Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,329,001	C→T	P623S (CCC→TCC)	crfC →	clamp‑binding sister replication fork colocalization protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,329,001	0	C	T	100.0%	306.0 / NA	86	P623S (CCC→TCC)	crfC	clamp‑binding sister replication fork colocalization protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/47);  total (39/47)

TTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGCGCAGAAGTCGATCTCTATTGCCAACAAATGGCTAAAGTTCTGGCAGAGCAGGTCGATGAAT  >  NC_000913/4328867‑4329136                                                                                                                                       |                                                                                                                                        tttttCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGAc                                                                                                                                    <  1:328927/140‑1 (MQ=255)  ttttCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACt                                                                                                                                   <  2:1153946/140‑1 (MQ=255)      cACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTgggg                                                                                                                               <  1:1500006/140‑1 (MQ=255)         ggCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACg                                                                                                                            <  1:897573/140‑1 (MQ=255)           cGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACggg                                                                                                                          <  1:581418/140‑1 (MQ=255)           cGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACggg                                                                                                                          >  2:1154921/1‑140 (MQ=255)             tAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGa                                                                                                                        >  1:570690/1‑140 (MQ=255)             tAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGa                                                                                                                        >  1:1061849/1‑140 (MQ=255)              aGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGaa                                                                                                                       >  1:210830/1‑140 (MQ=255)                ttCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAAt                                                                                                                     >  2:1176083/1‑140 (MQ=255)                   cccACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATgag                                                                                                                  <  1:1104759/140‑1 (MQ=255)                   cccACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATgag                                                                                                                  >  2:231281/1‑140 (MQ=255)                     cACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGaa                                                                                                                >  1:444013/1‑140 (MQ=255)                       cTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAAt                                                                                                              >  2:645105/1‑140 (MQ=255)                       cTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAAt                                                                                                              >  2:1204349/1‑140 (MQ=255)                        ttCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATc                                                                                                             >  2:45467/1‑140 (MQ=255)                         tCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCa                                                                                                            <  2:444013/140‑1 (MQ=255)                          cAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCTCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCAc                                                                                                           >  1:700720/1‑140 (MQ=255)                          cAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCAc                                                                                                           >  2:1506637/1‑140 (MQ=255)                          cAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCAc                                                                                                           >  1:1309400/1‑140 (MQ=255)                          cAACACTCGCCAGCTGTTCAGGGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCAc                                                                                                           >  2:1087069/1‑140 (MQ=255)                               cTCGCCAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCTCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACa                                                                                                      <  2:700720/140‑1 (MQ=255)                                tCGCCAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAg                                                                                                     >  1:585121/1‑140 (MQ=255)                                 cGCCAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGa                                                                                                    <  2:1322229/140‑1 (MQ=255)                                  gCCAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGaa                                                                                                   >  2:23930/1‑140 (MQ=255)                                   ccAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGaaa                                                                                                  <  2:210830/140‑1 (MQ=255)                                   ccAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGaaa                                                                                                  <  1:1176083/140‑1 (MQ=255)                                   ccAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGaaa                                                                                                  <  1:1087069/140‑1 (MQ=255)                                     aGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACg                                                                                                <  1:1154921/140‑1 (MQ=255)                                     aGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACg                                                                                                <  2:803936/140‑1 (MQ=255)                                       cTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCATAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGt                                                                                              <  1:1380932/140‑1 (MQ=255)                                          ttCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCGCGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGGCTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGa                                                                                           >  2:1053934/1‑140 (MQ=255)                                          ttCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGaaa                                                                                                  >  2:1104777/1‑133 (MQ=255)                                          ttCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGaaa                                                                                                  <  1:1104777/133‑1 (MQ=255)                                          ttCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGa                                                                                           <  2:570690/140‑1 (MQ=255)                                          ttCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGa                                                                                           >  1:1028514/1‑140 (MQ=255)                                             aGTGAAGTAATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTa                                                                                        <  1:886871/140‑1 (MQ=255)                                              gTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTAc                                                                                       >  2:335277/1‑140 (MQ=255)                                               tGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACa                                                                                      <  1:618959/140‑1 (MQ=255)                                               tGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACa                                                                                      <  1:231281/140‑1 (MQ=255)                                                  aGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACa                                                                                      <  2:1323737/137‑1 (MQ=255)                                                  aGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACa                                                                                      >  1:1323737/1‑137 (MQ=255)                                                    tCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGaaa                                                                                                  <  1:1289430/123‑1 (MQ=255)                                                    tCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGaaa                                                                                                  >  2:1289430/1‑123 (MQ=255)                                                    tCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAgtggg                                                                                 >  1:609250/1‑138 (MQ=255)                                                    tCATTTCGCCCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGAGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGCCGGGGAAATGAGAAATCACCGACAGAAACGGGTGATTACActgtg                                                                                 >  2:578685/1‑140 (MQ=255)                                                          cgggcCAGGAAGCAACGGACGGGCAGAGCACGGGTTTAGGGGTTGTGCGTGAGACTTTTTCGCGCTGGGTGAAACAGGCCGGCTGGGGGCGGGGAAATGAGAAATTCCCGGCAGAAACGGTTGAATACAGTGTGTTGCaa                                                                           <  2:609250/137‑1 (MQ=255)                                                          cgcgCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCag                                                                           <  1:1023577/140‑2 (MQ=255)                                                           gcgcCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAAc                                                                          <  2:1049936/140‑1 (MQ=255)                                                              ccAGGAAGCAGCGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAg                                                                       >  1:162169/1‑140 (MQ=255)                                                              ccAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACa                                                                                      <  2:119351/125‑1 (MQ=255)                                                              ccAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACa                                                                                      >  1:119351/1‑125 (MQ=255)                                                                aGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCa                                                                     <  1:1204349/140‑1 (MQ=255)                                                                  gAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCAtt                                                                   <  2:1028514/140‑1 (MQ=255)                                                                         cGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGCGCa                                                            <  2:188497/140‑1 (MQ=255)                                                                         cGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGCGCa                                                            >  2:456749/1‑140 (MQ=255)                                                                           gACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCaa                                                                           <  2:548087/123‑1 (MQ=255)                                                                           gACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCaa                                                                           >  1:548087/1‑123 (MQ=255)                                                                             cGAGCAGAGCACGCGTTTATGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGCGCAGAAg                                                        <  1:1105536/140‑1 (MQ=255)                                                                             cGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACg                                                                         <  1:1343863/123‑1 (MQ=255)                                                                             cGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACg                                                                         >  2:1343863/1‑123 (MQ=255)                                                                              gagcagagcaCGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGTGCAGAAGt                                                       >  1:1122039/1‑140 (MQ=255)                                                                                gcagagcaCGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGCGCAGAAGTCg                                                     <  1:645105/140‑1 (MQ=255)                                                                                 cagagcaCGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGTGCAGAAGTCGa                                                    <  2:1122039/140‑1 (MQ=255)                                                                                    agcaCGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGCGCAGAAGTCGAtct                                                 <  2:1061849/140‑1 (MQ=255)                                                                                      caCGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGCGCAGAAGTCGAt                                                   <  1:123263/136‑1 (MQ=255)                                                                                      caCGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGCGCAGAAGTCGAt                                                   >  2:123263/1‑136 (MQ=255)                                                                                      caCGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGCGCAGAAGTCGATctct                                               >  2:648059/1‑140 (MQ=255)                                                                                      caCGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGCGCAGAAGTCGATctct                                               >  1:946249/1‑140 (MQ=255)                                                                                      caCGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGCGCAGAAGTCGATctct                                               >  1:1188102/1‑140 (MQ=255)                                                                                         gcgTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGCGCAGAAGTCGATCTCTAtt                                            <  2:1309400/140‑1 (MQ=255)                                                                                         gcgTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGCGCAGAAGTCGATCTCTAtt                                            >  1:451744/1‑140 (MQ=255)                                                                                         gcgTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGCGCAGAAGTCGATCTCTAtt                                            >  2:1151428/1‑140 (MQ=255)                                                                                            tttAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGCGCAGAAGTCGATCTCTATTGcc                                         <  2:162169/140‑1 (MQ=255)                                                                                            tttAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGCGCAGAAGTCGATCTCTATTGcc                                         <  2:451744/140‑1 (MQ=255)                                                                                                       tgCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGCGCAGAAGTCGATCTCTATTGCCAACAAATGGCt                              >  2:1303765/1‑140 (MQ=255)                                                                                                       tgCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGCGCAGAAGTCGATCTCTATTGCCAACAAATGGCt                              >  1:282568/1‑140 (MQ=255)                                                                                                        gCGTGAGACTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGCGCAGAAGTCGATCTCTATTGCCAACAAATGGCTa                             <  1:340592/140‑1 (MQ=255)                                                                                                            gagaCTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGCGCAGAAGTCGATCTCTATTGCCAACAAATGGCTAAAGt                         <  1:692381/140‑1 (MQ=255)                                                                                                               aCTTTTTCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGCGCAGAAGTCGATCTCTATTGCCAACAAATGGCTAAAGTTCt                      >  1:841487/1‑140 (MQ=255)                                                                                                                 tttttCGCGCTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGCGCAGAAGTCGATCTCTATTGCCAACAAATGGCTAAAGTTCTgg                    >  1:1141000/1‑140 (MQ=255)                                                                                                                       gcgcTGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGCGCAGAAGTCGATCTCTATTGCCAACAAATGGCTAAAGTTCTGGCAGAGc              <  1:1151428/140‑1 (MQ=255)                                                                                                                           tGGTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGCGCAGAAGTCGATCTCTATTGCCAACAAATGGCTAAAGTTCTGGCAGAGCAGGt          <  1:335277/140‑1 (MQ=255)                                                                                                                            ggTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGCGCAGAAGTCGATCTCTATTGCCAACAAATGGCTAAAGTTCTGGCAGAGCAGGTc         <  1:1303765/140‑1 (MQ=255)                                                                                                                             gTTGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTTTTGCAACGAGCATTAAGCGCAGAAGTCGATCTCTATTGCCAACAAATGGCTAAAGTTCTGGCAGAGCAGGTCg        <  2:946249/140‑1 (MQ=255)                                                                                                                               tGAATCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGCGCAGAAGTCGATCTCTATTGCCAACAAATGGCTAAAGTTCTGGCAGAGCAGGTAGAt      <  1:45467/140‑1 (MQ=255)                                                                                                                                   tCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGCGCAGAAGTCGATCTCTATTGCCAACAAATGGCTAAAGTTCTGGCAGAGCAGGTCGATGAAt  <  2:600547/140‑1 (MQ=255)                                                                                                                                   tCAGTCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGCGCAGAAGTCGATCTCTATTGCCAACAAATGGCTAAAGTTCTGGCAGAGCAGGTCGATGAAt  <  1:1053934/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        TTTTTCACGGCGTAGTTCCCCACTTCAACACTCGCCAGCTGTTCAGTGAAGTCATTTCGCGCCAGGAAGCAACGGACGAGCAGAGCACGCGTTTAGGCGTTGTGCGTGAGACTTTTTCGCGCTGGTTGAATCAGCCCGACTGGGGACGGGGAAATGAGAAATCACCGACAGAAACGGTTGATTACAGTGTGTTGCAACGAGCATTAAGCGCAGAAGTCGATCTCTATTGCCAACAAATGGCTAAAGTTCTGGCAGAGCAGGTCGATGAAT  >  NC_000913/4328867‑4329136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑