Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	476,580	T→C	intergenic (+19/‑92)	ffs → / → ybaA	signal recognition particle 4.5S RNA/DUF1428 domain‑containing protein YbaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	476,580	0	T	C	100.0%	329.2 / NA	95	intergenic (+19/‑92)	ffs/ybaA	signal recognition particle 4.5S RNA/DUF1428 domain‑containing protein YbaA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/50);  total (45/50)

TCAATGGGGGCTCTGTTGGTTCTCCCGCAACGCTACTCTGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTTTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGTATGTCTGATGAAGTATGTTGATGGTTTTGTGGTTGCCGTTCCTGCCGATA  >  NC_000913/476443‑476714                                                                                                                                          |                                                                                                                                       tcAATGGGGGCTCTGTTGGTTCTCCCGCAACGCTACTCTGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGtctc                                                                                                                                      >  1:748728/1‑140 (MQ=255) tcAATGGGGGCTCTGTTGGTTCTCCCGCAACGCTACTCTGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGtctc                                                                                                                                      >  2:502700/1‑140 (MQ=255)  cAATGGGGGCTCTGTTGGTTCTCCCGCAACGCTACTCTGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCg                                                                                                                                     >  1:703460/1‑140 (MQ=255)        gggCTCTGTTGGTTCTCCCGCAACGCTACTCTGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCaaaa                                                                                                                               >  2:1091007/1‑140 (MQ=255)         ggCTCTGTTGGTTCTCCCGCAACGCTACTCTGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCaaaaa                                                                                                                              >  2:1393890/1‑140 (MQ=255)           ctctGTTGGTTCTCCCGCAACGCTACTCTGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGTCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAAt                                                                                                                            >  1:977434/1‑140 (MQ=255)           ctctGTTGGTTCTCCCGCAACGCTACTCTGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAAt                                                                                                                            >  1:1349047/1‑140 (MQ=255)           ctctGTTGGTTCTCCCGCAACGCTACTCTGTTTACCAGGTAAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAAt                                                                                                                            >  1:1468332/1‑140 (MQ=255)             ctGTTGGTTCTCCCGCAACGCTACTCTGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATcc                                                                                                                          >  1:1108795/1‑140 (MQ=255)             ctGTTGGTTCTCCCGCAACGCTACTCTGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATcc                                                                                                                          >  1:1230316/1‑140 (MQ=255)             ctGTTGGTTCTCCCGCAACGCTACTCTGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATcc                                                                                                                          >  2:137583/1‑140 (MQ=255)                ttGGTTCTCCCGCAACGCTACTCTGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCaa                                                                                                                       >  2:744185/1‑140 (MQ=255)                ttGGTTCTCCCGCAACGCTACTCTGTTTACCAGGTAAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCaa                                                                                                                       <  2:1468332/140‑1 (MQ=255)                      ctcCCGCAACGCTACTCTGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGc                                                                                                                 >  2:577793/1‑140 (MQ=255)                       tcCCGCAACGCTACTCTGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCt                                                                                                                <  2:548366/140‑1 (MQ=255)                       tcCCGCAACGCTACTCTGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCt                                                                                                                <  1:547235/140‑1 (MQ=255)                        cccGCAACGCTACTCTGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCaa                                                                                                                       <  2:1415507/132‑1 (MQ=255)                        cccGCAACGCTACTCTGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCaa                                                                                                                       >  1:1415507/1‑132 (MQ=255)                        cccGCAACGCTACTCTGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTa                                                                                                               >  2:1256568/1‑140 (MQ=255)                        cccGCAACGCTACTCTGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTa                                                                                                               <  1:341600/140‑1 (MQ=255)                        cccGCAACGCTACTCTGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTa                                                                                                               >  1:465823/1‑140 (MQ=255)                         ccGCAACGCTACTCTGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTaa                                                                                                              >  2:396228/1‑140 (MQ=255)                         ccGCAACGCTACTCTGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTaa                                                                                                              <  2:1488767/140‑1 (MQ=255)                            cAACGCTACTCTGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACt                                                                                                           <  2:703460/140‑1 (MQ=255)                            cAACGCTACTCTGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACt                                                                                                           <  1:396228/140‑1 (MQ=255)                            cAACGCTACTCTGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACt                                                                                                           <  1:402471/140‑1 (MQ=255)                              aCGCTACTCTGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACttt                                                                                                         >  2:443279/1‑140 (MQ=255)                                 cTACTCTGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAAc                                                                                                      <  2:1419152/140‑1 (MQ=255)                                  tACTCTGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAAcc                                                                                                     <  2:748728/140‑1 (MQ=255)                                   aCTCTGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACca                                                                                                    >  1:1270783/1‑140 (MQ=255)                                       tGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAAc                                                                                                >  2:485578/1‑140 (MQ=255)                                         tttACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCATAAAATCCCAACATGGCTAAACTTTAACCACAACTg                                                                                              >  2:1055969/1‑140 (MQ=255)                                         tttACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTGAACTTTAACCACAACTg                                                                                              >  2:150489/1‑140 (MQ=255)                                         tttACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTg                                                                                              >  1:1477575/1‑140 (MQ=255)                                           tACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGAc                                                                                            >  2:585671/1‑140 (MQ=255)                                              caggtcaggtCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTc                                                                                         >  1:1309483/1‑140 (MQ=255)                                               aggtcaggtcCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCg                                                                                        <  2:1143869/140‑1 (MQ=255)                                                ggtcaggtcCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGc                                                                                       <  1:1091007/140‑1 (MQ=255)                                                   caggtcCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAg                                                                                    <  1:443279/140‑1 (MQ=255)                                                   caggtcCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAg                                                                                    >  2:293402/1‑140 (MQ=255)                                                     ggtcCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGaa                                                                                  <  1:502700/140‑1 (MQ=255)                                                      gtcCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAAt                                                                                 >  1:403906/1‑140 (MQ=255)                                                          ggaaggaaGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTc                                                                             <  1:1393890/140‑1 (MQ=255)                                                           gaaggaagCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCt                                                                            >  1:597539/1‑140 (MQ=255)                                                             aggaagCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTgg                                                                          <  1:744185/140‑1 (MQ=255)                                                               gaagCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCt                                                                        <  1:1256568/140‑1 (MQ=255)                                                               gaagCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCt                                                                        <  2:977434/140‑1 (MQ=255)                                                                aagCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTg                                                                       >  1:787675/1‑140 (MQ=255)                                                                aagCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTg                                                                       >  2:1277900/1‑140 (MQ=255)                                                                 agcagcCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTg                                                                               >  1:399065/1‑131 (MQ=255)                                                                 agcagcCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTg                                                                               <  2:399065/131‑1 (MQ=255)                                                                  gcagcCAAGGCAGATGACGCGTGTGCCGGGATGTAGCCGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTgcg                                                                     <  1:623335/140‑1 (MQ=255)                                                                      ccAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGt                                                                 <  2:911743/140‑1 (MQ=255)                                                                      ccAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGt                                                                 <  1:1277900/140‑1 (MQ=255)                                                                       cAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTa                                                                <  1:628592/140‑1 (MQ=255)                                                                       cAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTa                                                                <  2:1230316/140‑1 (MQ=255)                                                                         aGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACg                                                              <  1:1390682/140‑1 (MQ=255)                                                                         aGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACg                                                              <  2:403906/140‑1 (MQ=255)                                                                         aGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTC‑AAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGc                                                             >  2:104083/1‑140 (MQ=255)                                                                          ggCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGc                                                             >  1:1413773/1‑140 (MQ=255)                                                                             aGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTc                                                          <  2:465823/140‑1 (MQ=255)                                                                              gATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCg                                                         <  2:1309483/140‑1 (MQ=255)                                                                                tGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTgcgc                                                                    <  2:121508/127‑1 (MQ=255)                                                                                tGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTgcgc                                                                    >  1:121508/1‑127 (MQ=255)                                                                                tGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTa                                                                >  1:552378/1‑131 (MQ=255)                                                                                tGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTa                                                                <  2:552378/131‑1 (MQ=255)                                                                                tGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGa                                                       >  1:152010/1‑140 (MQ=255)                                                                                tGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGa                                                       <  1:528832/140‑1 (MQ=255)                                                                                tGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGa                                                       >  1:134857/1‑140 (MQ=255)                                                                                   cgcgTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGt                                                    <  2:134857/140‑1 (MQ=255)                                                                                   cgcgTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGt                                                    <  1:868602/140‑1 (MQ=255)                                                                                    gcgTGTGCCGGGGTGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGTa                                                   >  2:1519902/1‑140 (MQ=255)                                                                                     cgTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGTAt                                                  <  1:1055969/140‑1 (MQ=255)                                                                                     cgTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGTAt                                                  <  2:597539/140‑1 (MQ=255)                                                                                     cgTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGTAt                                                  <  2:1413773/140‑1 (MQ=255)                                                                                     cgTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGTAt                                                  <  1:585671/140‑1 (MQ=255)                                                                                     cgTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGTAt                                                  >  1:35130/1‑140 (MQ=255)                                                                                          gCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGTATGTCtg                                             <  2:1477575/140‑1 (MQ=255)                                                                                          gCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGTATGTCtg                                             <  2:311780/140‑1 (MQ=255)                                                                                           ccGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTGAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGTATGTCtga                                            <  1:150489/140‑1 (MQ=255)                                                                                            cGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGTATGTCtgat                                           <  2:787675/140‑1 (MQ=255)                                                                                              ggATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGTATGTCtgatga                                         <  2:599756/140‑1 (MQ=255)                                                                                                      cTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGTATGTCTGATGAAGTATGtt                                 >  1:446999/1‑140 (MQ=255)                                                                                                        ggCAGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGTATGTCTGATGAAGTATGTTGa                               <  2:1108795/140‑1 (MQ=255)                                                                                                           aGGGCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGTATGTCTGATGAAGTATGTTGATgg                            >  1:314220/1‑140 (MQ=255)                                                                                                             ggCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGTATGTCTGATGAAGTATGTTGATGGtt                          <  1:1519902/140‑1 (MQ=255)                                                                                                             ggCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGTATGTCTGATGAAGTATGTTGATGGtt                          >  1:282066/1‑140 (MQ=255)                                                                                                             ggCCCCCACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGTATGTCTGATGAAGTATGTTGATGGtt                          >  2:13136/1‑140 (MQ=255)                                                                                                                ccccACCCATTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGTATGTCTGATGAAGTATGTTGATGGTTTtg                       <  1:485578/140‑1 (MQ=255)                                                                                                                 cccacccaTTTCTGCCTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGTATGTCTGATGAAGTATGTTGATGGTTTtgt                      <  1:1494749/140‑1 (MQ=255)                                                                                                                               ccTCCCACCGTCTCGTC‑AAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGTATGTCTGATGAAGTATGTTGATGGTTTTGTGGTTGCCGTTCCTGc       <  1:104083/140‑1 (MQ=255)                                                                                                                                cTCCCACCGTCTCGTCAAAAAATCCCAACGTGGCTAAACTTTAACCACAACTGACGTCGAAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGTATGTCTGATGAAGTATGTTGATGGTTTTGTGGTTGCCGTTCCTGc       <  1:137583/140‑1 (MQ=255)                                                                                                                                cTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGTATGTCTGATGAAGTATGTTGATGGTTTTGTGGTTGCCGTTTCTGc       <  2:35130/140‑1 (MQ=255)                                                                                                                                cTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGTATGTCTGATGAAGTATGTTGATGGTTTTGTGGTTGCCGTTCCTGc       >  1:1059924/1‑140 (MQ=255)                                                                                                                                cTCCCACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGTATGTCTGATGAAGTATGTTGATGGTTTTGTGGTTGCCGTTCCTGc       >  2:781486/1‑140 (MQ=255)                                                                                                                                    cACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGTATGTCTGATGAAGTATGTTGATGGTTTTGTGGTTGCCGTTCCTGCCGAt   >  1:49020/1‑140 (MQ=255)                                                                                                                                    cACCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGTATGTCTGATGAAGTATGTTGATGGTTTTGTGGTTGCCGTTCCTGCCGAt   >  1:602804/1‑140 (MQ=255)                                                                                                                                     aCCGTCTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGTATGTCTGATGAAGTATGTTGATGGTTTTGTGGTTGCCGTTCCTGCCGATa  <  2:152010/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       TCAATGGGGGCTCTGTTGGTTCTCCCGCAACGCTACTCTGTTTACCAGGTCAGGTCCGGAAGGAAGCAGCCAAGGCAGATGACGCGTGTGCCGGGATGTAGCTGGCAGGGCCCCCACCCATTTCTGCCTCCCACCGTTTCGTCAAAAAATCCCAACATGGCTAAACTTTAACCACAACTGACGTCGCAAGAATTGTCTGGCTGCGCAGTACGCTTCGGAGGTATGTCTGATGAAGTATGTTGATGGTTTTGTGGTTGCCGTTCCTGCCGATA  >  NC_000913/476443‑476714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑