Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	486,373	T→C	C205R (TGC→CGC)	acrR →	DNA‑binding transcriptional repressor AcrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	486,373	0	T	C	100.0%	302.9 / NA	91	C205R (TGC→CGC)	acrR	DNA‑binding transcriptional repressor AcrR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (54/37);  total (54/37)

GCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGACTTTTTCAGGTCGT  >  NC_000913/486240‑486507                                                                                                                                      |                                                                                                                                       gcgTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCa                                                                                                                                  >  1:903908/1‑140 (MQ=255) gcgTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCACCa                                                                                                                                  <  1:241754/140‑1 (MQ=255)  cgTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCa                                                                                                                                  <  1:736009/139‑1 (MQ=255)  cgTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCa                                                                                                                                  >  2:736009/1‑139 (MQ=255)  cgTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCAc                                                                                                                                 <  1:523219/140‑1 (MQ=255)  cgTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCAc                                                                                                                                 <  2:608388/140‑1 (MQ=255)   gTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACg                                                                                                                                <  1:497712/140‑1 (MQ=255)   gTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACg                                                                                                                                <  1:405411/140‑1 (MQ=255)   gTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACg                                                                                                                                <  1:6193/140‑1 (MQ=255)   gTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACg                                                                                                                                <  2:903908/140‑1 (MQ=255)   gTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACg                                                                                                                                <  1:1240155/140‑1 (MQ=255)     cgcgCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCt                                                                                                                              <  2:1047866/140‑1 (MQ=255)            caATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAAt                                                                                                                       <  1:880816/140‑1 (MQ=255)             aaTTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATc                                                                                                                      <  2:580832/140‑1 (MQ=255)                 attatGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGc                                                                                                                  >  2:524788/1‑140 (MQ=255)                 attatGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGc                                                                                                                  <  2:15766/140‑1 (MQ=255)                 attatGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGc                                                                                                                  <  1:958839/140‑1 (MQ=255)                    atGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCAc                                                                                                               >  1:1197012/1‑140 (MQ=255)                      gcgcGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTa                                                                                                             >  1:1233751/1‑140 (MQ=255)                      gcgcGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTa                                                                                                             <  2:206046/140‑1 (MQ=255)                       cgcgGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGTCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTaa                                                                                                            >  1:202809/1‑140 (MQ=255)                        gcgGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAAc                                                                                                           >  2:481954/1‑140 (MQ=255)                          ggCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGa                                                                                                         >  2:754552/1‑140 (MQ=255)                           gCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGaa                                                                                                        <  2:1193036/140‑1 (MQ=255)                            cTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAAt                                                                                                       <  1:551930/140‑1 (MQ=255)                                 tttCCGGCCTGATGGAAAACTGGCTCTTTGTCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAAccc                                                                                                  <  2:202809/140‑1 (MQ=255)                                      ggCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAAt                                                                                             >  1:1438138/1‑140 (MQ=255)                                          tGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGa                                                                                         >  2:1392286/1‑140 (MQ=255)                                            aTGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACt                                                                                       >  2:773555/1‑140 (MQ=255)                                                 aaaCTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAgg                                                                                  >  2:509525/1‑140 (MQ=255)                                                    cTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGAtt                                                                               >  2:1422701/1‑140 (MQ=255)                                                      ggCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGAt                                                                                >  1:592449/1‑137 (MQ=255)                                                      ggCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGAt                                                                                <  2:592449/137‑1 (MQ=255)                                                       gCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGAttttt                                                                            >  2:717685/1‑140 (MQ=255)                                                       gCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGAttttt                                                                            >  1:807914/1‑140 (MQ=255)                                                            ttGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTgg                                                                       >  2:1012720/1‑140 (MQ=255)                                                                      aTCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTtcgt                                                             <  1:524788/140‑1 (MQ=255)                                                                      aTCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTtcgt                                                             <  1:717685/140‑1 (MQ=255)                                                                       tCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTtcgtc                                                            >  2:139573/1‑140 (MQ=255)                                                                       tCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTtcgtc                                                            >  2:1092994/1‑140 (MQ=255)                                                                         ttttGATCTAAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTcgtcgt                                                          <  2:474743/140‑1 (MQ=255)                                                                              aTCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTa                                                     <  1:1012720/140‑1 (MQ=255)                                                                               tCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTAt                                                    >  1:515118/1‑140 (MQ=255)                                                                                 ttAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTc                                                  >  2:177760/1‑140 (MQ=255)                                                                                 ttAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTc                                                  >  1:1096883/1‑140 (MQ=255)                                                                                 ttAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTc                                                  >  1:897519/1‑140 (MQ=255)                                                                                   aaaaaaGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTg                                                >  1:1269373/1‑140 (MQ=255)                                                                                   aaaaaaGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTg                                                >  2:361883/1‑140 (MQ=255)                                                                                          aagCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACt                                         >  1:1375234/1‑140 (MQ=255)                                                                                          aagCCCCAGATTACGTTGCCATCTTACGGGAGAGGTATCTCCTGCGCCCCTCATTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACt                                         <  1:1392286/140‑1 (MQ=255)                                                                                           agCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTg                                        <  2:807914/140‑1 (MQ=255)                                                                                             cccGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGcg                                      >  2:377809/1‑140 (MQ=255)                                                                                             cccGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGcg                                      <  1:361883/140‑1 (MQ=255)                                                                                             cccGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTAGACATTTTCGTCGTTGCTATTCTGGTTCACt                                         <  2:1334288/137‑1 (MQ=255)                                                                                             cccGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTAGACATTTTCGTCGTTGCTATTCTGGTTCACt                                         >  1:1334288/1‑137 (MQ=255)                                                                                              ccGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGcgt                                     <  1:772955/140‑1 (MQ=255)                                                                                               cgcgATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCGCt                                         <  1:501175/135‑1 (MQ=255)                                                                                               cgcgATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACt                                         >  2:501175/1‑135 (MQ=255)                                                                                                 cgATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTAtt                                                   <  1:14054/123‑1 (MQ=255)                                                                                                 cgATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTAtt                                                   >  2:14054/1‑123 (MQ=255)                                                                                                 cgATTACGTTGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGcgtcgt                                  >  1:1315238/1‑140 (MQ=255)                                                                                                         ttGCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCt                          >  2:897789/1‑140 (MQ=255)                                                                                                           gCCATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTg                        >  1:1391781/1‑140 (MQ=255)                                                                                                             cATCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCg                      >  1:228015/1‑140 (MQ=255)                                                                                                              aTCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCgg                     >  1:1350471/1‑140 (MQ=255)                                                                                                              aTCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCgg                     >  2:476513/1‑140 (MQ=255)                                                                                                              aTCTTACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCgg                     >  2:998975/1‑140 (MQ=255)                                                                                                                 ttACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGttt                  >  1:1153079/1‑140 (MQ=255)                                                                                                                 ttACCGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGttt                  >  2:665691/1‑140 (MQ=255)                                                                                                                  tACTGGAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTg                 <  2:1233751/140‑1 (MQ=255)                                                                                                                   aCTGGAGATGTATCCCCTGCCCCCCACGTTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGa                <  2:1441791/140‑1 (MQ=255)                                                                                                                      ggAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGACtt             <  1:998975/140‑1 (MQ=255)                                                                                                                      ggAGATGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGACtt             >  2:1349809/1‑140 (MQ=255)                                                                                                                        agaTGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGACtttt           <  2:897519/140‑1 (MQ=255)                                                                                                                         gaTGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGACttttt          >  1:718385/1‑140 (MQ=255)                                                                                                                         gaTGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGACttttt          >  1:114569/1‑140 (MQ=255)                                                                                                                         gaTGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGACttttt          >  2:744894/1‑140 (MQ=255)                                                                                                                          aTGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGa                <  2:393764/133‑1 (MQ=255)                                                                                                                          aTGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGa                <  2:96560/133‑1 (MQ=255)                                                                                                                          aTGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGa                >  1:393764/1‑133 (MQ=255)                                                                                                                          aTGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGa                >  1:96560/1‑133 (MQ=255)                                                                                                                          aTGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGa                <  2:870575/133‑1 (MQ=255)                                                                                                                          aTGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGa                >  1:870575/1‑133 (MQ=255)                                                                                                                          aTGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGACTTTTTc         >  1:957820/1‑140 (MQ=255)                                                                                                                           tGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGACTTTTTCa        <  2:1350471/140‑1 (MQ=255)                                                                                                                           tGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGACTTTTTCa        <  1:509525/140‑1 (MQ=255)                                                                                                                           tGTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGACTTTTTCa        >  1:814384/1‑140 (MQ=255)                                                                                                                            gTATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGACTTTTTCAg       >  1:608397/1‑140 (MQ=255)                                                                                                                             tATCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGACTTTTTCAgg      <  1:1349809/140‑1 (MQ=255)                                                                                                                              aTCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGACTTTTTCAGGt     <  2:1269373/140‑1 (MQ=255)                                                                                                                              aTCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGACTTTTTCAGGt     >  2:5737/1‑140 (MQ=255)                                                                                                                              aTCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGACTTTTTCAGGt     >  1:347121/1‑140 (MQ=255)                                                                                                                              aTCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGACTTTTTCAGGt     >  1:1121406/1‑140 (MQ=255)                                                                                                                              aTCTCCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGACTTTTTCAGGt     <  2:957820/140‑1 (MQ=255)                                                                                                                                ctcCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGACTTTTTCAGGTCg   <  1:897789/140‑1 (MQ=255)                                                                                                                                 tcCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGACTTTTTCAGGTCGt  >  2:12233/1‑140 (MQ=255)                                                                                                                                 tcCTGCGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGACTTTTTCAGGTCGt  >  1:619173/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                       GCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGACTTTTTCAGGTCGT  >  NC_000913/486240‑486507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑