Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	925,850	(A)8→7	intergenic (+310/+34)	clpA → / ← serW	ATP‑dependent Clp protease ATP‑binding subunit ClpA/tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	925,843	0	A	.	100.0%	221.7 / NA	57	intergenic (+303/+41)	clpA/serW	ATP‑dependent Clp protease ATP‑binding subunit ClpA/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (27/30);  total (27/30)

GTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCA  >  NC_000913/925712‑925972                                                                                                                                    |                                                                                                                                   gTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGaaaaaaagc                                                                                                                            <  1:414627/140‑3 (MQ=255)         ttattaACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACtt                                                                                                                   <  1:506853/140‑1 (MQ=255)            ttaACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCg                                                                                                                >  1:853044/1‑140 (MQ=255)                 aacGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGa                                                                                                           >  2:858699/1‑140 (MQ=255)                 aacGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGa                                                                                                           >  1:22158/1‑140 (MQ=255)                 aacGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGa                                                                                                           >  1:471182/1‑140 (MQ=255)                  acGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCCCCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAg                                                                                                          <  1:836649/140‑1 (MQ=255)                    gggCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGct                                                                                                        <  2:52505/140‑1 (MQ=255)                    gggCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGct                                                                                                        <  1:858699/140‑1 (MQ=255)                    gggCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGct                                                                                                        <  1:424965/140‑1 (MQ=255)                      gCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACt                                                                                                                    >  2:95436/1‑126 (MQ=255)                      gCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACt                                                                                                                    <  1:95436/126‑1 (MQ=255)                      gCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGctct                                                                                                      >  2:642500/1‑140 (MQ=255)                      gCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGctct                                                                                                      >  1:1373083/1‑140 (MQ=255)                      gCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGctct                                                                                                      >  1:508782/1‑140 (MQ=255)                      gCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGctct                                                                                                      >  1:746643/1‑140 (MQ=255)                                tagtaTTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAata                                                                                            >  2:1448207/1‑140 (MQ=255)                                tagtaTTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACT‑CACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAatat                                                                                           >  2:120455/1‑140 (MQ=255)                                 agtaTTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAatat                                                                                           <  1:1252999/140‑1 (MQ=255)                                     tttGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCg                                                                                       <  2:1314733/140‑1 (MQ=255)                                       tGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGt                                                                                     <  2:678706/140‑1 (MQ=255)                                       tGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGt                                                                                     <  2:1373083/140‑1 (MQ=255)                                       tGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGt                                                                                     >  1:1325972/1‑140 (MQ=255)                                       tGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGt                                                                                     >  2:372756/1‑140 (MQ=255)                                        gAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTg                                                                                    >  1:1200230/1‑140 (MQ=255)                                          gTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑g                                                                                 >  1:601174/1‑140 (MQ=255)                                             cACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑gggg                                                                              >  1:798282/1‑140 (MQ=255)                                             cACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑gggg                                                                              >  2:29428/1‑140 (MQ=255)                                             cACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑gggg                                                                              <  2:601174/140‑1 (MQ=255)                                                tGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑ggggggg                                                                           >  1:569190/1‑140 (MQ=255)                                                   cGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGAtt                                                                        <  1:642500/140‑1 (MQ=255)                                                      aCAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGa                                                                     <  2:569190/140‑1 (MQ=255)                                                       cAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGaa                                                                    <  2:787115/140‑1 (MQ=255)                                                        aGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAAc                                                                   <  1:372756/140‑1 (MQ=255)                                                           cAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAAcccc                                                                <  2:798282/140‑1 (MQ=255)                                                                  gAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACg                                                         <  2:471182/140‑1 (MQ=255)                                                                    aaTTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGtt                                                       <  2:1325972/140‑1 (MQ=255)                                                                    aaTTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGtt                                                       <  2:508782/140‑1 (MQ=255)                                                                             gTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATac                                              <  1:121820/140‑1 (MQ=255)                                                                             gTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑AGGGGGGGGTCGGACCCCCCCCACGCTGCCGtataa                                              >  2:783388/1‑139 (MQ=25)                                                                              tAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATaca                                             <  1:475260/140‑1 (MQ=255)                                                                                aCATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATacaca                                           <  2:746643/140‑1 (MQ=255)                                                                                       ccACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGGTCGAACCCCCCACACCGTGCCGTGTACAAAAttttc                                     >  1:1354903/1‑138 (MQ=21)                                                                                       ccACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTGAACCCCCCCTACGTTTCCGTATAAACACTTTccc                                    >  2:475260/1‑139 (MQ=21)                                                                                          cccGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTGAACCCCCCCCACGTTGCCGTATAAACACCTTTCCGGc                                 >  2:694783/1‑140 (MQ=21)                                                                                          cccGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTAA‑GGGGGGGGTTTGGACCCCCCCCACCTTGCCGTATTAACACatttctagc                                 >  2:75783/1‑134 (MQ=21)                                                                                           ccGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTCGAACCCCCCCCACCTTTCCCGATACAAACTTTCCAGGCg                                >  2:77457/1‑140 (MQ=18)                                                                                             gCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTGAACCCCCCCTACGTTGGCCGATACACACCTTTCACGCGTg                              >  1:554180/1‑140 (MQ=18)                                                                                             gCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTg                              <  1:29428/140‑1 (MQ=255)                                                                                              cccACCATGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTg                              <  1:841776/140‑1 (MQ=255)                                                                                                     tGTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGGTTGGACCCCCCACACGTTGCCGTATACAAACCTTCCCGGCGGGCTCCTTc                       >  2:841776/1‑140 (MQ=18)                                                                                                      gTTCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAg                     <  1:120455/140‑1 (MQ=255)                                                                                                       ttCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGc                    <  1:77457/140‑1 (MQ=255)                                                                                                       ttCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGc                    <  1:591957/140‑1 (MQ=255)                                                                                                        tCACTGCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGcc                   <  1:783388/140‑1 (MQ=255)                                                                                                             gCCGTACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTGAAACCCCCATACGTTGCCGTATACAAACCTTTCCGGCGGGGTCCTTCCGCCAcccc              >  2:121696/1‑137 (MQ=17)                                                                                                                 tACAGACAGATAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGaca          <  1:800776/140‑1 (MQ=255)                                                                                                                         gaTAAAATGCG‑AAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCa  <  1:1079809/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                   GTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCA  >  NC_000913/925712‑925972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑