Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,386,805	C→T	E856K (GAA→AAA)	mscM ←	miniconductance mechanosensitive channel MscM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,386,805	0	C	T	100.0%	319.0 / NA	90	E856K (GAA→AAA)	mscM	miniconductance mechanosensitive channel MscM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (45/45);  total (45/45)

CTGCAATTTCGACCACTCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAA  >  NC_000913/4386668‑4386939                                                                                                                                          |                                                                                                                                       cTGCAATTTCGACCACTCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTca                                                                                                                                      <  2:287694/140‑1 (MQ=255)            aCCACTCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGc                                                                                                                           <  2:974806/140‑1 (MQ=255)            aCCACTCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGc                                                                                                                           >  1:527313/1‑140 (MQ=255)            aCCACTCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGc                                                                                                                           <  1:1496001/140‑1 (MQ=255)                cTCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGt                                                                                                                       >  2:186491/1‑140 (MQ=255)                          aTCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAg                                                                                                             >  2:800125/1‑140 (MQ=255)                          aTCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAg                                                                                                             >  1:68821/1‑140 (MQ=255)                          aTCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAg                                                                                                             >  1:265268/1‑140 (MQ=255)                           tCATTGAGAAGCCGACCAGCCCGCCAATCAGTATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGc                                                                                                            >  1:1034287/1‑140 (MQ=255)                           tCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTg                                                                                                                     <  2:470128/131‑1 (MQ=255)                           tCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTg                                                                                                                     >  1:470128/1‑131 (MQ=255)                           tCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGc                                                                                                            >  2:1470482/1‑140 (MQ=255)                            ccttGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCt                                                                                                           <  2:448724/138‑1 (MQ=255)                             aTTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTg                                                                                                          >  1:283342/1‑140 (MQ=255)                             aTTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTg                                                                                                          >  1:911491/1‑140 (MQ=255)                               tGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCg                                                                                                        <  2:68821/140‑1 (MQ=255)                               tGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCg                                                                                                        <  1:1205088/140‑1 (MQ=255)                               tGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCg                                                                                                        >  2:1327459/1‑140 (MQ=255)                               tGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCg                                                                                                        >  2:189709/1‑140 (MQ=255)                                 agaAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAg                                                                                                             <  1:1406653/133‑1 (MQ=255)                                 agaAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAg                                                                                                             >  2:1406653/1‑133 (MQ=255)                                 agaAACCGACCAGCCCGCTAAGCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCgtg                                                                                                      >  2:1088362/1‑140 (MQ=255)                                  gaAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGTTGCgtgg                                                                                                     <  1:800125/140‑1 (MQ=255)                                  gaAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCgtgg                                                                                                     <  2:660940/140‑1 (MQ=255)                                    aGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCACCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCgtggtg                                                                                                   <  2:1236439/140‑1 (MQ=255)                                     gCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGtga                                                                                                  <  2:1381486/140‑1 (MQ=255)                                      ccGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAg                                                                                                             <  1:1291672/128‑1 (MQ=255)                                      ccGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAg                                                                                                             >  2:1291672/1‑128 (MQ=255)                                       cGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGtgatg                                                                                                <  1:189709/140‑1 (MQ=255)                                         aCCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGc                                                                                                         >  2:1378951/1‑129 (MQ=255)                                         aCCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGc                                                                                                         <  1:1378951/129‑1 (MQ=255)                                         aCCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTgatgat                                                                                              >  2:105845/1‑140 (MQ=255)                                         aCCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTgatgat                                                                                              >  2:302264/1‑140 (MQ=255)                                          ccAGCCCGCCAATCAGTATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATa                                                                                             <  2:1034287/140‑1 (MQ=255)                                            aGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATaaa                                                                                           <  2:911491/140‑1 (MQ=255)                                             gCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCg                                                                                                        <  1:224555/126‑1 (MQ=255)                                             gCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCg                                                                                                        >  2:224555/1‑126 (MQ=255)                                               ccGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAacac                                                                                        <  1:1405309/140‑1 (MQ=255)                                                    aaTCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGaa                                                                                   <  2:265268/140‑1 (MQ=255)                                                            tcagcaGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATc                                                                           >  2:329705/1‑140 (MQ=255)                                                                cagATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAgca                                                                       >  1:1428519/1‑140 (MQ=255)                                                                cagATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAgca                                                                       >  1:277701/1‑140 (MQ=255)                                                                cagATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAgca                                                                       <  2:399349/140‑1 (MQ=255)                                                                 agATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAgcac                                                                      <  1:329705/140‑1 (MQ=255)                                                                  gATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAgcacc                                                                     >  2:1452736/1‑140 (MQ=255)                                                                    tatTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAgcaccgc                                                                   >  1:948194/1‑140 (MQ=255)                                                                    tatTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAgcaccgc                                                                   >  1:162713/1‑140 (MQ=255)                                                                     atTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAgcaccgca                                                                  <  1:1452736/140‑1 (MQ=255)                                                                        tggtgatggtggtgatggCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGcaccgcaccg                                                               >  2:1019588/1‑140 (MQ=255)                                                                         ggtgatggtggtgatggCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAgcac                                                                      <  2:1462903/132‑1 (MQ=255)                                                                         ggtgatggtggtgatggCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAgcac                                                                      >  1:1462903/1‑132 (MQ=255)                                                                          gtgatggtggtgatggCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAg                                                             >  2:1120181/1‑140 (MQ=255)                                                                             atggtggtgatggCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGt                                                          >  1:679569/1‑140 (MQ=255)                                                                                gtggtgatggCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAAt                                                       <  2:527313/140‑1 (MQ=255)                                                                                 tggtgatggCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATAAGCACCGCACCGAGGGTAAtt                                                      >  2:1395022/1‑140 (MQ=255)                                                                                          cGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGa                                             >  1:554018/1‑140 (MQ=255)                                                                                           gTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGTTGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGAc                                            >  1:236170/1‑140 (MQ=255)                                                                                           gTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGAc                                            <  1:1120181/140‑1 (MQ=255)                                                                                           gTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGAc                                            <  2:283342/140‑1 (MQ=255)                                                                                              aCCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACttt                                         >  2:444235/1‑140 (MQ=255)                                                                                                ccGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCt                                       <  1:1019588/140‑1 (MQ=255)                                                                                                ccGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCt                                       <  1:698407/140‑1 (MQ=255)                                                                                                 cGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTa                                      <  1:186491/140‑1 (MQ=255)                                                                                                   tACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACg                                    >  2:468277/1‑140 (MQ=255)                                                                                                   tACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACg                                    >  1:437796/1‑140 (MQ=255)                                                                                                   tACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGAACCGCACCGAGGGTAATTGGCTCCAGACTTTCt                                       >  1:29222/1‑137 (MQ=255)                                                                                                   tACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGAACCGCACCGAGGGTAATTGGCTCCAGACTTTCt                                       <  2:29222/137‑1 (MQ=255)                                                                                                     cccGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGcc                                  <  2:679569/140‑1 (MQ=255)                                                                                                       cGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCt                                <  1:444235/140‑1 (MQ=255)                                                                                                        ggCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCt                                       <  1:862055/132‑1 (MQ=255)                                                                                                        ggCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCt                                       >  2:862055/1‑132 (MQ=255)                                                                                                        ggCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTg                               <  1:104411/140‑1 (MQ=255)                                                                                                         gCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCATCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGt                              >  2:1309887/1‑140 (MQ=255)                                                                                                         gCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCt                                       <  2:1377285/131‑1 (MQ=255)                                                                                                         gCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCt                                       >  1:1377285/1‑131 (MQ=255)                                                                                                          cGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTa                             <  1:566447/140‑1 (MQ=255)                                                                                                          cGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTa                             >  1:1381195/1‑140 (MQ=255)                                                                                                          cGTTAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTa                             >  1:1533955/1‑140 (MQ=255)                                                                                                            ttAAATCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTAcc                           <  2:554018/140‑1 (MQ=255)                                                                                                                aTCCAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTgg                       <  2:437796/140‑1 (MQ=255)                                                                                                                   cAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAgg                    <  2:948194/140‑1 (MQ=255)                                                                                                                   cAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAgg                    <  1:1327459/140‑1 (MQ=255)                                                                                                                   cAGGTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAgg                    <  1:105845/140‑1 (MQ=255)                                                                                                                      gTGCTGCAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGa                 >  1:1445571/1‑140 (MQ=255)                                                                                                                         ctgcAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACAt              >  1:1289259/1‑140 (MQ=255)                                                                                                                          tgcAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGCAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATc             >  1:674906/1‑140 (MQ=255)                                                                                                                           gcAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATcc            >  2:37351/1‑140 (MQ=255)                                                                                                                           gcAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATcc            >  1:778417/1‑140 (MQ=255)                                                                                                                            cAAAATCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATccc           >  2:1124961/1‑140 (MQ=255)                                                                                                                             aaaaTCGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCca          <  2:1533955/140‑1 (MQ=255)                                                                                                                                  cGCCAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCg     <  2:1428519/140‑1 (MQ=255)                                                                                                                                     cAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGaaa  <  1:208728/140‑1 (MQ=255)                                                                                                                                     cAGTTTCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGaaa  <  1:1124961/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       CTGCAATTTCGACCACTCAATACCAATCATTGAGAAGCCGACCAGCCCGCCAATCAGCATCAGCAGATATTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAA  >  NC_000913/4386668‑4386939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑