Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,622,367	T→C	V1059A (GTC→GCC)	rhsB →	rhs element protein RhsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,622,367	0	T	C	100.0%	191.1 / NA	56	V1059A (GTC→GCC)	rhsB	rhs element protein RhsB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/28);  total (28/28)

GCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACC  >  NC_000913/3622232‑3622492                                                                                                                                        |                                                                                                                              gCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGaa                                                                                                                           <  2:177206/140‑1 (MQ=255) gCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGaa                                                                                                                           >  1:64670/1‑140 (MQ=255)    ggATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACt                                                                                                                        >  2:1506452/1‑140 (MQ=255)      aTGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGTGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCAAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGc                                                                                                                      <  2:135782/140‑1 (MQ=255)       tGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGcc                                                                                                                     >  1:333831/1‑140 (MQ=255)           gCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCg                                                                                                                 >  2:381440/1‑140 (MQ=255)           gCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCg                                                                                                                 <  2:1123864/140‑1 (MQ=255)             tGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGt                                                                                                               <  1:1234918/140‑1 (MQ=255)               tCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGa                                                                                                             >  2:467782/1‑140 (MQ=255)                           cAAGTGACCTGGTACGGCTGGGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAAc                                                                                                 >  1:1273571/1‑140 (MQ=255)                             aGTGACCTGGTACGGCTGGGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGc                                                                                               >  1:575024/1‑140 (MQ=255)                              gTGACCTGGTACGGCTGGGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCa                                                                                              <  1:694313/140‑1 (MQ=255)                                    tGGTACGGCTGGGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGcgccg                                                                                        <  2:1451452/140‑1 (MQ=255)                                        aCGGCTGGGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGc                                                                                    <  2:575024/140‑1 (MQ=255)                                          ggCTGGGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCt                                                                                  <  2:1198980/140‑1 (MQ=255)                                            cTGGGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTgg                                                                                >  2:292503/1‑140 (MQ=255)                                            cTGGGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTgg                                                                                >  2:1362168/1‑140 (MQ=255)                                                gACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGa                                                                            <  2:433133/140‑1 (MQ=255)                                                gACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGa                                                                            >  2:1521548/1‑140 (MQ=255)                                                gACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGa                                                                            <  1:381440/140‑1 (MQ=255)                                                gACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGa                                                                            <  1:1309139/140‑1 (MQ=255)                                                  cGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATg                                                                          <  2:487606/140‑1 (MQ=255)                                                     cGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGccc                                                                       >  2:395484/1‑140 (MQ=255)                                                     cGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGccc                                                                       >  2:1398834/1‑140 (MQ=255)                                                          ggCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGACCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTcag                                                                  <  1:1237924/140‑1 (MQ=255)                                                           gCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTcagc                                                                 >  1:163192/1‑140 (MQ=255)                                                                 cACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCcg                                                           <  1:1398834/140‑1 (MQ=255)                                                                  aCGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCGGCAGTCcgg                                                          <  1:467782/140‑1 (MQ=255)                                                                    gATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCcggcg                                                        >  1:253462/1‑140 (MQ=255)                                                                       aCAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCTGgcggcg                                                     <  2:1273571/140‑1 (MQ=255)                                                                              gACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGa                                                 >  2:863355/1‑137 (MQ=255)                                                                              gACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGa                                                 <  1:863355/137‑1 (MQ=255)                                                                              gACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACgg                                              <  1:1512154/140‑1 (MQ=255)                                                                              gACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACgg                                              <  2:163192/140‑1 (MQ=255)                                                                              gACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACgg                                              >  1:1125048/1‑140 (MQ=255)                                                                                cAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTg                                            >  1:1278637/1‑140 (MQ=255)                                                                                cAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTg                                            >  2:388800/1‑140 (MQ=255)                                                                                 aGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTgg                                           <  1:1506452/140‑1 (MQ=255)                                                                                 aGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTgg                                           <  1:388800/140‑1 (MQ=255)                                                                                  ggAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGc                                          >  1:709415/1‑140 (MQ=255)                                                                                   gAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCa                                         <  1:1521548/140‑1 (MQ=255)                                                                                     gCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAgt                                       >  1:1009796/1‑140 (MQ=38)                                                                                         cATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGa                                                 >  2:731880/1‑126 (MQ=38)                                                                                         cATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGa                                                 <  1:731880/126‑1 (MQ=255)                                                                                           tCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTgtggtg                                 >  2:1436123/1‑140 (MQ=37)                                                                                           tCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTgtggtg                                 >  2:635851/1‑140 (MQ=37)                                                                                               gACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCa                                         <  1:1001572/128‑1 (MQ=38)                                                                                               gACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCa                                         >  2:1001572/1‑128 (MQ=38)                                                                                                aCGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTccc                            <  1:395484/140‑1 (MQ=37)                                                                                                  gATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCccgc                          <  2:1009796/140‑1 (MQ=37)                                                                                                   aTTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGGGGCGAAGACGGTGGCAGTGTGGTGTTCccgcc                         <  2:64670/140‑1 (MQ=255)                                                                                                   aTTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCccgcc                         >  2:1452656/1‑140 (MQ=37)                                                                                                        tCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGc                    <  1:1452656/140‑1 (MQ=37)                                                                                                        tCAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGc                    <  2:333831/140‑1 (MQ=37)                                                                                                        ccAGCCGGGGCGCTTCACCCCACGCTTCGGGGCTGAAACTGCCAGGGGGGGGCTGGAAAAAACGCAGCGCGGCAGCGTGGCGGATGCCCTCCGGCAGTCCGGGGGGGAAGAGGGTGGCTGTGTGGTGTTCCCGCCGGTGc                    <  2:1030996/139‑1 (MQ=2)                                                                                                         cAGCCGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCt                   <  2:1125048/140‑1 (MQ=37)                                                                                                            ccGGGGAGCTTCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGt                >  2:458245/1‑140 (MQ=255)                                                                                                                      tCACGCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTc      >  1:320994/1‑140 (MQ=37)                                                                                                                          gCCACTCATCAGGGCCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGAcc  >  2:564057/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                              GCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACC  >  NC_000913/3622232‑3622492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑