Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,142,585	T→C	V19A (GTT→GCT)	frwC →	putative PTS enzyme IIC component FrwC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,142,585	0	T	C	100.0%	262.7 / NA	75	V19A (GTT→GCT)	frwC	putative PTS enzyme IIC component FrwC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/33);  total (42/33)

TGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGC  >  NC_000913/4142450‑4142721                                                                                                                                        |                                                                                                                                         tGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTca                                                                                                                                      >  2:900389/1‑140 (MQ=255)   tttGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTcaca                                                                                                                                    >  1:402350/1‑140 (MQ=255)   tttGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTcaca                                                                                                                                    >  2:1010967/1‑140 (MQ=255)   tttGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTcaca                                                                                                                                    <  2:872551/140‑1 (MQ=255)   tttGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTcaca                                                                                                                                    >  2:410629/1‑140 (MQ=255)   tttGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTcaca                                                                                                                                    >  1:610671/1‑140 (MQ=255)     tGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTcacaca                                                                                                                                  >  1:321868/1‑140 (MQ=255)     tGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTcac                                                                                                                                     >  1:878496/1‑137 (MQ=255)     tGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTcac                                                                                                                                     <  2:878496/137‑1 (MQ=255)        tCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGa                                                                                                                               >  1:725154/1‑140 (MQ=255)         ccGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGAt                                                                                                                              <  2:1516214/140‑1 (MQ=255)            ccTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTcc                                                                                                                           <  1:1028619/140‑1 (MQ=255)              tGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCt                                                                                                                         <  1:410629/140‑1 (MQ=255)                cGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTc                                                                                                                       <  2:284737/140‑1 (MQ=255)                 gATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGTTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATTACGGGCGCTTCACACATGATTCCCTTCg                                                                                                                      <  2:1334058/140‑1 (MQ=255)                      ccTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTa                                                                                                                 >  2:529431/1‑140 (MQ=255)                               tcctccATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGt                                                                                                        >  2:789006/1‑140 (MQ=255)                                         aaaaTATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTgg                                                                                              <  2:610671/140‑1 (MQ=255)                                             tatGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGt                                                                                          <  2:1438845/140‑1 (MQ=255)                                                  aCATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCg                                                                                     <  1:85375/140‑1 (MQ=255)                                                    aTGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCg                                                                                     <  1:1023288/138‑1 (MQ=255)                                                    aTGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCg                                                                                     >  2:1023288/1‑138 (MQ=255)                                                    aTGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTc                                                                                   >  1:1432484/1‑140 (MQ=255)                                                     tGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCa                                                                                  >  2:857837/1‑140 (MQ=255)                                                      gggCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCAt                                                                                 >  2:878172/1‑140 (MQ=255)                                                       ggCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCAtg                                                                                >  2:27749/1‑140 (MQ=255)                                                           ttCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCAtgttgt                                                                            <  2:1239687/140‑1 (MQ=255)                                                            tCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGttt                                                                                        <  1:839797/127‑1 (MQ=255)                                                            tCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGttt                                                                                        >  2:839797/1‑127 (MQ=255)                                                            tCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTAACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCAtgttg                                                                             >  2:915430/1‑138 (MQ=255)                                                            tCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTAACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCAtgttg                                                                             <  1:915430/138‑1 (MQ=255)                                                                  gCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCaa                                                                     <  2:725154/140‑1 (MQ=255)                                                                       ggAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCAtg                                                                                >  2:1197270/1‑124 (MQ=255)                                                                       ggAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCAtg                                                                                <  1:1197270/124‑1 (MQ=255)                                                                       ggAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGgcg                                                                <  1:789006/140‑1 (MQ=255)                                                                       ggAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGgcg                                                                <  1:900389/140‑1 (MQ=255)                                                                       ggAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGgcg                                                                <  1:529431/140‑1 (MQ=255)                                                                             gcTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTTCGTCCTGTTGTTTGGGAAAAGCGCAGGGc                                                          >  2:388134/1‑140 (MQ=255)                                                                              cTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCa                                                              <  1:1463298/135‑1 (MQ=255)                                                                              cTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCa                                                              >  2:1463298/1‑135 (MQ=255)                                                                              cTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGcc                                                         >  1:568947/1‑140 (MQ=255)                                                                                 cagaatgaGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCGGa                                                      <  1:1421728/138‑1 (MQ=255)                                                                                 atgaatgaGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCGGa                                                      <  1:1010967/140‑1 (MQ=255)                                                                                  tgaatgaGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCGGAt                                                     >  2:931366/1‑140 (MQ=255)                                                                                     atgaGTTGGCGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCGGATGcc                                                  <  2:594610/140‑1 (MQ=255)                                                                                      tgaGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCgg                                                       >  1:343139/1‑134 (MQ=255)                                                                                      tgaGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCgg                                                       <  2:343139/134‑1 (MQ=255)                                                                                        aGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTa                                               >  1:687678/1‑140 (MQ=255)                                                                                        aGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTa                                               >  1:72376/1‑140 (MQ=255)                                                                                          ttGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGc                                             <  1:27749/140‑1 (MQ=255)                                                                                          ttGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGCGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGc                                             <  2:1432484/140‑1 (MQ=255)                                                                                            ggTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCgg                                                       <  1:1440728/128‑1 (MQ=255)                                                                                            ggTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCgg                                                       >  2:1440728/1‑128 (MQ=255)                                                                                               gCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTAGTGTTTTATGGCAAAGGCGCCGGGCCGGATGCCGTAGccccgg                                        >  2:1421728/1‑136 (MQ=255)                                                                                                    tCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAAt                                   >  1:309674/1‑140 (MQ=255)                                                                                                        gAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGa                               >  2:1062010/1‑140 (MQ=255)                                                                                                        gAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGa                               >  2:284797/1‑140 (MQ=255)                                                                                                        gAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGa                               >  1:855950/1‑140 (MQ=255)                                                                                                        gAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGa                               >  1:1200055/1‑140 (MQ=255)                                                                                                        gAAAAATACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGa                               >  2:1320307/1‑140 (MQ=255)                                                                                                          aaaaTACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTTCGTCATGTTGTATGGCAAAAGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGaaa                             >  2:1075785/1‑140 (MQ=255)                                                                                                           aaaTACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGaaaa                            <  2:309674/140‑1 (MQ=255)                                                                                                             aTACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGaaaaaa                          <  1:1062010/140‑1 (MQ=255)                                                                                                             aTACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGaaaaaa                          <  2:855950/140‑1 (MQ=255)                                                                                                             aTACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGaaaaaa                          <  1:388134/140‑1 (MQ=255)                                                                                                              tACCCGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAAc                         >  1:177030/1‑140 (MQ=255)                                                                                                                 ccGTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTTCGTCCTGTTTTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGt                      >  2:891254/1‑140 (MQ=255)                                                                                                                   gTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGttt                    >  1:2528/1‑140 (MQ=255)                                                                                                                   gTCAGCATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGttt                    >  1:1250129/1‑140 (MQ=255)                                                                                                                        cATTTAATGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGatat               <  1:284797/140‑1 (MQ=255)                                                                                                                             aaTGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCg          >  2:1362603/1‑140 (MQ=255)                                                                                                                               tGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGaaaaaa                          <  2:61166/122‑1 (MQ=255)                                                                                                                               tGACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGaaaaaa                          >  1:61166/1‑122 (MQ=255)                                                                                                                                gACGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTTCGTCATGTTGTATGGCAAAGGGGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTg       >  2:467202/1‑140 (MQ=255)                                                                                                                                 aCGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGc      <  2:1200055/140‑1 (MQ=255)                                                                                                                                  cGGGCGCTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGGCATGTTGTATTGCAAAGGGGCAGTGCCGGATGACGGAGCCGATTCAAATATGAAAAAACTGTTTGATATCCGCGGTGCg     >  2:1133561/1‑140 (MQ=255)                                                                                                                                     gcgcTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGtt        <  2:1103763/134‑1 (MQ=255)                                                                                                                                     gcgcTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGc  <  2:1250129/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         TGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGC  >  NC_000913/4142450‑4142721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑