Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,238,397	T→C	V255A (GTT→GCT)	yjbH →	YjbH family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,238,397	0	T	C	100.0%	349.0 / NA	99	V255A (GTT→GCT)	yjbH	YjbH family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (46/53);  total (46/53)

AGACGCCCTGGCAACCGCTGCGCCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGTTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGCCCGCCCGCAATATCAACCGCAGCCGCAGGATGCCATTTTGCA  >  NC_000913/4238262‑4238532                                                                                                                                        |                                                                                                                                        aGACGCCCTGGCAACCGCTGCGCCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAAc                                                                                                                                     <  1:1117334/140‑1 (MQ=255)  gACGCCCTGGCAACCGCTGCGCCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAAcc                                                                                                                                    >  2:91211/1‑140 (MQ=255)  gACGCCCTGGCAACCGCTGCGCCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAAcc                                                                                                                                    >  2:592950/1‑140 (MQ=255)  gACGCCCTGGCAACCGCTGCGCCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAAcc                                                                                                                                    >  2:1121393/1‑140 (MQ=255)  gACGCCCTGGCAACCGCTGCGCCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAAcc                                                                                                                                    <  1:1245342/140‑1 (MQ=255)   aCGCCCTGGCAACCGCTGCGCCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCt                                                                                                                                   <  1:384700/140‑1 (MQ=255)   aCGCCCTGGCAACCGCTGCGCCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCt                                                                                                                                   <  2:1223397/140‑1 (MQ=255)    cGCCCTGGCAACCGCTGCGCCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCtt                                                                                                                                  <  1:635317/140‑1 (MQ=255)     gCCCTGGCAACCGCTGCGCCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCt                                                                                                                                   <  2:1399440/138‑1 (MQ=255)     gCCCTGGCAACCGCTGCGCCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCt                                                                                                                                   >  1:1399440/1‑138 (MQ=255)       ccTGGCAACCGCTGCGCCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGc                                                                                                                               >  2:469969/1‑140 (MQ=255)         tGGCAACCGCTGCGCCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTa                                                                                                                             >  2:1051050/1‑140 (MQ=255)              aCCGCTGCGCCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAAc                                                                                                                        <  1:91211/140‑1 (MQ=255)                cGCTGCGCCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGt                                                                                                                      >  2:130066/1‑140 (MQ=255)                  cTGCGCCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAACTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTgg                                                                                                                    <  2:1347054/140‑1 (MQ=255)                   tGCGCCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAAc                                                                                                                                     >  1:128552/1‑122 (MQ=255)                   tGCGCCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAAc                                                                                                                                     <  2:128552/122‑1 (MQ=255)                    gcgcCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCa                                                                                                                  <  1:655577/140‑1 (MQ=255)                    gcgcCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCa                                                                                                                  <  1:1051050/140‑1 (MQ=255)                    ccgctgcaaaCTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCa                                                                                                                  <  2:1033433/133‑1 (MQ=255)                     cgcCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCaa                                                                                                                 >  2:809052/1‑140 (MQ=255)                     cgcCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCaa                                                                                                                 >  2:1520748/1‑140 (MQ=255)                     cgcCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCaa                                                                                                                 <  2:1128782/140‑1 (MQ=255)                     cgcCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCaa                                                                                                                 <  1:592950/140‑1 (MQ=255)                       ccTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAaca                                                                                                               <  2:867803/140‑1 (MQ=255)                         tGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACAcc                                                                                                             <  1:1346046/140‑1 (MQ=255)                                  aGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGttt                                                                                                    >  1:427514/1‑140 (MQ=255)                                      tGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCg                                                                                                >  2:314205/1‑140 (MQ=255)                                      tGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCg                                                                                                >  1:726505/1‑140 (MQ=255)                                      tGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCg                                                                                                >  2:511907/1‑140 (MQ=255)                                        aaGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGtt                                                                                              <  1:236063/140‑1 (MQ=255)                                         aGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTa                                                                                             <  1:130066/140‑1 (MQ=255)                                         aGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTa                                                                                             <  2:887464/140‑1 (MQ=255)                                         aGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTa                                                                                             <  1:324152/140‑1 (MQ=255)                                           gCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACg                                                                                           >  1:1355215/1‑140 (MQ=255)                                           gCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACg                                                                                           >  1:949533/1‑140 (MQ=255)                                            cAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGt                                                                                          <  2:209110/140‑1 (MQ=255)                                            cAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGt                                                                                          >  1:78622/1‑140 (MQ=255)                                            cAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGt                                                                                          >  1:1265758/1‑140 (MQ=255)                                            cAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGt                                                                                          >  2:429046/1‑140 (MQ=255)                                             aataatTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGtt                                                                                         <  2:796915/140‑1 (MQ=255)                                              ataatTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTg                                                                                        <  1:718081/140‑1 (MQ=255)                                              ataatTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTg                                                                                        >  1:789689/1‑140 (MQ=255)                                              ataatTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTg                                                                                        >  2:299501/1‑140 (MQ=255)                                              ataatTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTAGCAACACCTTTATGTTTGGCGTTACGTTg                                                                                        <  2:949974/140‑1 (MQ=255)                                              ataatTATCAGCAGGATTTTGCCGGAAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGtt                                                                                                     <  1:227373/127‑1 (MQ=255)                                              ataatTATCAGCAGGATTTTGCCGGAAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGtt                                                                                                     >  2:227373/1‑127 (MQ=255)                                                    aTCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCAcc                                                                                  <  2:78622/140‑1 (MQ=255)                                                    aTCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCAcc                                                                                  <  2:1265758/140‑1 (MQ=255)                                                    aTCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCAcc                                                                                  <  1:314205/140‑1 (MQ=255)                                                       agcagGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAAc                                                                               >  1:590194/1‑140 (MQ=255)                                                       agcagGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAAc                                                                               >  2:1160201/1‑140 (MQ=255)                                                         cagGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCAcc                                                                                  <  1:64788/135‑1 (MQ=255)                                                         cagGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCAcc                                                                                  >  2:64788/1‑135 (MQ=255)                                                         cagGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACtt                                                                             >  2:1041757/1‑140 (MQ=255)                                                         cagGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACtt                                                                             >  2:1451842/1‑140 (MQ=255)                                                            gATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGtt                                                                                         <  2:1448877/125‑1 (MQ=255)                                                            gATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGtt                                                                                         >  1:1448877/1‑125 (MQ=255)                                                            gATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTaa                                                                          >  2:635532/1‑140 (MQ=255)                                                            gATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTaa                                                                          >  2:960280/1‑140 (MQ=255)                                                            gATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTaa                                                                          >  1:374978/1‑140 (MQ=255)                                                                 tGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATc                                                                     <  2:794148/140‑1 (MQ=255)                                                                 tGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATc                                                                     >  1:754246/1‑140 (MQ=255)                                                                     gggAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTgcg                                                                 <  2:789689/140‑1 (MQ=255)                                                                      ggAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTgcgc                                                                <  1:744734/140‑1 (MQ=255)                                                                       gAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGcc                                                               <  2:754246/140‑1 (MQ=255)                                                                       gAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGcc                                                               <  2:590194/140‑1 (MQ=255)                                                                          gCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGt                                                            <  1:1520748/140‑1 (MQ=255)                                                                                  aaaaaaGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAAc                                                    <  1:469969/140‑1 (MQ=255)                                                                                    aaaaGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCTCGTCTTACAACGa                                                  >  2:1518650/1‑140 (MQ=255)                                                                                    aaaaGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGa                                                  >  2:825588/1‑140 (MQ=255)                                                                                    aaaaGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGa                                                  >  2:748035/1‑140 (MQ=255)                                                                                             tttAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAAcgcccg                                         <  2:1287903/140‑1 (MQ=255)                                                                                             tttAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAAcgcccg                                         <  2:556580/140‑1 (MQ=255)                                                                                                aaCGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACgcccgccc                                      <  1:257064/140‑1 (MQ=255)                                                                                                aaCGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACgcccgccc                                      <  1:809052/140‑1 (MQ=255)                                                                                                 aCGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGcccgcccg                                     >  2:449655/1‑140 (MQ=255)                                                                                                 aCGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGcccgcccg                                     <  1:1121393/140‑1 (MQ=255)                                                                                                 aCGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCCTACAACGATAACGcccgcccg                                     <  2:1088212/140‑1 (MQ=255)                                                                                                  cGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGCccgcccgc                                    <  1:299501/140‑1 (MQ=255)                                                                                                  cGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGCccgcccgc                                    >  2:789464/1‑140 (MQ=255)                                                                                                   gTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGCCCGCCCGCa                                   <  1:449655/140‑1 (MQ=255)                                                                                                             tttATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCTGTCTTACAACGATAACGCCCGCCCGCAATATCAAccg                         <  2:1355215/140‑1 (MQ=255)                                                                                                             tttATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGCCCGCCCGCAATATCAAccg                         <  1:429046/140‑1 (MQ=255)                                                                                                             tttATCGCGTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACAAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGCCCGCCCGCAATATCAAccg                         <  1:523622/140‑1 (MQ=255)                                                                                                                  cgcgTTACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGCCCGCCCGCAATATCAAccgcagcc                    >  1:638519/1‑140 (MQ=255)                                                                                                                      ttACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGCCCGCCCGCAATATCAAccgcagccgcag                >  2:56249/1‑140 (MQ=255)                                                                                                                       tACCGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGCCCGCCCGCAATATCAACCGCAGCCGCAgg               >  2:430171/1‑140 (MQ=255)                                                                                                                         ccGATTGGGCCGACGCTAACCTTAGCTATGAACGTTGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGCCCGCCCGCAATATCAACCGCAGCCGCAGGAt             >  2:64167/1‑140 (MQ=255)                                                                                                                          cGATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGCCCGCCCGCAATATCAACCGCAGCCGCAGGATg            <  1:635532/140‑1 (MQ=255)                                                                                                                           gATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGCCCGCCCGCAATATCAACCGCAGCCGCAGGATGc           >  1:1499470/1‑140 (MQ=255)                                                                                                                           gATTGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGCCCGCCCGCAATATCAACCGCAGCCGCAGGATGc           >  1:1299374/1‑140 (MQ=255)                                                                                                                             ttGGGCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGCCCGCCCGCAATATCAACCGCAGCCGCAGGATGCCa         <  1:1041757/140‑1 (MQ=255)                                                                                                                               gggCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGCCCGCCCGCAATATCAACCGCAGCCGCAGGATGCCAtt       >  2:176566/1‑140 (MQ=255)                                                                                                                                ggCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGCCCGCCCGCAATATCAACCGCAGCCGCAGGATGCCAttt      >  1:864400/1‑140 (MQ=255)                                                                                                                                 gCCGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGCCCGCCCGCAATATCAACCGCAGCCGCAGGATGCCAtttt     >  2:1115523/1‑140 (MQ=255)                                                                                                                                  ccGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGCCCGCCCGCAATATCAACCGCAGCCGCAGGATGCCATTTTg    <  1:1512203/140‑1 (MQ=255)                                                                                                                                  ccGACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGCCCGCCCGCAATATCAACCGCAGCCGCAGGATGCCATTTTg    <  2:638519/140‑1 (MQ=255)                                                                                                                                    gACGCTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGCCCGCCCGCAATATCAACCGCAGCCGCAGGATGCCATTTTgca  <  1:1160201/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        AGACGCCCTGGCAACCGCTGCGCCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGTTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGCCCGCCCGCAATATCAACCGCAGCCGCAGGATGCCATTTTGCA  >  NC_000913/4238262‑4238532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑