BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	136,457	(T)_8→7	coding (8/867 nt)	speE ←	spermidine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	136,450	0	T	.	100.0%	402.5 / NA	96	coding (15/867 nt)	speE	spermidine synthase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (44/52); total (44/52)

Only 100 of 111 total aligned reads displayed.

CGCCATCCAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTA  >  NC_000913/136311‑136581

cGCCATCCAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGt                                                                                                                                     >  2:1157416/1‑140 (MQ=255)

cGCCATCCAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGt                                                                                                                                     >  1:1056708/1‑140 (MQ=255)

cGCCATCCAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGt                                                                                                                                     >  2:125371/1‑140 (MQ=255)

cGCCATCCAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGt                                                                                                                                     >  2:699630/1‑140 (MQ=255)

 gCCATCCAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGtt                                                                                                                                    >  1:466187/1‑140 (MQ=255)

 gCCATCCAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGtt                                                                                                                                    >  1:1209650/1‑140 (MQ=255)

     tCCAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGtttttt                                                                                                                                >  2:10208/1‑140 (MQ=255)

     tCCAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGtttttt                                                                                                                                >  1:183634/1‑140 (MQ=255)

     tCCAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGtttttt                                                                                                                                >  1:1159539/1‑140 (MQ=255)

      ccAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGttttttt                                                                                                                               <  2:1056708/140‑1 (MQ=255)

      ccAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGttttttt                                                                                                                               <  2:83778/140‑1 (MQ=255)

      ccAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGttttttt                                                                                                                               <  1:125371/140‑1 (MQ=255)

      ccAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTAGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGttttttt                                                                                                                               <  2:18171/140‑1 (MQ=255)

          cgcCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGtttttttcggc                                                                                                                           >  1:142066/1‑136 (MQ=255)

            ccATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCTAATCTGGTCGTGTAGCGTTTCATGCCGCTG‑TTTTTTTCGGCCa                                                                                                                        <  2:553019/140‑1 (MQ=255)

            ccATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCa                                                                                                                        <  2:1366693/140‑1 (MQ=255)

            ccATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCa                                                                                                                        <  1:835128/140‑1 (MQ=255)

            ccATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCa                                                                                                                        <  2:160242/140‑1 (MQ=255)

            ccATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCa                                                                                                                        <  1:1067829/140‑1 (MQ=255)

            ccATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCa                                                                                                                        <  1:699630/140‑1 (MQ=255)

             cATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCAt                                                                                                                       <  2:1064293/140‑1 (MQ=255)

             cATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCAt                                                                                                                       <  2:200019/140‑1 (MQ=255)

               ttACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATgg                                                                                                                     <  2:142066/140‑1 (MQ=255)

               ttACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATgg                                                                                                                     >  1:692710/1‑140 (MQ=255)

                tACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATggg                                                                                                                    <  1:1080089/140‑1 (MQ=255)

                    cgACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGa                                                                                                                <  2:183634/140‑1 (MQ=255)

                       ccAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATggg                                                                                                                    <  2:596280/133‑1 (MQ=255)

                       ccAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATggg                                                                                                                    >  1:596280/1‑133 (MQ=255)

                         aaaTGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATAcct                                                                                                           <  1:10208/140‑1 (MQ=255)

                            tGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATAcctcct                                                                                                        <  1:236461/140‑1 (MQ=255)

                            tGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATAcctcct                                                                                                        <  2:1209650/140‑1 (MQ=255)

                            tGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATAcct                                                                                                           >  2:1156880/1‑137 (MQ=255)

                            tGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATAcct                                                                                                           <  1:1156880/137‑1 (MQ=255)

                                 cGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATAcctcct                                                                                                        >  2:1469915/1‑135 (MQ=255)

                                 cGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATAcctcct                                                                                                        <  1:1469915/135‑1 (MQ=255)

                                     ctcAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCGCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAaca                                                                                               >  2:467850/1‑140 (MQ=255)

                                     ctcAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATAcctc                                                                                                          <  1:992087/129‑1 (MQ=255)

                                     ctcAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAaca                                                                                               >  2:502643/1‑140 (MQ=255)

                                       cAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCCCTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACAcc                                                                                             <  2:1364864/140‑1 (MQ=255)

                                                 cAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTaaaaaaa                                                                                   <  1:595937/140‑1 (MQ=255)

                                                    aTCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAggg                                                                                <  2:1189408/140‑1 (MQ=255)

                                                    aTCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TCTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAggg                                                                                <  1:894028/140‑1 (MQ=255)

                                                          gTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAAc                                                                          >  2:971068/1‑140 (MQ=255)

                                                           tGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACa                                                                         <  2:466187/140‑1 (MQ=255)

                                                            gATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGT‑AAAAAAGGGCGCAACata                                                                       <  1:502643/140‑1 (MQ=255)

                                                            gATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTAATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACat                                                                        <  1:1286229/140‑1 (MQ=255)

                                                             aTCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACata                                                                       <  2:1159539/140‑1 (MQ=255)

                                                              tCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACataa                                                                      >  1:311912/1‑140 (MQ=255)

                                                               cGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACataat                                                                     >  2:1492213/1‑140 (MQ=255)

                                                                 gTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAg                                                                   <  1:898267/140‑1 (MQ=255)

                                                                  tCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATTCCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGc                                                                  <  1:467850/140‑1 (MQ=255)

                                                                  tCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGgcg                                                                              <  2:1460210/128‑1 (MQ=255)

                                                                  tCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGc                                                                  >  2:1242657/1‑140 (MQ=255)

                                                                  tCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGc                                                                  >  1:880477/1‑140 (MQ=255)

                                                                       tCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACATCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACa                                                             <  1:1058364/140‑1 (MQ=255)

                                                                            aTACAGAACGTTATCTACCGCAAAGTACTGCCCGAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGAc                                                        >  1:33299/1‑140 (MQ=255)

                                                                            aTACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGAc                                                        >  1:434301/1‑140 (MQ=255)

                                                                                aGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGa                                                         >  1:740076/1‑135 (MQ=255)

                                                                                aGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGa                                                         <  2:740076/135‑1 (MQ=255)

                                                                                aGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACcgcg                                                    >  1:1048081/1‑140 (MQ=255)

                                                                                aGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACcg                                                      <  2:935537/138‑1 (MQ=255)

                                                                                aGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACcg                                                      >  1:497426/1‑138 (MQ=255)

                                                                                aGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACcg                                                      >  1:935537/1‑138 (MQ=255)

                                                                                aGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACcg                                                      <  2:497426/138‑1 (MQ=255)

                                                                                aGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTGTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACcgcg                                                    >  2:1370198/1‑140 (MQ=255)

                                                                                  aaCGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGa                                                  >  2:541603/1‑140 (MQ=255)

                                                                                   aCGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGAt                                                 >  2:370999/1‑140 (MQ=255)

                                                                                    cGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATg                                                <  2:434301/140‑1 (MQ=255)

                                                                                      ttATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCa                                              >  2:1158950/1‑140 (MQ=255)

                                                                                      ttATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCa                                              <  1:1492213/140‑1 (MQ=255)

                                                                                       tATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCAc                                             >  1:1041897/1‑140 (MQ=255)

                                                                                       tATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCAc                                             >  2:712483/1‑140 (MQ=255)

                                                                                         tCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACgg                                           >  1:756896/1‑140 (MQ=255)

                                                                                           tACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTc                                         <  1:1242657/140‑1 (MQ=255)

                                                                                            aCCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGGCCGCGGATGCACGGTCa                                        >  2:1365098/1‑140 (MQ=255)

                                                                                            aCCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCa                                        >  1:545114/1‑140 (MQ=255)

                                                                                            aCCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCa                                        >  1:115752/1‑140 (MQ=255)

                                                                                              cGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAat                                      >  1:186019/1‑140 (MQ=255)

                                                                                              cGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAat                                      >  1:1144665/1‑140 (MQ=255)

                                                                                                cAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAatat                                    >  2:109941/1‑140 (MQ=255)

                                                                                                   aGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGATGATACCTCCTTTGTTAACACCCATAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTc                                 >  2:1014707/1‑140 (MQ=255)

                                                                                                     tACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTa                               <  2:186019/140‑1 (MQ=255)

                                                                                                        tGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACaa                            <  2:311912/140‑1 (MQ=255)

                                                                                                        tGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACaa                            <  1:541603/140‑1 (MQ=255)

                                                                                                           ccAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACaaaaa                         <  2:545114/140‑1 (MQ=255)

                                                                                                            cAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAg                        <  1:281070/140‑1 (MQ=255)

                                                                                                            cAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCAATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAg                        <  2:1041897/140‑1 (MQ=255)

                                                                                                               aCTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTa                     >  2:988774/1‑140 (MQ=255)

                                                                                                               aCTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTa                     >  2:1445926/1‑140 (MQ=255)

                                                                                                               aCTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTa                     >  1:1138761/1‑140 (MQ=255)

                                                                                                                    tCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAgg                <  2:33299/140‑1 (MQ=255)

                                                                                                                    tCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAgg                >  2:1124317/1‑140 (MQ=255)

                                                                                                                     cGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAggg               <  2:1048081/140‑1 (MQ=255)

                                                                                                                          aGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGattat          <  1:1365098/140‑1 (MQ=255)

                                                                                                                             gTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGa              <  2:1205721/133‑1 (MQ=255)

                                                                                                                             gTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGa              >  1:1205721/1‑133 (MQ=255)

                                                                                                                              tttCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGa      >  2:430821/1‑140 (MQ=255)

                                                                                                                              tttCATGCCACTG‑TTTTTCTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCa                  <  2:356043/128‑1 (MQ=255)

                                                                                                                              tttCATGCCACTG‑TTTTTCTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCa                  >  1:356043/1‑128 (MQ=255)

                                                                                                                                  aTGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTa  <  2:756896/140‑1 (MQ=255)

CGCCATCCAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTA  >  NC_000913/136311‑136581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`