Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,639,057	(T)5→6	coding (227/621 nt)	yfgM ←	ancillary SecYEG translocon subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,639,052	1	.	T	100.0%	222.3 / NA	62	F78I (TTT→ATT)	yfgM	ancillary SecYEG translocon subunit
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (31/31);  total (31/31)

TTTCATCGCTCGTGTCTGCCAGCCCCTGTTGTAACTGGGCGGCAGCTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAA‑TTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCC  >  NC_000913/2638920‑2639184                                                                                                                                      |                                                                                                                                     tttCATCGCTCGTGTCTGCCAGCCCCTGTTGTAACTGGGCGGCAGCTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAA‑ttttttc                                                                                                                               <  2:596574/140‑3 (MQ=255)    cATCGCTCGTGTCTGCCAGCCCCTGTTGTAACTGGGCGGCAGCTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAA‑ttttttccgc                                                                                                                            <  2:382932/140‑6 (MQ=255)       cGCTCGTGTCTGCCAGCCCCTGTTGTAACTGGGCGGCAGCTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTccgccgc                                                                                                                          >  2:1498126/1‑140 (MQ=255)         cTCGTGTCTGCCAGCCCCTGTTGTAACTGGGCGGCAGCTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTccgccgccg                                                                                                                        >  2:222783/1‑140 (MQ=255)         cTCGTGTCTGCCAGCCCCTGTTGTAACTGGGCGGCAGCTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTccgccgccg                                                                                                                        <  1:263680/140‑1 (MQ=255)         cTCGTGTCTGCCAGCCCCTGTTGTAACTGGGCGGCAGCTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTccgccgccg                                                                                                                        >  2:262633/1‑140 (MQ=255)           cGTGTCTGCCAGCCCCTGTTGTAACTGGGCGGCAGCTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCggg                                                                                                                      <  2:1313997/140‑1 (MQ=255)           cGTGTCTGCCAGCCCCTGTTGTAACTGGGCGGCAGCTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCggg                                                                                                                      <  1:651185/140‑1 (MQ=255)             tgtCTGCCAGCCCCTGTTGTAACTGGGCGGCAGCTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGAt                                                                                                                    <  2:728440/140‑1 (MQ=255)               tCTGCCAGCCCCTGTTGTAACTGGGCGGCAGCTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGc                                                                                                                  >  2:593480/1‑140 (MQ=255)                        cccTGTTGTAACTGGGCGGCAGCTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGtt                                                                                                         >  1:1000146/1‑140 (MQ=255)                        cccTGTTGTAACTGGGCGGCAGCTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGtt                                                                                                         <  2:959820/140‑1 (MQ=255)                            gttgtAACTGGGCGGCAGCTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGcc                                                                                                     <  1:262633/140‑1 (MQ=255)                                tAACTGGGCGGCAGCTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCg                                                                                                 <  1:222783/140‑1 (MQ=255)                                      ggcggcAGCTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTc                                                                                              <  1:136984/137‑1 (MQ=255)                                      ggcggcAGCTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTc                                                                                              >  2:136984/1‑137 (MQ=255)                                      ggcggcAGCTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACt                                                                                           >  2:561236/1‑140 (MQ=255)                                       gcggcAGCTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTg                                                                                                       <  1:1384082/127‑1 (MQ=255)                                       gcggcAGCTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTg                                                                                                       >  2:1384082/1‑127 (MQ=255)                                       gcggcAGCTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTc                                                                                              <  2:521373/136‑1 (MQ=255)                                       gcggcAGCTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTc                                                                                              >  1:67265/1‑136 (MQ=255)                                       gcggcAGCTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTc                                                                                              <  2:67265/136‑1 (MQ=255)                                       gcggcAGCTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTc                                                                                              >  1:521373/1‑136 (MQ=255)                                       gcggcAGCTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTg                                                                                          >  1:482271/1‑140 (MQ=255)                                       gcggcAGCTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTg                                                                                          >  1:980870/1‑140 (MQ=255)                                       gcggcAGCTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTg                                                                                          >  2:868543/1‑140 (MQ=255)                                       gcggcAGCTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTg                                                                                          >  2:1043952/1‑140 (MQ=255)                                            aGCTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTa                                                                                     <  2:37624/140‑1 (MQ=255)                                              cTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAAc                                                                                   <  1:561236/140‑1 (MQ=255)                                              cTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAAc                                                                                   <  1:274896/140‑1 (MQ=255)                                               tttCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAAcc                                                                                  <  1:1275537/140‑1 (MQ=255)                                                        ttCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGa                                                                         <  1:1043952/140‑1 (MQ=255)                                                        ttCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGa                                                                         >  2:1275813/1‑140 (MQ=255)                                                          cATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAAc                                                                                   <  2:661461/128‑1 (MQ=255)                                                          cATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAAc                                                                                   >  1:661461/1‑128 (MQ=255)                                                          cATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATa                                                                       >  1:1469478/1‑140 (MQ=255)                                                               ttGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCaa                                                                  <  1:232621/140‑1 (MQ=255)                                                                  tCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAagag                                                               >  1:403854/1‑140 (MQ=255)                                                                      caaATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGc                                                           <  1:1020800/140‑1 (MQ=255)                                                                       aaaTTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCg                                                          <  2:403854/140‑1 (MQ=255)                                                                              tgcGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTg                                                   <  1:613192/140‑1 (MQ=255)                                                                                cgcAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTg                                                   <  2:148737/138‑1 (MQ=255)                                                                                cgcAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTg                                                   >  1:148737/1‑138 (MQ=255)                                                                                cgcAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCa                                                 >  1:169981/1‑140 (MQ=255)                                                                                  cAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGa                                               >  1:157419/1‑140 (MQ=255)                                                                                     gTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATc                                            <  1:1498126/140‑1 (MQ=255)                                                                                     gTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATc                                            <  2:482271/140‑1 (MQ=255)                                                                                              aagCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATg                                   <  2:411923/140‑1 (MQ=255)                                                                                               agCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTg                                                   <  1:737453/123‑1 (MQ=255)                                                                                               agCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTg                                                   >  2:737453/1‑123 (MQ=255)                                                                                                            tAAGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAgc                     <  2:1000146/140‑1 (MQ=255)                                                                                                              aGTATTTTTATTTTCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAgcgc                   <  1:1275813/140‑1 (MQ=255)                                                                                                                    tttattttCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAgcgc                   <  2:501478/134‑1 (MQ=255)                                                                                                                    tttattttCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAgcgc                   >  1:501478/1‑134 (MQ=255)                                                                                                                    tttattttCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCgccagcca             >  2:460216/1‑140 (MQ=255)                                                                                                                    tttattttCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCgccagcca             >  2:1492805/1‑140 (MQ=255)                                                                                                                    tttattttCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCgccagcca             >  2:1217094/1‑140 (MQ=255)                                                                                                                    tttattttCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCgccagcca             >  2:657397/1‑140 (MQ=255)                                                                                                                    tttattttCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCgccagcca             >  1:335268/1‑140 (MQ=255)                                                                                                                    tttattttCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCgccagcca             >  2:970124/1‑140 (MQ=255)                                                                                                                    tttattttCAGCAGCAAATTTTTTCCGCCGCCGGGATGATATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCgccagcca             >  2:832566/1‑140 (MQ=255)                                                                                                                      tattttCAGCAGCAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAAt           <  1:593480/140‑1 (MQ=255)                                                                                                                             agcagcAAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTgcg    >  1:786795/1‑140 (MQ=255)                                                                                                                               cagcaAATTTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGcc  <  1:1492805/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     TTTCATCGCTCGTGTCTGCCAGCCCCTGTTGTAACTGGGCGGCAGCTTTCTCTAGTTCATTTTTGTCAACAAATTGCTGCGCAAGTTCCAAAGAAGCCAGCGCACCATAAGTATTTTTATTTTCAGCAGCAAA‑TTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCC  >  NC_000913/2638920‑2639184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑