| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,682,157 | (C)7→8 | coding (589/3282 nt) | yphG ← | DUF5107 domain‑containing protein YphG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,682,150 | 1 | . | C | 98.1% | 178.1 / ‑3.4 | 53 | coding (596/3282 nt) | yphG | DUF5107 domain‑containing protein YphG |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (30/22); total (31/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01 | |||||||||||
ATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAA‑GCGGAGA‑CGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTA > NC_000913/2682014‑2682279 | ataGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAA‑GCGGAGA‑CGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑‑ccc > 2:1219999/1‑140 (MQ=255) ggaaaaaTGCCCACCCCAGGGGCGTACTCCCTTTTGTAGTAGGTGCGGGTGGGGGGGGGGAAG‑GGGGAGC‑CGGCCTTTTTGCCGTAATAAAACCCCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑‑ccccccc < 2:1070894/136‑1 (MQ=255) cACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAA‑GCGGAGA‑CGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGcc > 1:645811/1‑140 (MQ=255) cGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAA‑GCGGAGA‑CGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑‑CCCCCCCTTTCACTGCCGGGTTGGcc > 1:162022/1‑140 (MQ=255) ggAGTAGTCCTTTTTGTAGTAAGTGCGGGTAGGGGGGGGAAAG‑GGGGAGC‑CGCCCCGTTTGCCGTGATAAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGcc < 2:245813/140‑1 (MQ=255) ggAGTAGTCCTCTTCGTAGTAGGGGCGGGTAGCGGGGGGAAAA‑GCGGAGC‑CGCCCCGTTTGCCGTAATAAACCACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGcc < 2:441254/140‑1 (MQ=255) tagtCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAA‑GCGGAGA‑CGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCccacc > 1:245813/1‑140 (MQ=255) gtCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAA‑GCGGAGA‑CGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCcaccac > 1:715935/1‑140 (MQ=255) cACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAA‑GCGGAGA‑CGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACaaa > 1:552962/1‑139 (MQ=255) cACTTCGTAGTAAGTGCCGGTAGCGATGGGGAAA‑GCGGAGA‑CGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAg > 1:441254/1‑140 (MQ=255) ttcgtagtaAGTGCCGGTAGCGATGGGGAAA‑GCGGAGA‑CGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGaaa > 2:885192/4‑140 (MQ=255) agtaAGTGCCGGTAGCGATGGGGAAA‑GCGGAGA‑CGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGAcg > 2:958922/1‑140 (MQ=255) agtaAGTGCCGGTAGCGATGGGGAAA‑GCGGAGA‑CGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGAcg > 1:968287/1‑140 (MQ=255) ggTGCGGGTAGGGAGGGGAAAG‑GCGGAGC‑CGCCCCGTTTCCCGTAATCAAACCCCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACgcggc < 2:1184463/139‑1 (MQ=255) gCCGGTAGCGATGGGGAAA‑GCGGAGA‑CGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCggcgtg > 2:188265/1‑140 (MQ=255) gCCGGTAGCGATGGGGAAA‑GCGGAGA‑CGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCggcgtg > 1:794336/1‑140 (MQ=255) cGGTAGCGATGGGGAAA‑GCGGAGA‑CGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCCTTTCACTGCCGGGTTGGCGCACCACAAGAAATGACGCggcgtgg > 1:1262971/1‑140 (MQ=255) cGGTAGCGATGGGGAAA‑GCGGAGA‑CGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCggcgtggc > 2:338108/1‑140 (MQ=255) gTAGCGATGGGGAAA‑GCGGAGA‑CGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCggcgtggcgt > 1:1485026/1‑140 (MQ=255) gggggggggggAAG‑GGGGAGG‑CGCCCTTTTCGCCGTAAACAACCCCCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGtt < 2:1453551/133‑1 (MQ=255) gggggggggAAG‑GCGGAGC‑CGGCCTGTTTGCCTTAACAAACCCCCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTcc < 2:455567/135‑1 (MQ=255) gggggggggAAA‑GGGGGGGCCGGCCGTTTTGCCGTAAAAAACCCCCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTc < 2:968287/135‑1 (MQ=255) gggaggggaAAA‑GGGGAGC‑CGCCCCTTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTcc < 1:1183835/135‑1 (MQ=255) gCGATGGGGAAA‑GCGGAGA‑CGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTcc > 2:517742/1‑140 (MQ=255) ggggggggAAAGGGGGAGC‑CGGCCCGTTTGCCGTAACAAAACCCCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTcc < 2:251811/136‑1 (MQ=255) cGATGGGGAAA‑GCGGAGA‑CGGCCCGTTTGCCGTGATCAAACACCGCCGTTATATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTccc > 2:615/1‑140 (MQ=255) a‑GCGGAGA‑CGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGAcg > 2:1154544/1‑140 (MQ=255) cGGAGA‑CGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGAcgct > 1:251811/1‑139 (MQ=255) ccGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGAtt > 1:64988/1‑140 (MQ=255) ccGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCg < 1:20691/130‑1 (MQ=255) ccGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCg > 2:20691/1‑130 (MQ=255) aTCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCgccgcc < 2:1404131/140‑1 (MQ=255) gccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgc > 1:1167221/1‑140 (MQ=255) gccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgc > 1:1107821/1‑140 (MQ=255) gccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgc > 1:520234/1‑140 (MQ=255) ccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgcg < 2:794336/140‑1 (MQ=255) ccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgcg < 1:517742/140‑1 (MQ=255) cgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGTGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgcgc < 1:1219999/140‑1 (MQ=255) cgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgcgc < 2:1413340/140‑1 (MQ=255) cgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgcgc < 1:188265/140‑1 (MQ=255) gTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgcg < 2:707418/138‑1 (MQ=255) gTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgcg > 1:707418/1‑138 (MQ=255) aTCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTg < 2:715935/140‑1 (MQ=255) cggcggGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGaaa > 1:1047978/1‑140 (MQ=255) ggcggGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAAc > 1:650701/1‑140 (MQ=255) cggGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCt > 2:314373/1‑140 (MQ=255) cggGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGATCCCGTTATAGACGCGGCTGGCGATTTGCTGCGCCGCCCGGTCACGGCGCAGGGTGAAAcca > 2:858070/1‑139 (MQ=255) gAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGtc > 1:672721/1‑140 (MQ=255) aGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGtcat < 2:520234/140‑1 (MQ=255) aGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGtcat < 1:1503155/140‑1 (MQ=255) aGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGtcat < 2:1167221/140‑1 (MQ=255) gCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCAcc > 1:537446/1‑140 (MQ=255) ctctGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCt > 1:973397/1‑140 (MQ=255) tGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTa < 2:537446/140‑1 (MQ=255) tGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTa < 2:64988/140‑1 (MQ=255) | ATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAA‑GCGGAGA‑CGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTA > NC_000913/2682014‑2682279 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |