Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,904,307	T→C	S431G (AGC→GGC)	bglF ←	beta‑glucoside specific PTS enzyme II/BglG kinase/BglG phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,904,307	0	T	C	100.0%	264.6 / NA	76	S431G (AGC→GGC)	bglF	beta‑glucoside specific PTS enzyme II/BglG kinase/BglG phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/35);  total (41/35)

GCTCAGGTGGTGTAATAACCTCTGGTGTTTTCTCTTGCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGC  >  NC_000913/3904172‑3904442                                                                                                                                         |                                                                                                                                        gCTCAGGTGGTGTAATAACCTCTGGTGTTTTCTCTTGCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGcc                                                                                                                                     >  1:1239656/1‑140 (MQ=255)          gtgtAATAACCTCTGGTGTTTTCTCTTGCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTg                                                                                                                            >  1:1432973/1‑140 (MQ=255)            gtAATAACCTCTGGTGTTTTCTCTTGCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGAACCGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGaa                                                                                                                          >  2:268139/1‑140 (MQ=255)                 aaCCTCTGGTGTTTTCTCTTGCGGGGCCCCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGaa                                                                                                                          <  1:437463/135‑1 (MQ=255)                 aaCCTCTGGTGTTTTCTCTTGCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGaa                                                                                                                          >  2:437463/1‑135 (MQ=255)                 aaCCTCTGGTGTTTTCTCTTGCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCaa                                                                                                                     >  1:628994/1‑140 (MQ=255)                 aaCCTCTGGTGTTTTCTCTTGCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCaa                                                                                                                     >  2:657345/1‑140 (MQ=255)                    ctctGGTTTTTTCTCTTGCGGGGCCCCCTGCGCGGGCTGACGTTTAGCGGTGATGAAATGAAGCACCCCCGTCCCGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTc                                                                                                                  <  2:82565/140‑1 (MQ=255)                     tctGGTGTTTTCTCTTGCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTcc                                                                                                                 >  1:1020294/1‑140 (MQ=255)                     tctGGTGTTTTCTCTTGCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTcc                                                                                                                 >  1:1390649/1‑140 (MQ=255)                     tctGGTGTTTTCTCTTGCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTcc                                                                                                                 >  2:967587/1‑140 (MQ=255)                       tGGTGTTTTCTCTTGCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCg                                                                                                               >  1:1462654/1‑140 (MQ=255)                       tGGTGTTTTCTCTTGCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCg                                                                                                               >  1:436005/1‑140 (MQ=255)                        cggtttttCTCTGGGGGGGCCCCCTGCGCGGGTTGACGTTTGGGGGTAATAAAATAAAGCATCACCGTACCGAAAAATGCGCAACCGATGG‑AAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGt                                                                                                              <  2:979970/136‑1 (MQ=255)                          ttttttCTCTTGCGGGGCCCCCTGCGCTGGCTGACGTTTAGCGGTGATAAAATGAAGCATCCCCGTCCCGAAAAACGCGAAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTg                                                                                                            <  2:88596/138‑1 (MQ=255)                              ttCTCTTGCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACgg                                                                                                        >  1:545030/1‑140 (MQ=255)                               tctcTTGGGGGGCCCCCGGCGCGGGTTGACGTTTGGCGGTGAAAAAATGAAGCATCACGGTACCGAAAAAGGCGCACCCGAGGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACggg                                                                                                       <  2:628994/140‑1 (MQ=255)                               tctcTGGGGGGGCCCCCTGCGCTGGTTGACGTTTGGCGGTGATAAAATGAAGCATCCCCGTACCGACAAATGCGCAACCGATGG‑TAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACggg                                                                                                       <  2:1241481/140‑1 (MQ=255)                                ctctTGCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGa                                                                                                      <  2:436005/140‑1 (MQ=255)                                ccctggCGGGGCCCCCTGCGCGGGGTGACGTTTGGCGGTAATGAAATAAAGCATCCCCGTCCCGACAAATGCGCAACCGAGGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGa                                                                                                      <  2:1390649/135‑1 (MQ=255)                                 tctTGCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGAt                                                                                                     >  2:888721/1‑140 (MQ=255)                                      cGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATggctg                                                                                                >  1:979970/1‑137 (MQ=255)                                                   gctggctgACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGaa                                                                                   >  1:904624/1‑140 (MQ=255)                                                            cGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTgg                                                                          >  2:1338147/1‑140 (MQ=255)                                                            cGTTTAGCGGTGATAAAATGAAGCATCACCGTACCGAAAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTgg                                                                          <  1:183388/140‑1 (MQ=255)                                                                 aGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAAc                                                                     >  2:1416714/1‑140 (MQ=255)                                                                    ggTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCaa                                                                  <  2:1239656/140‑1 (MQ=255)                                                                        atgaaatgaaGCATCACCGTACCGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGa                                                              >  2:976869/1‑140 (MQ=255)                                                                          gaaatgaaGCATCACCGTACCGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGt                                                            <  1:657345/140‑1 (MQ=255)                                                                          aaaatgaaGCATCACCGTCCCGACAAATGCGCAACCGATGG‑CAATAACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGt                                                            <  1:967661/139‑1 (MQ=255)                                                                          aaaaggaaGCACCCCCGTCCCGAAAAATGCGAACCCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGt                                                            <  2:1459018/139‑1 (MQ=255)                                                                                aaGCATCACCGTACCGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACtt                                                      >  2:961503/1‑140 (MQ=255)                                                                                aaGCATCACCGTACCGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACtt                                                      <  2:999661/140‑1 (MQ=255)                                                                                aaGCATCACCGTACCGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACtt                                                      >  1:999661/1‑140 (MQ=255)                                                                                aaGCATCACCGTACCGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACtt                                                      >  2:1154366/1‑140 (MQ=255)                                                                                      cACCGTACCGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGttt                                                >  1:285345/1‑140 (MQ=255)                                                                                           tACCGACAAATGCGCAACCGATGG‑AAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGcgcg                                           <  1:806039/140‑1 (MQ=255)                                                                                             cggACAACTGCCC‑ACCGATGGCCAGTGACCCCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTa                                         <  2:1432973/138‑1 (MQ=255)                                                                                              cGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGTGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAg                                        <  1:967587/140‑1 (MQ=255)                                                                                               gACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGc                                       >  2:884076/1‑140 (MQ=255)                                                                                               gACAAAGGCACAACGGAGGG‑CACTGCCGCCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGc                                       <  2:193033/140‑1 (MQ=255)                                                                                                aCAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGcc                                      >  1:539636/1‑140 (MQ=255)                                                                                                aCAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGcc                                      >  1:1507626/1‑140 (MQ=255)                                                                                                aCAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGcc                                      >  2:1119497/1‑140 (MQ=255)                                                                                                aCAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGcc                                      >  1:1349457/1‑140 (MQ=255)                                                                                                  aaaTGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCaa                                    >  1:128337/1‑140 (MQ=255)                                                                                                   aaTGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCaat                                   >  1:1118509/1‑140 (MQ=255)                                                                                                   aaTGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCaat                                   >  2:1235185/1‑140 (MQ=255)                                                                                                   aaTGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCaat                                   >  1:193033/1‑140 (MQ=255)                                                                                                     tGCGCAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCaataa                                 >  1:908009/1‑140 (MQ=255)                                                                                                       cgcAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAAtg                               <  1:1119497/140‑1 (MQ=255)                                                                                                       cgcAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAAtg                               >  1:1466537/1‑140 (MQ=255)                                                                                                       cgcAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAAtg                               >  1:1486622/1‑140 (MQ=255)                                                                                                       cgcAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAAtg                               >  2:1436237/1‑140 (MQ=255)                                                                                                       cgcAACCGATGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAAtg                               >  1:789533/1‑140 (MQ=255)                                                                                                               aTGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGcccc                       >  2:1256242/1‑140 (MQ=255)                                                                                                               aTGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGcccc                       >  1:562684/1‑140 (MQ=255)                                                                                                                tGG‑CAATGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGccccc                      <  1:1235185/140‑1 (MQ=255)                                                                                                                      aagACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGc                 <  2:28228/138‑1 (MQ=255)                                                                                                                      aTGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAGAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGc                 <  2:789533/140‑1 (MQ=255)                                                                                                                      aTGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGc                 <  2:1462654/140‑1 (MQ=255)                                                                                                                      aTGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGc                 <  1:1436237/140‑1 (MQ=255)                                                                                                                       tGACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGcc                >  2:667502/1‑140 (MQ=255)                                                                                                                        gACACCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGccc               <  1:667502/140‑1 (MQ=255)                                                                                                                         acacCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATCCTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGcccc              <  1:1338147/140‑1 (MQ=255)                                                                                                                          cacCGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCa             <  1:961503/140‑1 (MQ=255)                                                                                                                             cGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGc                 >  2:393773/1‑133 (MQ=255)                                                                                                                             cGCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGc                 <  1:393773/133‑1 (MQ=255)                                                                                                                              gCCAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGa         >  2:578559/1‑140 (MQ=255)                                                                                                                                cAATAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATa       <  2:545030/140‑1 (MQ=255)                                                                                                                                  aTAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATa       >  2:804682/1‑138 (MQ=255)                                                                                                                                  aTAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATa       <  1:804682/138‑1 (MQ=255)                                                                                                                                  aTAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACa     <  1:1136997/140‑1 (MQ=255)                                                                                                                                  aTAACGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACa     <  2:128337/140‑1 (MQ=255)                                                                                                                                     aCGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGc  <  1:976869/140‑1 (MQ=255)                                                                                                                                     aCGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGc  <  1:268139/140‑1 (MQ=255)                                                                                                                                     aCGCCGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGc  <  1:1154366/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        GCTCAGGTGGTGTAATAACCTCTGGTGTTTTCTCTTGCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGG‑CAATGACACCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGC  >  NC_000913/3904172‑3904442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑