Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	383,575	A→G	Y121H (TAT→CAT)	yaiP ←	putative glucosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	383,575	0	A	G	100.0%	249.9 / NA	72	Y121H (TAT→CAT)	yaiP	putative glucosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/33);  total (39/33)

CTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATAGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGC  >  NC_000913/383441‑383707                                                                                                                                       |                                                                                                                                     cTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGgtgt                                                                                                                                 <  1:1183173/140‑1 (MQ=255)         tCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCa                                                                                                                         <  2:725792/140‑1 (MQ=255)            cgcgGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACtt                                                                                                                      >  1:1030671/1‑140 (MQ=255)            cgcgGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACtt                                                                                                                      >  1:1340906/1‑140 (MQ=255)                 aTGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTaaaaa                                                                                                                 >  1:1032771/1‑140 (MQ=255)                     gCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAAcc                                                                                                             >  2:682998/1‑140 (MQ=255)                     gCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAAcc                                                                                                             <  1:767344/140‑1 (MQ=255)                     gCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAAcc                                                                                                             <  2:385884/140‑1 (MQ=255)                          aaCAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTc                                                                                                        <  1:597985/140‑1 (MQ=255)                          aaCAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCC‑ACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCg                                                                                                       >  1:358684/1‑140 (MQ=255)                           aCAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCg                                                                                                       >  2:1311750/1‑140 (MQ=255)                             aGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCa                                                                                                     <  2:7339/140‑1 (MQ=255)                                   gcgcCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGc                                                                                               >  2:1489136/1‑140 (MQ=255)                                   gcgcCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGc                                                                                               >  2:733130/1‑140 (MQ=255)                                     gcCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGt                                                                                             >  1:387329/1‑140 (MQ=255)                                        tttCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAAt                                                                                          >  2:16285/1‑140 (MQ=255)                                         ttCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAAtt                                                                                         >  1:1324568/1‑140 (MQ=255)                                           cAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGa                                                                                       <  2:343/140‑1 (MQ=255)                                             aCGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAgg                                                                                     <  1:581718/140‑1 (MQ=255)                                              cGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGc                                                                                    >  1:1487648/1‑140 (MQ=255)                                                cAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCa                                                                                  <  2:447497/140‑1 (MQ=255)                                                 aGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCAt                                                                                 >  2:745045/1‑140 (MQ=255)                                                      agGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATtcatc                                                                            <  1:1489136/140‑1 (MQ=255)                                                        ggAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCCTTCATCAg                                                                          >  1:368948/1‑140 (MQ=255)                                                          aaTGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAgcg                                                                        >  1:82972/1‑140 (MQ=255)                                                           aTGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGa                                                                                       >  2:240010/1‑124 (MQ=255)                                                           aTGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGa                                                                                       <  1:240010/124‑1 (MQ=255)                                                            tGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGca                                                                      <  2:1487648/140‑1 (MQ=255)                                                            tGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGca                                                                      <  1:16285/140‑1 (MQ=255)                                                             gccgccTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAgg                                                                                     >  2:141058/1‑124 (MQ=255)                                                             gccgccTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAgg                                                                                     <  1:141058/124‑1 (MQ=255)                                                             gccgccTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATtcatca                                                                           >  1:678888/1‑134 (MQ=255)                                                             gccgccTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATtcatca                                                                           <  2:678888/134‑1 (MQ=255)                                                             gccgccTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGcac                                                                     >  2:713982/1‑140 (MQ=255)                                                             gccgccTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGcac                                                                     >  1:945049/1‑140 (MQ=255)                                                             gccgccTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGcac                                                                     >  1:975055/1‑140 (MQ=255)                                                             gccgccTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGcac                                                                     >  2:469502/1‑140 (MQ=255)                                                             gccgccTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGca                                                                      <  2:72672/139‑1 (MQ=255)                                                             gccgccTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGca                                                                      >  1:895811/1‑139 (MQ=255)                                                             gccgccTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGca                                                                      >  1:72672/1‑139 (MQ=255)                                                             gccgccTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGca                                                                      <  2:895811/139‑1 (MQ=255)                                                                    aCGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCttt                                                              >  1:1135354/1‑140 (MQ=255)                                                                      ggCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTAc                                                            <  1:745045/140‑1 (MQ=255)                                                                      ggCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTAc                                                            <  2:82972/140‑1 (MQ=255)                                                                        cATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCg                                                          <  2:212931/140‑1 (MQ=255)                                                                        cATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCg                                                          <  1:1224933/140‑1 (MQ=255)                                                                             gCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTAtt                                                     >  1:267076/1‑140 (MQ=255)                                                                                    gcTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGc                                              <  2:348609/140‑1 (MQ=255)                                                                                      tCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGt                                            <  2:1340906/140‑1 (MQ=255)                                                                                       cAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTg                                           <  2:368948/140‑1 (MQ=255)                                                                                        aaTTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCGCCACCTTTACCGGTATTTTTTTGCGTGa                                          <  2:1135354/140‑1 (MQ=255)                                                                                        aaTTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGa                                          <  2:945049/140‑1 (MQ=255)                                                                                        aaTTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGa                                          <  2:1188698/140‑1 (MQ=255)                                                                                         aTTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGAc                                         <  2:1324568/140‑1 (MQ=255)                                                                                                  aGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGACGGCAACaaa                                >  2:770138/1‑140 (MQ=255)                                                                                                          gCCCATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGACGGCAACAAAGCGGTCGc                        >  1:573020/1‑140 (MQ=255)                                                                                                           cccATTCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGACGGCAACAAAGCGGTCGcc                       <  1:713982/140‑1 (MQ=255)                                                                                                               ttCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCAt                   >  2:755024/1‑140 (MQ=255)                                                                                                               ttCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCAt                   >  2:71937/1‑140 (MQ=255)                                                                                                                tCCGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCAtt                  >  2:1248201/1‑140 (MQ=255)                                                                                                                  cGTCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTg                <  1:682998/140‑1 (MQ=255)                                                                                                                    tCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCg              >  1:1052446/1‑140 (MQ=255)                                                                                                                    tCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCg              >  2:1308726/1‑140 (MQ=255)                                                                                                                    tCTTGATCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCg              >  1:465287/1‑140 (MQ=255)                                                                                                                         aTCGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGa         >  1:1036465/1‑140 (MQ=255)                                                                                                                           cGGGCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACt       <  2:387329/140‑1 (MQ=255)                                                                                                                             ggCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTc     <  1:71937/140‑1 (MQ=255)                                                                                                                              gCGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTcc    >  2:1313352/1‑140 (MQ=255)                                                                                                                               cGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCg   >  1:1386095/1‑140 (MQ=255)                                                                                                                               cGGAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGACGGCAACAAAGCGGCCGCCCCATTTGCGTTTGACTTCCg   >  2:525861/1‑140 (MQ=255)                                                                                                                                ggAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGc  <  1:504724/140‑1 (MQ=255)                                                                                                                                ggAACATGGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGc  >  2:504724/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                     CTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATAGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGC  >  NC_000913/383441‑383707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑