Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,341,014 A→G D205D (GAT→GAC)  melR ← DNA‑binding transcriptional dual regulator MelR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,341,0140AG100.0% 260.4 / NA 75D205D (GAT→GAC) melRDNA‑binding transcriptional dual regulator MelR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/35);  total (40/35)

ACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTG  >  NC_000913/4340878‑4341146
                                                                                                                                        |                                                                                                                                    
aCGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCa                                                                                                                                   <  1:1422302/140‑1 (MQ=255)
   cgcgAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAg                                                                                                                                >  2:71192/1‑140 (MQ=255)
   cgcgAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAg                                                                                                                                >  2:1025142/1‑140 (MQ=255)
      gAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGttt                                                                                                                             >  1:1099883/1‑140 (MQ=255)
      gAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGtt                                                                                                                              >  2:674626/1‑139 (MQ=255)
      gAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGtt                                                                                                                              <  1:674626/139‑1 (MQ=255)
       aaCGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGtttt                                                                                                                            >  1:323918/1‑140 (MQ=255)
          gTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCgg                                                                                                                         <  1:1534821/140‑1 (MQ=255)
             gTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCaa                                                                                                                      <  1:1099316/140‑1 (MQ=255)
                  tGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAAt                                                                                                                     >  2:162006/1‑136 (MQ=255)
                  tGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAAt                                                                                                                     <  1:162006/136‑1 (MQ=255)
                   gcgcATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGc                                                                                                                <  2:1099883/140‑1 (MQ=255)
                    cgcATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGcc                                                                                                               <  1:1080301/140‑1 (MQ=255)
                    cgcATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGcc                                                                                                               <  1:190560/140‑1 (MQ=255)
                     gcATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGccc                                                                                                              <  1:242187/140‑1 (MQ=255)
                            ggTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATa                                                                                                                    <  1:383497/127‑1 (MQ=255)
                            ggTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATa                                                                                                                    >  2:383497/1‑127 (MQ=255)
                               aaTGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGc                                                                                                    <  1:67819/140‑1 (MQ=255)
                               aaTGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGc                                                                                                    <  2:1304625/140‑1 (MQ=255)
                                   tACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAAc                                                                                                >  2:1479375/1‑140 (MQ=255)
                                   tACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAAc                                                                                                >  1:1003630/1‑140 (MQ=255)
                                     cTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACAt                                                                                              >  1:948031/1‑140 (MQ=255)
                                        tttCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTa                                                                                                  >  2:542309/1‑133 (MQ=255)
                                        tttCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTa                                                                                                  <  1:542309/133‑1 (MQ=255)
                                        tttCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATaaa                                                                                           >  1:939567/1‑140 (MQ=255)
                                              cGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTgcg                                                                                     >  1:100514/1‑140 (MQ=255)
                                               gTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTgcgc                                                                                    <  1:1025142/140‑1 (MQ=255)
                                                tCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCa                                                                                   >  2:1141689/1‑140 (MQ=255)
                                                        aTGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCga                                                                           <  1:974004/140‑1 (MQ=255)
                                                            cccGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACg                                                                       >  2:649933/1‑140 (MQ=255)
                                                            cccGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACg                                                                       <  2:1003630/140‑1 (MQ=255)
                                                            cccGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACg                                                                       >  2:217610/1‑140 (MQ=255)
                                                            cccGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACg                                                                       <  2:323918/140‑1 (MQ=255)
                                                               gCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTg                                                                    >  1:1260389/1‑140 (MQ=255)
                                                                 tGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGtt                                                                  >  1:402510/1‑140 (MQ=255)
                                                                             aTTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTgcg                                                      <  1:179039/140‑1 (MQ=255)
                                                                             aTTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTgcg                                                      <  2:402510/140‑1 (MQ=255)
                                                                              ttGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTgcgc                                                     >  2:116976/1‑140 (MQ=255)
                                                                              ttGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTgcgc                                                     >  2:516067/1‑140 (MQ=255)
                                                                              ttGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTgcgc                                                     >  1:194453/1‑140 (MQ=255)
                                                                               tGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGcgcg                                                    <  2:948031/140‑1 (MQ=255)
                                                                               tGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGcgcg                                                    <  1:649933/140‑1 (MQ=255)
                                                                                  aTAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAgg                                                 <  1:71192/140‑1 (MQ=255)
                                                                                   tAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGt                                                >  1:1490532/1‑140 (MQ=255)
                                                                                   tAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGt                                                >  2:1125190/1‑140 (MQ=255)
                                                                                      ttGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGtttt                                             >  2:48518/1‑140 (MQ=255)
                                                                                      ttGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGtttt                                             >  1:83183/1‑140 (MQ=255)
                                                                                      ttGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGtttt                                             >  1:1293354/1‑140 (MQ=255)
                                                                                         gCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTAtt                                          >  2:424809/1‑140 (MQ=255)
                                                                                           gTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTgcg                                                      >  1:184045/1‑126 (MQ=255)
                                                                                           gTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTgcg                                                      <  2:184045/126‑1 (MQ=255)
                                                                                           gTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGa                                        >  2:202023/1‑140 (MQ=255)
                                                                                            ttAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGAc                                       >  1:1387453/1‑140 (MQ=255)
                                                                                            ttAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGAc                                       >  1:593370/1‑140 (MQ=255)
                                                                                               aGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAg                                    <  2:100514/140‑1 (MQ=255)
                                                                                               aGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAg                                    >  2:432847/1‑140 (MQ=255)
                                                                                                gTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGa                                   >  1:1003517/1‑140 (MQ=255)
                                                                                                  ttGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAAt                                 <  1:116976/140‑1 (MQ=255)
                                                                                                      cGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAAt                                 >  2:93511/1‑136 (MQ=255)
                                                                                                      cGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAAt                                 <  1:93511/136‑1 (MQ=255)
                                                                                                       gTGCTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGtt                            <  2:83459/140‑1 (MQ=255)
                                                                                                          cTCAGCCACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTccc                         >  2:458585/1‑140 (MQ=255)
                                                                                                               ccACATCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCccagcca                    >  2:507762/1‑140 (MQ=255)
                                                                                                                cacaTCGTTGATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCccagccag                   <  1:1479375/140‑1 (MQ=255)
                                                                                                                         gATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTaa          >  2:143998/1‑140 (MQ=255)
                                                                                                                         gATGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTaa          >  2:415229/1‑140 (MQ=255)
                                                                                                                          aTGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTaaa         >  1:442428/1‑140 (MQ=255)
                                                                                                                           tGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAAt        <  2:83183/140‑1 (MQ=255)
                                                                                                                           tGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAAt        <  2:1387453/140‑1 (MQ=255)
                                                                                                                           tGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAAt        <  1:217610/140‑1 (MQ=255)
                                                                                                                           tGGTCAGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAAt        <  1:202023/140‑1 (MQ=255)
                                                                                                                                aGCGCCTGGTCATAGTTTTTGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGGGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTTTTGACCAGAATCGGTTCCCAGCCAGAGAGGCTTAATCGCtt   >  2:679928/1‑140 (MQ=255)
                                                                                                                                aGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCtt   <  2:1003517/140‑1 (MQ=255)
                                                                                                                                aGCGCCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCtt   <  1:744803/140‑1 (MQ=255)
                                                                                                                                 gcgcCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTTGGGGGCCGGGAGGTTTTTTTACCAAAAACCGGTCCCCACCCAAAGAGCCAAAATCGttta  >  2:364888/1‑139 (MQ=255)
                                                                                                                                 gcgcCTGGTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTg  <  1:1494638/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                    
ACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTG  >  NC_000913/4340878‑4341146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑