Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,711,960	A→G	G16G (GGT→GGC)	yhjX ←	putative pyruvate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,711,960	0	A	G	97.0%	220.5 / ‑3.4	67	G16G (GGT→GGC)	yhjX	putative pyruvate transporter
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (29/36);  total (30/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAATTGCCAGCCCCAGACTTAACAAGCCGAAAGAGAAAGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTGCACTTTATGCCTGGGGAAAAACG  >  NC_000913/3711823‑3712088                                                                                                                                          |                                                                                                                                 gAAATTGCCAGCCCCAGACTTAACAAGCCGAAAGAGAAAGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGcc                                                                                                                                >  2:1407459/1‑140 (MQ=255)    aTTGCCAGCCCCAGACTTAACAAGCCGAAAGAGAAAGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGAt                                                                                                                             <  1:903147/140‑1 (MQ=255)    aTTGCCAGCCCCAGACTTAACAAGCCGAAAGAGAAAGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGAt                                                                                                                             <  2:133315/140‑1 (MQ=255)    aTTGCCAGCCCCAGACTTAACAAGCCGAAAGAGAAAGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGAGTAATGATAGTGCCGAt                                                                                                                             <  2:1486541/140‑1 (MQ=255)      tGCCAGCCCCAGACTTAACAAGCCGAAAGAGAAAGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGa                                                                                                                           >  2:1025742/1‑140 (MQ=255)        ccAGCCCCAGACTTAACAAGCAGAAAGAGAAAGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAgt                                                                                                                         >  2:1086804/1‑140 (MQ=255)          aGCCCCAGACTTAACAAGCCGAAAGAGAAAGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAgtgt                                                                                                                       <  1:703358/140‑1 (MQ=255)            ccccAGACTTAACAAGCCGAAAGAGAAAGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTca                                                                                                                     >  2:1517954/1‑140 (MQ=255)             cccAGACTTAACAAGCCGAAAGAGAAAGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTcag                                                                                                                    <  1:51684/140‑1 (MQ=255)                     tAACAAGCCGAAAGAGAAAGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCggg                                                                                                            <  1:1025742/140‑1 (MQ=255)                      aaCAAGCCGAAAGAGAAAGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGAGGAGTGTCAGCCAGCgggc                                                                                                           >  2:1190337/1‑139 (MQ=255)                         aaGCCGAAAGAGAAAGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACg                                                                                                        <  2:114741/140‑1 (MQ=255)                          aGCCGAAAGAGAAAGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTcag                                                                                                                    >  1:1200132/1‑127 (MQ=255)                          aGCCGAAAGAGAAAGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTcag                                                                                                                    <  2:1200132/127‑1 (MQ=255)                            ccGAAAGAGAAAGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTa                                                                                                          <  2:539193/135‑1 (MQ=255)                            ccGAAAGAGAAAGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTa                                                                                                          >  1:539193/1‑135 (MQ=255)                                    gaAAGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTg                                                                                             <  2:56048/140‑1 (MQ=255)                                     aaaGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGa                                                                                            <  2:277149/140‑1 (MQ=255)                                      aaGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGaa                                                                                           <  1:481130/140‑1 (MQ=255)                                      aaGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGaa                                                                                           >  1:540172/1‑140 (MQ=255)                                       aGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAg                                                                                          >  2:524570/1‑140 (MQ=255)                                         cGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGgt                                                                                        >  1:968887/1‑140 (MQ=255)                                            ccTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGaa                                                                                           <  1:1096119/134‑1 (MQ=255)                                            ccTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGaa                                                                                           >  2:1096119/1‑134 (MQ=255)                                             cTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGaa                                                                                           <  1:1531520/133‑1 (MQ=255)                                             cTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGaa                                                                                           >  2:1531520/1‑133 (MQ=255)                                               ggCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTg                                                                                             <  1:573969/129‑1 (MQ=255)                                               ggCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTg                                                                                             >  2:573969/1‑129 (MQ=255)                                                gCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATgg                                                                                 <  1:1407459/140‑1 (MQ=255)                                                 cTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGc                                                                                <  2:968887/140‑1 (MQ=255)                                                   tACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAg                                                                              <  2:1509834/140‑1 (MQ=255)                                                   tACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAg                                                                              <  1:1517954/140‑1 (MQ=255)                                                      cGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTAt                                                                           >  1:1488221/1‑140 (MQ=255)                                                          gcATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCAt                                                                                   <  1:1020371/128‑1 (MQ=255)                                                          gcATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCAt                                                                                   >  2:1020371/1‑128 (MQ=255)                                                          gcATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCt                                                                       >  2:665683/1‑140 (MQ=255)                                                                  cTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGtaat                                                               >  1:727163/1‑140 (MQ=255)                                                                           aaGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGaa                                                      >  2:1139098/1‑140 (MQ=255)                                                                                      tAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGcc                                           >  1:1222578/1‑140 (MQ=255)                                                                                         aCAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAGAAGGAAAAATCTCTGCCGAc                                        <  2:1488221/140‑1 (MQ=255)                                                                                         aCAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGAc                                        <  2:121305/140‑1 (MQ=255)                                                                                            ggCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAAc                                     <  1:1086804/140‑1 (MQ=255)                                                                                             gCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAAcc                                    >  1:1513291/1‑140 (MQ=255)                                                                                               tCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGGAAAATCTCTGCCGACAACCAt                                  >  2:1395601/1‑140 (MQ=255)                                                                                               tCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCAt                                  >  2:1039110/1‑140 (MQ=255)                                                                                                ccAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATa                                 >  1:868766/1‑140 (MQ=255)                                                                                                ccAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATa                                 <  2:320228/140‑1 (MQ=255)                                                                                                   ggTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACg                              <  1:665683/140‑1 (MQ=255)                                                                                                   ggTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACg                              <  2:868766/140‑1 (MQ=255)                                                                                                    gTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGAc                                        >  2:1166460/1‑129 (MQ=255)                                                                                                    gTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGAc                                        <  1:1166460/129‑1 (MQ=255)                                                                                                       taAACCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGt                          <  1:1039110/140‑1 (MQ=255)                                                                                                        aaaCCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTg                         <  2:1513291/140‑1 (MQ=255)                                                                                                        aaaCCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTg                         <  2:448789/140‑1 (MQ=255)                                                                                                        aaaCCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTg                         <  1:1139098/140‑1 (MQ=255)                                                                                                        aaaCCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTg                         <  2:727163/140‑1 (MQ=255)                                                                                                          aCCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAG‑AATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTg                         <  2:1098580/137‑1 (MQ=255)                                                                                                          aCCGACCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAG‑AATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTg                         >  1:1098580/1‑137 (MQ=255)                                                                                                              aCCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTg                         >  2:164403/1‑134 (MQ=255)                                                                                                              aCCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTg                         <  1:164403/134‑1 (MQ=255)                                                                                                              aCCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTGCACttt                   >  2:488379/1‑140 (MQ=255)                                                                                                              aCCCCAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTGCACttt                   <  2:809504/140‑1 (MQ=255)                                                                                                               ccccAGCGCAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTGCACTTTa                  >  2:1291671/1‑140 (MQ=255)                                                                                                                    gcgcAAACTGGGTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTGCACTTTATGCCt             >  2:293290/1‑140 (MQ=255)                                                                                                                             gggTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTGCACTTTATGCCTGGGGaaaaa    <  1:524570/140‑1 (MQ=255)                                                                                                                               gTAATGATAGTGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTGCACTTTATGCCTGGGGAAAAACg  <  2:144538/140‑1 (MQ=255)                                                                                                                               gTAATGATAGCGCCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTGCACTTTATGCCTGGGGAAAAACg  >  2:1423304/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                 GAAATTGCCAGCCCCAGACTTAACAAGCCGAAAGAGAAAGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTGCACTTTATGCCTGGGGAAAAACG  >  NC_000913/3711823‑3712088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑