Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,195,341	(A)5→4	coding (219/1251 nt)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,195,337	0	A	.	100.0%	342.9 / NA	82	coding (215/1251 nt)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (40/42);  total (40/42)

TTATCCCTTACATTGAAGGTGATGGAATCGGTGTAGATGTAACCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACG  >  NC_000913/1195205‑1195468                                                                                                                                     |                                                                                                                                    ttATCCCTTACATTGAAGGTGATGGAATCGGTGTAGATGTAACCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGaaaatcca                                                                                                                              <  2:657655/140‑5 (MQ=255)   aTCCCTTACATTGAAGGTGATGGAATCGGTGTAGATGTAACCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCcaca                                                                                                                           <  2:589063/140‑1 (MQ=255)   aTCCCTTACATTGAAGGTGATGGAATCGGTGTAGATGTAACCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCcaca                                                                                                                           <  1:1297273/140‑1 (MQ=255)   aTCCCTTACATTGAAGGTGATGGAATCGGTGTAGATGTAACCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCcaca                                                                                                                           <  2:1138561/140‑1 (MQ=255)     cccTTACATTGAAGGTGATGGAATCGGTGTAGATGTAACCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCcacaca                                                                                                                         <  1:982527/140‑1 (MQ=255)     cccTTACATTGAAGGTGATGGAATCGGTGTAGATGTAACCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCcacaca                                                                                                                         <  1:95388/140‑1 (MQ=255)            aTTGAAGGTGATGGAATCGGTGTAGATGTAACCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTAt                                                                                                                  >  1:156125/1‑140 (MQ=255)              tGAAGGTGATGGAATCGGTGTAGATGTAACCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAATCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATgg                                                                                                                >  2:1490935/1‑140 (MQ=255)              tGAAGGTGATGGAATCGGTGTAGATGTAACCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATgg                                                                                                                >  2:709577/1‑140 (MQ=255)                aaGGTGATGGAATCGGTGTAGATGTAACCCCATCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTc                                                                                                              >  1:235458/1‑140 (MQ=255)                aaGGTGATGGAATCGGTGTAGATGTAACCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTc                                                                                                              >  1:1154345/1‑140 (MQ=255)                aaGGTGATGGAATCGGTGTAGATGTAACCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTc                                                                                                              >  2:230679/1‑140 (MQ=255)                aaGGTGATGGAATCGGTGTAGATGTAACCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTc                                                                                                              >  1:1120715/1‑140 (MQ=255)                     gATGGAATCGGTGTAGATGTAACCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGAc                                                                                                         >  2:1014049/1‑140 (MQ=255)                     gATGGAATCGGTGTAGATGTAACCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGAc                                                                                                         >  1:600588/1‑140 (MQ=255)                      aTGGAATCGGTGTAGATGTAACCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACg                                                                                                        >  2:422549/1‑140 (MQ=255)                       tGGAATCGGTGCAGTTGTAACCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGTAATTTACACCGGTG‑AAAATCCACACAGGTTGATGGTCACTACGt                                                                                                       >  2:98122/1‑140 (MQ=255)                          aaTCGGTGTAGATGTAACCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTg                                                                                                    >  2:157324/1‑140 (MQ=255)                            tCGGTGTAGATGTAACCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGc                                                                                                  >  1:575097/1‑140 (MQ=255)                             cGGTGTAGATGTAACCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGct                                                                                                 >  2:243917/1‑140 (MQ=255)                              ggTGTAGATGTAACCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAATCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGctg                                                                                                <  1:1490935/140‑1 (MQ=255)                              ggTGTAGATGTAACCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGctg                                                                                                <  1:831147/140‑1 (MQ=255)                              ggTGTAGATGTAACCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGctg                                                                                                <  1:709577/140‑1 (MQ=255)                               gtgtAGATGTAACCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGctgc                                                                                               >  2:301438/1‑140 (MQ=255)                                         aaCCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAAc                                                                                     >  2:241836/1‑140 (MQ=255)                                          aCCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAAct                                                                                    >  1:953195/1‑140 (MQ=255)                                          aCCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAAct                                                                                    >  2:921610/1‑140 (MQ=255)                                          aCCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAG‑GTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAActc                                                                                   >  2:1439165/1‑140 (MQ=255)                                           cctcAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAActc                                                                                   <  2:1489712/137‑1 (MQ=255)                                           ccccAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAActc                                                                                   <  2:827898/140‑1 (MQ=255)                                              cAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGa                                                                                        <  2:418821/132‑1 (MQ=255)                                              cAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGa                                                                                        >  1:418821/1‑132 (MQ=255)                                               aGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGa                                                                               >  1:42345/1‑140 (MQ=255)                                                gCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGAt                                                                              <  2:1120715/140‑1 (MQ=255)                                                gCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGAt                                                                              <  1:824389/140‑1 (MQ=255)                                                    tGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGa                                                                          <  1:921610/140‑1 (MQ=255)                                                     gCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGa                                                                          <  1:499870/139‑1 (MQ=255)                                                     gCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGa                                                                          >  2:499870/1‑139 (MQ=255)                                                     gCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGAt                                                                         >  2:948844/1‑140 (MQ=255)                                                      cTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGAtt                                                                        >  1:264852/1‑140 (MQ=255)                                                        gAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCg                                                                      <  2:1507785/140‑1 (MQ=255)                                                         aaaGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGt                                                                     <  1:230679/140‑1 (MQ=255)                                                           aGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAActc                                                                                   >  1:1357246/1‑124 (MQ=255)                                                           aGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAActc                                                                                   <  2:1357246/124‑1 (MQ=255)                                                              ggTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAata                                                                <  1:241836/140‑1 (MQ=255)                                                               gTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAatat                                                               >  2:455809/1‑140 (MQ=255)                                                                   aCGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGATGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATcgcg                                                           <  1:147373/140‑1 (MQ=255)                                                                   aCGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATcgcg                                                           <  1:455809/140‑1 (MQ=255)                                                                   aCGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATcgcg                                                           <  1:948844/140‑1 (MQ=255)                                                                       tGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGc                                                       <  2:264852/140‑1 (MQ=255)                                                                         cAGTCGAGAAAGCCTATAAAGGCGAG‑GTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCAt                                                    <  1:1439165/140‑1 (MQ=255)                                                                               agaAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAg                                               <  2:600588/140‑1 (MQ=255)                                                                                 aaaGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTNAATATCGCGTTGCCATTAAAGGt                                             <  2:1288375/140‑1 (MQ=255)                                                                                   aGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTcc                                           <  1:1283177/140‑1 (MQ=255)                                                                                      cTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCt                                        >  1:960114/1‑140 (MQ=255)                                                                                      cTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCt                                        >  2:498355/1‑140 (MQ=255)                                                                                          aaaGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGAcc                                    >  2:49109/1‑140 (MQ=255)                                                                                             ggCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACt                                 <  1:422549/140‑1 (MQ=255)                                                                                               cGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTcc                               >  2:1262596/1‑140 (MQ=255)                                                                                               cGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTcc                               >  1:1242800/1‑140 (MQ=255)                                                                                                gAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTAAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCg                              >  1:1207746/1‑140 (MQ=255)                                                                                                 aGCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCgg                             >  2:682069/1‑140 (MQ=255)                                                                                                  gCGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGt                            <  2:960114/140‑1 (MQ=255)                                                                                                   cGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGtt                           >  2:1018172/1‑140 (MQ=255)                                                                                                   cGTAAAATCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGtt                           >  2:1273926/1‑140 (MQ=255)                                                                                                       aaaTCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTtggtg                       <  1:861404/140‑1 (MQ=255)                                                                                                       aaaTCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTCATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTtggtg                       <  2:933054/140‑1 (MQ=255)                                                                                                         aTCTCCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGc                     <  1:243917/140‑1 (MQ=255)                                                                                                          tctcCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCg                    <  1:682069/140‑1 (MQ=255)                                                                                                           ctcCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCgg                   <  1:157324/140‑1 (MQ=255)                                                                                                            tcCTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGt                  >  2:874310/1‑140 (MQ=255)                                                                                                             ccTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTa                 >  1:281556/1‑140 (MQ=255)                                                                                                             ccTGGATGGAAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTa                 >  1:244043/1‑140 (MQ=255)                                                                                                                 gatggaAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTATTCg             <  2:244043/140‑1 (MQ=255)                                                                                                                 gatggaAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTATTCg             <  2:235458/140‑1 (MQ=255)                                                                                                                 gatggaAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTATTCg             <  2:281556/140‑1 (MQ=255)                                                                                                                    ggaAATTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTATTCGctc          >  1:350338/1‑140 (MQ=255)                                                                                                                       aaTTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTATTCGctctct       <  2:1242800/140‑1 (MQ=255)                                                                                                                       aaTTTACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTATTCGctctct       <  2:1154345/140‑1 (MQ=255)                                                                                                                           tACACCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAAc   >  1:1455139/1‑140 (MQ=255)                                                                                                                            acacCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACg  <  1:1262596/140‑1 (MQ=255)                                                                                                                            acacCGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACg  <  1:199508/140‑1 (MQ=255)                                                                                                                                cGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTATTCGc            <  1:1175059/126‑1 (MQ=255)                                                                                                                                cGGTG‑AAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTATTCGc            >  2:1175059/1‑126 (MQ=255)                                                                                                                                     |                                                                                                                                    TTATCCCTTACATTGAAGGTGATGGAATCGGTGTAGATGTAACCCCAGCCATGCTGAAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACG  >  NC_000913/1195205‑1195468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑