Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,556,2070TC36.4% 63.2 / 14.8 44S215G (AGT→GGT) ‡rtcARNA 3'‑terminal phosphate cyclase
Reads supporting (aligned to +/- strand):  ref base T (26/2);  new base C (0/16);  total (26/18)
Fisher's exact test for biased strand distribution p-value = 3.67e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGT  >  NC_000913/3556074‑3556345
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gCACTGACGCTTTTTTCACCGACGACAAAAAAGCGTTCGGTGATTTTTTCTCTTTCGACTTCAAGCGAGACGTTATTCCCCGCCCCCTGGTCGCGCGGCAGTTTATAAATATTCTGTTCAGGCAGGAAAAAC‑CCCCCCGc                                                                                                                                      <  2:545154/140‑1 (MQ=255)
      aCGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAgtgt                                                                                                                                >  1:988562/1‑140 (MQ=255)
      aCGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAgtgt                                                                                                                                >  1:1449410/1‑140 (MQ=255)
      aCGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAgtgt                                                                                                                                >  1:199844/1‑140 (MQ=255)
      aCGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAgtgt                                                                                                                                >  2:1072697/1‑140 (MQ=255)
        gcgtTTTCCCCCGACGAAAAAAAGGCGTTCGGTAATTTTTTCTTTTTCGACTTCAAGCGAGACGGTATCCCCCGGCCCCTGGTCGCGGGGCAGTTTATAAATTTTCTTTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAg                                                                                                                              <  2:1449410/140‑1 (MQ=255)
         cgcTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGc                                                                                                                             >  2:139661/1‑140 (MQ=255)
                 cccGACGACAAAAAAGCGTTGGGTGATTTTTTCCCTTTCGACTTCAAGCGAGACGTTATTCCCCGGCCCCTGGTCGCGCGGCAGGTTATAAATTTTCTGTTCAGGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCAc                                                                                                                     <  2:1485380/139‑1 (MQ=255)
                  cggacgaaAAAAAAGCTTTGGGTGATTTTTTCCTTTTCGCCTACAAGCGAGTGGTTTTTCCCCGGCCCCTGGTCGCGCGGCGGGTTAAAAATTTTCTTTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACg                                                                                                                    <  2:1408021/138‑1 (MQ=255)
                  ccGACGACAAAAAAGCGTTGGGTGATTTTTTCACTTTCGCCTTCAAGCGAGACGGTATTCCCCGGCCCGGGGTCGCGCGGCAGGTTATAAATTTTCTGTTCAGGAAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACg                                                                                                                    <  2:778852/140‑1 (MQ=255)
                      cgacAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCa                                                                                                                >  2:417474/1‑140 (MQ=255)
                      cgacAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCa                                                                                                                >  2:370273/1‑140 (MQ=255)
                        acAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGc                                                                                                              >  1:1408021/1‑140 (MQ=255)
                        acAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGc                                                                                                              >  1:778852/1‑140 (MQ=255)
                              aaGCGTTCGGTTATTTTTTCCCTTTCGACTTCAAGGGAGACGTTATTCCCCGGCCCCTGGCCGCGGGGAAGTTTATGAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATg                                                                                                        <  2:199844/140‑1 (MQ=255)
                                        tgtttttttCATTTCCAACTTCAAGCGGGACGTTATTCCCCGGCCCCTGGCCGCGCGGCGGTTTATAATTTTTCTTTTCAGGGGGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGcaca                                                                                              <  2:1141493/135‑1 (MQ=255)
                                        tgtttttttCACTTTCGATTACAGGCGAGAGGGTATTCCCCGGCCCCTGGTGGCGGGGCAGGTTATAAATATTCTGTTCGTGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGcaca                                                                                              <  2:330097/135‑1 (MQ=255)
                                           ttttttCCCTTTCGACTTCAAGCGAGACTGTATTCCCCGCCCCCTGGCCGCGCGGCGGTTTATAAATATTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCa                                                                                           <  2:628713/138‑1 (MQ=255)
                                                 cACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAAt                                                                                     >  1:1263809/1‑140 (MQ=255)
                                                     ttCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGaa                                                                                 >  1:276836/1‑140 (MQ=255)
                                                                     cgggTTTTCCCCGCCCCCTGGCCGCGCGGCAGTTTATAAATTTTCTGTTCATG‑AGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTg                                                                 <  2:807986/138‑1 (MQ=255)
                                                                       ggTATTCCCCGCCCCCGGGTCGCGCGGCAGGTTATAAATATTCTGTTCAGGCAGGAAAAAA‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGca                                                               <  2:276836/140‑1 (MQ=255)
                                                                          tttccccGCCCCCTGGCCGCGCGGCAGTTTATAAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACaa                                                            <  2:1263809/136‑1 (MQ=255)
                                                                              cccGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGtt                                                        >  2:63160/1‑140 (MQ=255)
                                                                               cggcccccGGGCCGCGCGGCAGTTTATGAATATTCTGTTCAGGAAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTc                                                       <  2:860780/136‑1 (MQ=255)
                                                                                   ccccTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTccctg                                                   >  1:807986/1‑138 (MQ=255)
                                                                                   ccccTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgc                                                   >  2:1360011/1‑140 (MQ=255)
                                                                                   ccccTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgc                                                   >  2:221412/1‑140 (MQ=255)
                                                                                    cccTGGTCGCGCGGCGGTTTATAAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgcg                                                  <  1:370273/140‑1 (MQ=255)
                                                                                    cccTGGCCGGGCGGCAGGTTATAAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgcg                                                  <  1:417474/140‑1 (MQ=255)
                                                                                        ggTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCg                                              >  1:860780/1‑140 (MQ=255)
                                                                                         gTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGc                                             >  1:874304/1‑140 (MQ=255)
                                                                                             cgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg                                         >  2:562115/1‑140 (MQ=255)
                                                                                                 gCAGTTTATAAATATTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGc                                     <  1:218291/140‑1 (MQ=255)
                                                                                                  cAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                    <  1:1360011/140‑1 (MQ=255)
                                                                                                  aagtttATAAATATTCTGTTCAGGCAGGAAAAAC‑CCCCCCGCCAGTGGAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                    <  1:1018328/136‑1 (MQ=255)
                                                                                                               ttCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                       >  2:1502767/1‑140 (MQ=255)
                                                                                                                tCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGac                      >  1:277504/1‑139 (MQ=255)
                                                                                                                tCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt                      >  2:1003453/1‑140 (MQ=255)
                                                                                                                  ttttCAGGAAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGTTCTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGc                    <  2:1368755/138‑1 (MQ=255)
                                                                                                                    ttCATGCAGGAAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                  <  1:63160/140‑1 (MQ=255)
                                                                                                                     taaTGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCAc                 >  1:494008/3‑140 (MQ=255)
                                                                                                                       aTGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACgg               >  1:269361/1‑140 (MQ=255)
                                                                                                                        tGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACggg              >  2:495472/1‑140 (MQ=255)
                                                                                                                        tGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACggg              >  1:1087970/1‑140 (MQ=255)
                                                                                                                         gCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGc             >  2:609110/1‑140 (MQ=255)
                                                                                                                                     cTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGGGAGACTTCCGt  <  1:221412/140‑1 (MQ=255)
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GCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGT  >  NC_000913/3556074‑3556345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑