Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	759,527	(A)6→5	coding (822/2802 nt)	sucA →	subunit of E1(0) component of 2‑oxoglutarate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	759,522	0	A	.	100.0%	341.7 / NA	82	coding (817/2802 nt)	sucA	subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (42/40);  total (42/40)

TCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGT  >  NC_000913/759385‑759657                                                                                                                                          |                                                                                                                                        tctcGCTGGAAGGCGGTGTCGCGTTAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTaaa                                                                                                                                       <  1:827414/140‑1 (MQ=255) tctcGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTaaa                                                                                                                                       <  1:1503210/140‑1 (MQ=255)   tcGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTaaaaa                                                                                                                                     >  1:1387236/1‑140 (MQ=255)       tGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTaaaaaccgc                                                                                                                                 <  1:1341365/140‑5 (MQ=255)               ggTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTg                                                                                                                        >  1:929180/1‑140 (MQ=255)                 tGACGCGTTAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGtt                                                                                                                      >  1:997574/1‑140 (MQ=255)                  gACGCGTTAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTc                                                                                                                     <  2:200439/140‑1 (MQ=255)                   aCGCGTTAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTcg                                                                                                                    >  1:854835/1‑140 (MQ=255)                       gTTAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTcgacga                                                                                                                >  2:572208/1‑140 (MQ=255)                          aaTCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGtt                                                                                                             <  2:1244153/140‑1 (MQ=255)                          aaTCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGtt                                                                                                             <  1:835527/140‑1 (MQ=255)                           aTCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTc                                                                                                            <  1:1525451/140‑1 (MQ=255)                            tCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCg                                                                                                           <  2:854835/140‑1 (MQ=255)                            tCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCg                                                                                                           <  2:1532436/140‑1 (MQ=255)                                 aTGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGt                                                                                                      <  2:586927/140‑1 (MQ=255)                                  tGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTa                                                                                                     >  2:145326/1‑140 (MQ=255)                                  tGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTa                                                                                                     >  1:398439/1‑140 (MQ=255)                                   gCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTaa                                                                                                    <  1:145326/140‑1 (MQ=255)                                     ttAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAAc                                                                                                  <  2:848211/140‑1 (MQ=255)                                      tAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACa                                                                                                 <  1:202925/140‑1 (MQ=255)                                      tAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACa                                                                                                 <  2:482075/140‑1 (MQ=255)                                      tAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACa                                                                                                 >  2:178184/1‑140 (MQ=255)                                       aaaGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACAt                                                                                                <  2:1387236/140‑1 (MQ=255)                                             atgatCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGaa                                                                                          >  1:90432/1‑140 (MQ=255)                                             atgatCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGaa                                                                                          >  1:771964/1‑140 (MQ=255)                                             atgatCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGaa                                                                                          <  1:1141131/140‑1 (MQ=255)                                              tgatCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTaa                                                                                                    >  2:811390/1‑129 (MQ=255)                                              tgatCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTaa                                                                                                    <  1:811390/129‑1 (MQ=255)                                              tgatCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGaa                                                                                          >  1:572010/1‑139 (MQ=255)                                              tgatCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAac                                                                                         >  1:889635/1‑140 (MQ=255)                                              tgacCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGag                                                                                          <  2:572010/139‑2 (MQ=255)                                               gatCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAaca                                                                                        >  2:1252623/1‑140 (MQ=255)                                               gatCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAaca                                                                                        >  1:1179479/1‑140 (MQ=255)                                                   cGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCACAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTc                                                                                    >  1:215928/1‑140 (MQ=255)                                                     ccACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCgg                                                                                  >  2:265213/1‑140 (MQ=255)                                                     ccACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCgg                                                                                  >  2:1157487/1‑140 (MQ=255)                                                     ccACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCgg                                                                                  >  1:848794/1‑140 (MQ=255)                                                     ccACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCgg                                                                                  >  1:863965/1‑140 (MQ=255)                                                           tGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACggg                                                                            <  1:265213/140‑1 (MQ=255)                                                             gCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAGCATAAAGAACACCTCGGCACGGGTg                                                                          >  1:1287397/1‑140 (MQ=255)                                                             gCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTg                                                                          <  1:572208/140‑1 (MQ=255)                                                              cAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGa                                                                         <  1:714347/140‑1 (MQ=255)                                                                aCAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACg                                                                       <  1:1312418/140‑1 (MQ=255)                                                                   gCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGa                                                                    <  1:660648/140‑1 (MQ=255)                                                                    cGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGaa                                                                   >  2:1312314/1‑140 (MQ=255)                                                                     ggCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGaaa                                                                  >  1:1444396/1‑140 (MQ=255)                                                                     ggCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGaaa                                                                  >  1:74125/1‑140 (MQ=255)                                                                      gCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAAt                                                                 <  2:929180/140‑1 (MQ=255)                                                                       cACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTg                                                                          >  2:933340/1‑130 (MQ=255)                                                                       cACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTg                                                                          <  1:933340/130‑1 (MQ=255)                                                                       cACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATa                                                                >  1:1035012/1‑140 (MQ=255)                                                                          ccGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACca                                                             <  2:848794/140‑1 (MQ=255)                                                                            gcgAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACcaca                                                           <  2:771964/140‑1 (MQ=255)                                                                              gAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGaaa                                                                  <  2:1142201/131‑1 (MQ=255)                                                                              gAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGaaa                                                                  >  1:1142201/1‑131 (MQ=255)                                                                                    gTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTtc                                                   >  2:167788/1‑140 (MQ=255)                                                                                          gggATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCt                                             <  1:131228/140‑1 (MQ=255)                                                                                            gATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGa                                           >  2:307286/1‑140 (MQ=255)                                                                                              tGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACt                                         >  2:437042/1‑140 (MQ=255)                                                                                               ggCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACtt                                        >  2:128805/1‑140 (MQ=255)                                                                                                 cgcACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGTAATACCACATGGGCTTCTCGTCTGACTTcc                                      >  2:939191/1‑140 (MQ=255)                                                                                                     ccGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGATTTCCAGAc                                  <  2:74125/140‑1 (MQ=255)                                                                                                     ccGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGAc                                  <  2:1444396/140‑1 (MQ=255)                                                                                                      cGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCt                                             <  2:1496883/128‑1 (MQ=255)                                                                                                      cGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCt                                             >  1:1496883/1‑128 (MQ=255)                                                                                                      cGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGAcc                                 >  1:1260275/1‑140 (MQ=255)                                                                                                      cGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGAcc                                 >  1:148875/1‑140 (MQ=255)                                                                                                        tGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGa                               >  1:744338/1‑140 (MQ=255)                                                                                                          gtcgtcTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATg                             <  2:997574/140‑1 (MQ=255)                                                                                                           tcgtcTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATgg                            <  2:863965/140‑1 (MQ=255)                                                                                                             gtcTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATggcg                          <  2:889635/140‑1 (MQ=255)                                                                                                              tcTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATggcgg                         >  1:220973/1‑140 (MQ=255)                                                                                                              tcTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATggcgg                         <  2:808840/140‑1 (MQ=255)                                                                                                               cTGAACGTGCTGGTGAACGTGCTGGGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATggcggc                        <  1:1312314/140‑1 (MQ=255)                                                                                                                    cgtgctggtgaacgtgctggGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGt                   >  1:421569/1‑140 (MQ=255)                                                                                                                         tggtgaacgtgctggGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTgg                    <  1:267324/134‑1 (MQ=255)                                                                                                                         tggtgaacgtgctggGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTgg                    >  2:267324/1‑134 (MQ=255)                                                                                                                            tgaacgtgctggGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCa                <  2:564781/135‑1 (MQ=255)                                                                                                                            tgaacgtgctggGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCa                >  1:564781/1‑135 (MQ=255)                                                                                                                            tgaacgtgctggGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACctgg           >  2:776855/1‑140 (MQ=255)                                                                                                                             gaacgtgctggGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACctggc          >  1:1212532/1‑140 (MQ=255)                                                                                                                                cgtgctggGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACctggcgct       <  2:220973/140‑1 (MQ=255)                                                                                                                                cgtgctggGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACctggcgct       <  2:398439/140‑1 (MQ=255)                                                                                                                                 gtgctggGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACctggcgctg      <  2:421569/140‑1 (MQ=255)                                                                                                                                 gtgctggGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACctggcgctg      <  2:1035012/140‑1 (MQ=255)                                                                                                                                  tgctggGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACctggcgctgg     >  2:829693/1‑140 (MQ=255)                                                                                                                                     tggGT‑AAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGt  <  1:307286/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        TCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGT  >  NC_000913/759385‑759657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑