Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,320,364	A→G	R100R (CGT→CGC)	phnI ←	carbon‑phosphorus lyase core complex subunit PhnI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,320,364	0	A	G	100.0%	162.7 / NA	48	R100R (CGT→CGC)	phnI	carbon‑phosphorus lyase core complex subunit PhnI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/18);  total (30/18)

TCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCG  >  NC_000913/4320227‑4320499                                                                                                                                          |                                                                                                                                        tCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCg                                                                                                                                       >  1:855296/1‑140 (MQ=255)    gcggTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCAt                                                                                                                                    <  2:508791/140‑1 (MQ=255)    gcggTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCAt                                                                                                                                    <  2:209680/140‑1 (MQ=255)           tCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCggtg                                                                                                                             <  1:250481/140‑1 (MQ=255)                   ggCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAg                                                                                                                     <  1:666228/140‑1 (MQ=255)                    gcgcTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAACAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGc                                                                                                                    >  2:565759/1‑140 (MQ=255)                     cgcTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCTCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCg                                                                                                                   <  2:1427234/140‑1 (MQ=255)                        ttCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCt                                                                                                                <  2:855296/140‑1 (MQ=255)                           gCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAACAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGc                                                                                                             <  1:565759/140‑1 (MQ=255)                                          ggTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCg                                                                                              >  1:1431617/1‑140 (MQ=255)                                          ggTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCg                                                                                              >  1:293135/1‑140 (MQ=255)                                           gTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGc                                                                                             >  2:88922/1‑140 (MQ=255)                                             aaaaTCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCa                                                                                           <  2:1431617/140‑1 (MQ=255)                                                 tCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGTCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACgt                                                                                       <  2:828886/140‑1 (MQ=255)                                                    aGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACgtggt                                                                                    >  2:949402/1‑140 (MQ=255)                                                     gCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACgtggtg                                                                                   >  1:291743/1‑140 (MQ=255)                                                     gCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACgtggtg                                                                                   >  1:886981/1‑140 (MQ=255)                                                                   tAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGCGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCgcag                                                                     <  2:293135/140‑1 (MQ=255)                                                                   tAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGgcg                                                                          <  1:400449/135‑1 (MQ=255)                                                                   tAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGgcg                                                                          >  2:1121967/1‑135 (MQ=255)                                                                   tAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGgcg                                                                          <  1:1121967/135‑1 (MQ=255)                                                                   tAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGgcg                                                                          >  2:400449/1‑135 (MQ=255)                                                                   tAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCgcag                                                                     >  1:721784/1‑140 (MQ=255)                                                                   tAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCgcag                                                                     >  1:72907/1‑140 (MQ=255)                                                                         tAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGaa                                                               >  1:237640/1‑140 (MQ=255)                                                                         tAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGaa                                                               >  1:287569/1‑140 (MQ=255)                                                                          aGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGaaa                                                              >  1:666907/1‑140 (MQ=255)                                                                                ggCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCt                                                        >  2:1204412/1‑140 (MQ=255)                                                                                  ccAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTc                                                      <  1:949402/140‑1 (MQ=255)                                                                                      gcagcTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACg                                                  >  1:431428/1‑140 (MQ=255)                                                                                          cTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTAt                                              >  1:3628/1‑140 (MQ=255)                                                                                          cTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTAt                                              <  1:88922/140‑1 (MQ=255)                                                                                          cTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTAt                                              <  1:1204412/140‑1 (MQ=255)                                                                                               cgccgGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTc                                                      >  2:364645/1‑127 (MQ=255)                                                                                               cgccgGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTc                                                      <  1:364645/127‑1 (MQ=255)                                                                                                            tttATAAACGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTca                            >  2:1312057/1‑140 (MQ=255)                                                                                                                   aCGGCGGAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcagcgccag                     >  1:238848/1‑140 (MQ=255)                                                                                                                    cggcggAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcagcgccagc                    >  1:1437994/1‑140 (MQ=255)                                                                                                                    cggcggAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcagcgccagc                    >  1:1411043/1‑140 (MQ=255)                                                                                                                    cggcggAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcagcgcc                       <  1:1260648/137‑1 (MQ=255)                                                                                                                    cggcggAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcagcg                         <  1:109256/135‑1 (MQ=255)                                                                                                                    cggcggAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcagcg                         >  2:109256/1‑135 (MQ=255)                                                                                                                    cggcggAGATACGGCGTTCGAGGCGCATCCCGGGGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcagcgcc                       >  2:1260648/1‑137 (MQ=255)                                                                                                                      gcggAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcagcgccagcgc                  >  2:482510/1‑140 (MQ=255)                                                                                                                      gcggAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcagcgccagcgc                  >  1:1218288/1‑140 (MQ=255)                                                                                                                      gcggAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcagcgccagcgc                  >  2:935651/1‑140 (MQ=255)                                                                                                                      gcggAGATACGGCGTTCGAGGCGCATCCCGGTGGTGTAGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTcagcgctagcgc                  >  2:1356385/1‑140 (MQ=255)                                                                                                                             tACGGCGTTCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGt           <  1:1356385/140‑1 (MQ=255)                                                                                                                                    ttCGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGt    >  1:804293/1‑140 (MQ=255)                                                                                                                                      cGAGGCGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCg  <  2:1437994/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        TCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGACGCATCCCGGTGGTGTCGAGCGGCTCGCTTACCGCCAGCTTCGCCAACGTGGTGCGGTAGGCGCGCAGCAGGAAAATCGCTTCAACGTTATCGCCGCTGGCCTGTTTCAGCGCCAGCGCCGCCAGTTCGCGGTCG  >  NC_000913/4320227‑4320499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑