Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,101,039 (C)7→8 coding (585/720 nt) yggN ← putative EcfF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,101,0321.C96.9% 213.1 / ‑0.5 65coding (592/720 nt)yggNputative EcfF
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base C (28/35);  total (29/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

AAATAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGGCGC  >  NC_000913/3100894‑3101166
                                                                                                                                           |                                                                                                                                       
aaaTAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑c                                                                                                                                       <  2:877561/140‑1 (MQ=255)
aaaTAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑c                                                                                                                                       <  2:32861/140‑1 (MQ=255)
 aaTAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑cc                                                                                                                                      <  2:1434667/140‑1 (MQ=255)
    agagGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑ccccc                                                                                                                                   <  2:1054209/140‑1 (MQ=255)
     gagGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑cccccc                                                                                                                                  >  2:476404/1‑140 (MQ=255)
      agGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTTCAG‑‑ccccccc                                                                                                                                 >  2:1455559/1‑140 (MQ=255)
       ggATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACACGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑cccccccc                                                                                                                                >  1:1290392/1‑139 (MQ=255)
          ttattTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑ccccccccagg                                                                                                                             <  1:476404/140‑5 (MQ=255)
           tattTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑ccccccccaggc                                                                                                                            >  2:1477440/1‑135 (MQ=255)
            attTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCt                                                                                                                            <  1:768782/140‑1 (MQ=255)
             ttttAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCAGTTTGGATTGAGGATTGCAG‑‑ccccccccagg                                                                                                                             >  1:1344004/1‑133 (MQ=255)
             ttttAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCAGTTTGGATTGAGGATTGCAG‑‑ccccccccagg                                                                                                                             <  2:1344004/137‑5 (MQ=255)
                tAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTccc                                                                                                                        <  2:971596/140‑1 (MQ=255)
                  aaTTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAg                                                                                                                      >  1:422492/1‑140 (MQ=255)
                   aTTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTCCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGc                                                                                                                     <  2:1066354/140‑1 (MQ=255)
                    ttCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGca                                                                                                                    <  1:853160/140‑1 (MQ=255)
                       ccGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACAt                                                                                                                 <  2:446494/140‑1 (MQ=255)
                       ccGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACAt                                                                                                                 <  1:921264/140‑1 (MQ=255)
                       ccGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACAt                                                                                                                 <  1:1508314/140‑1 (MQ=255)
                       ccGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACAt                                                                                                                 <  2:1264564/140‑1 (MQ=255)
                       ccGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACAt                                                                                                                 >  2:1275356/1‑140 (MQ=255)
                        cGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACACGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACAtt                                                                                                                <  2:1290392/140‑1 (MQ=255)
                         gACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTc                                                                                                               <  1:682725/140‑1 (MQ=255)
                          aCCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCt                                                                                                              >  1:1419590/1‑140 (MQ=255)
                               ggCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCCAGGCTTCCCAGCACATTCTGTaa                                                                                                          >  2:634226/1‑140 (MQ=255)
                                    tGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGtt                                                                                                    >  1:510163/1‑140 (MQ=255)
                                    tGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGtt                                                                                                    >  1:419690/1‑140 (MQ=255)
                                    tGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGtt                                                                                                    >  2:192497/1‑140 (MQ=255)
                                    tGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGtt                                                                                                    >  1:731203/1‑140 (MQ=255)
                                    tGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGtt                                                                                                    >  1:743720/1‑140 (MQ=255)
                                    tGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTG‑GGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTa                                                                                                   >  1:206768/1‑140 (MQ=255)
                                           aTCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATGTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccgccg                                                                                             <  2:1335175/140‑1 (MQ=255)
                                           aTCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccgccg                                                                                             <  1:565367/140‑1 (MQ=255)
                                            tCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCt                                                                                           >  1:634285/1‑140 (MQ=255)
                                                      tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCAc                                                                                  >  2:839786/1‑140 (MQ=255)
                                                      tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCAc                                                                                  >  1:1316356/1‑140 (MQ=255)
                                                       cacaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCg                                                                                <  2:634285/140‑1 (MQ=255)
                                                        acaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCg                                                                                >  2:1497231/1‑140 (MQ=255)
                                                        acaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCg                                                                                >  1:519129/1‑140 (MQ=255)
                                                           aCGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCtt                                                                             >  2:126517/1‑140 (MQ=255)
                                                              cgGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTcg                                                                          >  1:839501/1‑140 (MQ=255)
                                                                gCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTcgcg                                                                        <  1:126517/140‑1 (MQ=255)
                                                                gCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTcgcg                                                                        <  2:422492/140‑1 (MQ=255)
                                                                gCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTcgcg                                                                        <  1:480088/140‑1 (MQ=255)
                                                                     aaaCATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt                                                                   <  2:1316356/140‑1 (MQ=255)
                                                                     aaaCATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt                                                                   <  1:192497/140‑1 (MQ=255)
                                                                      aaCATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt                                                                   <  1:634226/140‑1 (MQ=255)
                                                                         aTCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCa                                                               >  2:759296/1‑140 (MQ=255)
                                                                          tCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCAt                                                              <  2:743720/140‑1 (MQ=255)
                                                                           cTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCAtt                                                             >  2:282029/1‑140 (MQ=255)
                                                                           cTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCAt                                                              <  1:1021077/139‑1 (MQ=255)
                                                                           cTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCAt                                                              <  1:625000/139‑1 (MQ=255)
                                                                           cTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCAt                                                              >  2:625000/1‑139 (MQ=255)
                                                                           cTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCAt                                                              >  2:1021077/1‑139 (MQ=255)
                                                                              tGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTG‑GGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATg                                                         <  2:206768/140‑1 (MQ=255)
                                                                               gCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATg                                                         <  1:759296/140‑1 (MQ=255)
                                                                               gCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATg                                                         <  2:1419590/140‑1 (MQ=255)
                                                                               gCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATg                                                         <  2:510163/140‑1 (MQ=255)
                                                                                   aaCTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGt                                                     >  1:1293241/1‑140 (MQ=255)
                                                                                           gAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTaaa                                             <  1:1017598/140‑1 (MQ=255)
                                                                                               tCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTc                                         <  2:519129/140‑1 (MQ=255)
                                                                                                   tttCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCAc                                      >  1:458316/1‑140 (MQ=255)
                                                                                                    ttCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCAccc                                    <  2:1475654/140‑1 (MQ=255)
                                                                                                     tCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCa                                   <  2:839501/140‑1 (MQ=255)
                                                                                                     tCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTACCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCa                                   <  2:731203/140‑1 (MQ=255)
                                                                                                         gCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGc                               >  2:201105/1‑140 (MQ=255)
                                                                                                          cTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGcc                              >  2:276911/1‑140 (MQ=255)
                                                                                                             tttCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCCAGGCTTCCCACCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTg                            >  2:817694/1‑140 (MQ=255)
                                                                                                             tttCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGa                           <  1:1275356/140‑1 (MQ=255)
                                                                                                                                gAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCtt        <  1:839786/140‑1 (MQ=255)
                                                                                                                                gAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCtt        >  1:296403/1‑140 (MQ=255)
                                                                                                                                     ttGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGgcg   <  2:1293241/140‑1 (MQ=255)
                                                                                                                                     ttGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGgcg   <  1:1477440/140‑1 (MQ=255)
                                                                                                                                     ttGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGgcg   <  1:67027/140‑1 (MQ=255)
                                                                                                                                      tGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGgcgc  >  1:118473/1‑140 (MQ=255)
                                                                                                                                      tGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGgcgc  >  1:15042/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                       
AAATAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGGCGC  >  NC_000913/3100894‑3101166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑