                                                                             aTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACAGCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                                           <  1:699306/140‑1 (MQ=255)                                                                             aTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                                           <  1:1161881/140‑1 (MQ=255)                                                                             aTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                                           >  1:57113/1‑140 (MQ=255)                                                                               ttGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGca                                                         <  2:793087/140‑1 (MQ=255)                                                                               ttGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGca                                                         <  1:116588/140‑1 (MQ=255)                                                                                 gAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcaca                                                       >  2:675170/1‑140 (MQ=255)                                                                                 gAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcaca                                                       <  2:57113/140‑1 (MQ=255)                                                                                  aaTCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAg                                                      >  2:197915/1‑140 (MQ=255)                                                                                  aaTCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAg                                                      <  1:1394484/140‑1 (MQ=255)                                                                                  aaTCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAg                                                      >  2:1207458/1‑140 (MQ=255)                                                                                      aGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCAt                                                  >  1:1189407/1‑140 (MQ=255)                                                                                      aGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCAt                                                  >  1:1307778/1‑140 (MQ=255)                                                                                        ggATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCt                                                >  2:762309/1‑140 (MQ=255)                                                                                                  tCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACttttttt                                      >  1:147905/1‑140 (MQ=255)                                                                                                     aGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGat                                   <  1:762309/140‑1 (MQ=255)                                                                                                      ggAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGata                                  >  1:327680/1‑140 (MQ=255)                                                                                                        aagaaACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATc                                <  2:1128139/140‑1 (MQ=255)                                                                                                          gaaACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGc                              >  2:35642/1‑140 (MQ=255)                                                                                                          gaaACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGc                              >  2:2161/1‑140 (MQ=255)                                                                                                           aaaCAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGcc                             >  2:308247/1‑140 (MQ=255)                                                                                                            aaCAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAA‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCt                            >  1:430375/1‑140 (MQ=255)                                                                                                                aGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGtt                        <  1:873252/140‑1 (MQ=255)                                                                                                                 gAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGc                              <  1:451328/133‑1 (MQ=255)                                                                                                                 gAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGc                              >  2:451328/1‑133 (MQ=255)                                                                                                                     ggAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTg                   <  1:35642/140‑1 (MQ=255)                                                                                                                       aaGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCg                 <  2:1307778/140‑1 (MQ=255)                                                                                                                       aaGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCg                 <  1:379378/140‑1 (MQ=255)                                                                                                                        aGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGa                <  1:1207458/140‑1 (MQ=255)                                                                                                                        aGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGa                <  1:1271827/140‑1 (MQ=255)                                                                                                                        aGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGa                <  1:308247/140‑1 (MQ=255)                                                                                                                         gTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAg               >  1:799602/1‑140 (MQ=255)                                                                                                                              gATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggggg          <  2:327680/140‑1 (MQ=255)                                                                                                                                 aaTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGa       >  1:324610/1‑140 (MQ=255)                                                                                                                                   ttGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACt     <  1:1532592/140‑1 (MQ=255)                                                                                                                                      gTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggggg          >  2:582174/1‑132 (MQ=255)                                                                                                                                      gTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggggg          <  1:582174/132‑1 (MQ=255)                                                                                                                                      gTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGa  <  1:675170/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                        CACAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC‑TAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGA  >  NC_000913/4605948‑4606221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑