Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,153,832	Δ1 bp	coding (270/660 nt)	yghB →	DedA family protein YghB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,153,829	0	G	.	100.0%	227.6 / NA	55	coding (267/660 nt)	yghB	DedA family protein YghB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (29/26);  total (29/26)

CCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTCCTGCTAAATATCACCAGCGCGCCACCTGCATGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATT  >  NC_000913/3153693‑3153963                                                                                                                                         |                                                                                                                                       ccGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAgggc                                                                                                                                     <  2:113914/140‑2 (MQ=255)  cGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAgggcg                                                                                                                                    <  1:549813/140‑3 (MQ=255)  cGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAgggcg                                                                                                                                    <  2:582895/140‑3 (MQ=255)  cGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAgggcg                                                                                                                                    <  1:1271672/140‑3 (MQ=255)   gCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAgggcgc                                                                                                                                   <  2:296068/140‑4 (MQ=255)       cATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGGTTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGt                                                                                                                              >  1:147118/1‑140 (MQ=255)       cATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGTAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGt                                                                                                                              >  1:376668/1‑140 (MQ=255)         tttttGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTa                                                                                                                            <  1:1457562/140‑1 (MQ=255)                   gCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGGTTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCa                                                                                                                  >  1:353769/1‑140 (MQ=255)                   gCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCa                                                                                                                  <  1:1275827/140‑1 (MQ=255)                    cGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCaa                                                                                                                 <  1:848426/140‑1 (MQ=255)                      aCAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCaaaa                                                                                                               <  2:1002316/140‑1 (MQ=255)                      aCAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCaaaa                                                                                                               <  2:352946/140‑1 (MQ=255)                           ttgttgATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTg                                                                                                          <  2:1314215/140‑1 (MQ=255)                           ttggggATACTGCGAGGCGCTTTGATTGCCGAGGGGGTTATGGATTTTCTGCATTCGATTGCAATTCTGCCCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTg                                                                                                          <  2:147118/135‑1 (MQ=255)                                    cTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAggctgg                                                                                                 >  2:692688/1‑140 (MQ=255)                                       gCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGgctggctg                                                                                              >  2:113380/1‑140 (MQ=255)                                        cAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGctggctgg                                                                                             >  2:94553/1‑140 (MQ=255)                                          ggCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGca                                                                                           <  2:1239464/140‑1 (MQ=255)                                           gcgcATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGcac                                                                                          >  1:852928/1‑140 (MQ=255)                                            cgcATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGcaca                                                                                         >  2:404003/1‑140 (MQ=255)                                               tttgtttcCCCGGGGGGTTGTTGATTTTCTGCCTACGATGGCGTTTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCt                                                                                      <  2:754787/139‑1 (MQ=255)                                               attgattgCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTTGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCt                                                                                      >  2:1210071/1‑140 (MQ=255)                                                ttgattgCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCtt                                                                                     <  1:404003/140‑1 (MQ=255)                                                ttgattgCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCtt                                                                                     <  1:9122/140‑1 (MQ=255)                                                 tgattgCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTc                                                                                    <  1:1186288/140‑1 (MQ=255)                                                     tgCCCAGGGGTTTATGAATTTTCTCCCTACGATTGCTATTGTGACCGCCGTAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTCCTGc                                                                                <  2:376668/140‑1 (MQ=255)                                                     tgCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTCCTGc                                                                                <  2:296730/140‑1 (MQ=255)                                                           gggggTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCtt                                                                                     <  2:397864/129‑1 (MQ=255)                                                           gggggTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCtt                                                                                     >  1:397864/1‑129 (MQ=255)                                                             gggTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTCCTGCTAAATATc                                                                        <  1:94553/140‑1 (MQ=255)                                                             gggTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTCCTGCTAAATATc                                                                        >  1:615156/1‑140 (MQ=255)                                                             gggTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTCCTGCTAAATATc                                                                        >  1:1193323/1‑140 (MQ=255)                                                               gTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTCCTGCTAAATATCAc                                                                      <  2:615156/140‑1 (MQ=255)                                                                 tATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTCCTGCTAAATATCACCa                                                                    >  2:1294438/1‑140 (MQ=255)                                                                 tATGGATTTTCTGCCTACGATTGCGACTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCtt                                                                                     <  1:280235/123‑1 (MQ=255)                                                                 tATGGATTTTCTGCCTACGATTGCGACTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCtt                                                                                     >  2:280235/1‑123 (MQ=255)                                                                      tttttCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTCCTGCTAAATATCACCAgcgcg                                                               <  2:869188/139‑1 (MQ=255)                                                                          tCTGCCTACAATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACTGTGAAAGGCTGGCTGGCACAGCTTCCTGCTAAATATCACCAGCGCGCCAc                                                           >  1:347865/1‑140 (MQ=255)                                                                             gCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTCCTGCTAAATATCAcc                                                                     <  1:1296370/127‑1 (MQ=255)                                                                             gCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTCCTGCTAAATATCAcc                                                                     >  2:1296370/1‑127 (MQ=255)                                                                                     ttGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTCCTGCTAAATATCACCAGCGCGCCACCTGCATGTTTg                                                >  1:1436524/1‑140 (MQ=255)                                                                                      tGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTTGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTCCTGCTAAATATCACCAGCGCGCCACCTGCATGTTTGa                                               <  1:1210071/140‑1 (MQ=255)                                                                                      tGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTCCTGCTAAATATCACCAGCGCGCCACCTGCATGTTTGa                                               <  1:113380/140‑1 (MQ=255)                                                                                             cTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTCCTGCTAAATATCACCAGCGCGCCACCTGCATGTTTGACCg                                            >  1:1433085/1‑136 (MQ=255)                                                                                             cTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTCCTGCTAAATATCACCAGCGCGCCACCTGCATGTTTGACCg                                            <  2:1433085/136‑1 (MQ=255)                                                                                                aCCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTCCTGCTAAATATCACCAGCGCGCCACCTGCATGTTTGACCGCCACGGt                                     >  2:367196/1‑140 (MQ=255)                                                                                                aCCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTCCTGCTAAATATCACCAGCGCGCCACCTGCATGTTTGACCGCCACGGt                                     >  1:641267/1‑140 (MQ=255)                                                                                                  cgccgcAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTCCTGCTAAATATCACCAGCGCGCCACCTGCATGTTTGACCGCCACg                                       >  2:516405/1‑136 (MQ=255)                                                                                                  cgccgcAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTCCTGCTAAATATCACCAGCGCGCCACCTGCATGTTTGACCGCCACg                                       <  1:516405/136‑1 (MQ=255)                                                                                                   gccgcAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACAGTGAAAGGCTGGCTGGCACAGCTTCCTGCTAAATATCACCAGCGCGCCACCTGCATGTTTGACCGCCACGGTctg                                  >  2:1425642/1‑140 (MQ=255)                                                                                                     cgcAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTCCTGCTAAATATCACCAGCGCGCCACCTGCATGTTTGACCGCCACGGTctgct                                >  2:827456/1‑140 (MQ=255)                                                                                                     cgcAGCAAGTCTGGGCTGCTGACTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTCCTGCTAAATATCACCAGCGCGCCACCTGCATGTTTGACCGCCACGGTctgct                                >  1:931651/1‑140 (MQ=255)                                                                                                                  agctgctGGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTACTGCTAAATATCACCAGCGCGCCACCTGCATGTTTGACCGCCACGGTCTGCTGGCGCTgctggct                   >  2:1122363/2‑140 (MQ=255)                                                                                                                       ctgGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTCCTGCTAAATATCACCAGCGCGCCACCTGCATGTTTGACCGCCACGGTCTGCTGGCGCTgctgg                     <  1:1218205/133‑1 (MQ=255)                                                                                                                       ctgGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTCCTGCTAAATATCACCAGCGCGCCACCTGCATGTTTGACCGCCACGGTCTGCTGGCGCTgctgg                     >  2:1218205/1‑133 (MQ=255)                                                                                                                       ctgGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTCCTGCTAAATATCACCAGCGCGCCACCTGCATGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACg              >  1:1370497/1‑140 (MQ=255)                                                                                                                       ctgGCTAAGTTATATTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTCCTGCTAAATATCACCAGCGCGCCACCTGCATGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACg              >  1:1163187/1‑140 (MQ=255)                                                                                                                                 tataTTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTCCTGCTAAATATCACCAGCGCGCCACCTGCATGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCa    >  2:910057/1‑140 (MQ=255)                                                                                                                                   tatTCA‑GGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTCCTGCTAAATATCACCAGCGCGCCACCTGCATGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCAtt  <  1:976295/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       CCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATTCTGACCGCCGCAGCAAGTCTGGGCTGCTGGCTAAGTTATATTCAGGGGCGCTGGTTAGGGAATACCAAAACGGTGAAAGGCTGGCTGGCACAGCTTCCTGCTAAATATCACCAGCGCGCCACCTGCATGTTTGACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATT  >  NC_000913/3153693‑3153963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑